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한우의 반복 과배란 및 산차가 수정란 생산에 미치는 영향 KCI 등재

Effect of Multiple Superovulation and Parity on Embryo Production in Hanwoo

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한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

본 연구는 소에 있어서 다량의 수정란을 생산하여, 고능력의 유전인자를 조기에 확산할 수 있도록, 한우 공란우의 반복 과배란을 유도하여 안정적이고 효율적인 수정란 생산을 목적으로 실시하였으며, 그 결과는 다음과 같다. 1. 한우 경산우 및 미경산우를 4회 반복적으로 과배란 처리하였을 때, 수정란 채란이 가능한 공란우는 였으며, 3회 처리시 다소 낮아, 약간의 유의차가 인정되었다(P<0.05). 2. 한우 경산우 및 미경산우를 4회 반복적으로 과배란 처리하

This study was performed to investigate the effects of multiple superovulation and parity on embryo production in Hanwoo cows. Donors were superovulate 4 times months interval and inserted CIDR plus (with the capsule of estradiol benzoate 10mg) on Day 10 from standing heat for 9 days and injected 2.5ml FSH (Antorin R-10) 2 times in a day on 6th day to 10th day from insertion of CIDR and the doses of FSH were decreasing 0.5ml on every 2 times. On 3th day of FSH injection, 25ml were injected i.m. and on 4th day, CIDR was removed. After 2 days from removing CIDR, AI was performed 2 times 12 hour apart with 2 straws of Korean Proved frozen Semen and simultaneously 200ug/ml GnRH was injected and embryos were recovered on 7th day from Al. The response rates of superovulated donors were from 1 to 4 times of superovulation, respectively. There were significant differences among No. of superovulation times (P<0.05). The results of transferable embryos were 3.7, 3.4, 3.4, 5.7 from 1 to 4 times of superovualtion, respectively. There were no differences among No. of superovulation times. The results of transferable embryos were 2.5, 3.0, 5.3, 3.0, 3.4 form heifer, first born to 4 the born, respectively. There were significant differences among the parities of donors (P<0.05). These results suggested that even 4 times of superovulations of Hanwoo donors could be able to recover transferable embryos, it might be used the donors maximally and improved the adaptation of embryo transfer to farms safely.

저자
  • 최선호(농촌진흥청 축산연구소 가축유전자원시험장)
  • 류일선(농촌진흥청 축산연구소 가축유전자원시험장)
  • 손동수(농촌진흥청 축산연구소 가축유전자원시험장)
  • 조상래(농촌진흥청 축산연구소 가축유전자원시험장)
  • 한만희(농촌진흥청 축산연구소 가축유전자원시험장)
  • 김현종(농촌진흥청 축산연구소 가축유전자원시험장)
  • 최창용(농촌진흥청 축산연구소 가축유전자원시험장)
  • 김영근(농촌진흥청 축산연구소 가축유전자원시험장)