논문 상세보기
pp.127-131
발정주기와 상관없이 CIDR을 삽입하는 날에 50 mg progesterone, 2.5 mg estradiol benzoate를 근육주사하였다. CIDR 삽입 후 4, 5일에 28 AU FSH (Antorin R10)을 4일 동안 감량법으로 주사하였다. 6, 7회 FSH 주사 후 25 mg, 15 mg 를 각각 주사한 다음, CIDR는 7회 FSH 주사 후 제거하였다. 1회째 주사 후 48시간에 GnRH를 주사하였다. 공란우는 발정확인 후 12시간 간격
This study was performed to investigate the effects of parity and season on the embryo production in superovulated Hanwoo cows. Superovulation was performed from 1 to 8 times by repeated superovulation treatment of Hanwoo cows (n = 22). Irrespective of estrous cycle, donor cows were received a CIDR, progesterone (50 mg) and estradiol benzoate (2.5 mg). After 4.5 days, the donor cows were superovulated with total 28AU FSH (Antorin R-10) administrated twice daily in a decreasing dose for 4 days. On and of FSH injection, 2.5 mg and 15 mg were injected i.m, respectively. CIDR was removed at the FSH injection. The donor cows received GnRH at 48 hrs after injection. The donor cows were artificially inseminated three times after estrous detection at 12 hr intervals and embryos were recovered 7 days after estrous detection. The mean number of total ova, transferrable embryos, degenerated embryos and unfertilized oocytes were , , and per donor cows, respectively. A higher number of total ova were recovered in parity 3~5 () than 1~2 (, P<0.05). The number of recovered normal embryos is significantly higher in parity 3~5 () than that of over 6 (). Significantly higher number of total ova and normal embryos were recovered in summer (, ) than in autumn (, ) and winter (, ), respectively (P<0.05). Transferable embryos were significantly higher in summer () than in winter (, P< 0.05). The results were showed that parity and season affecting on the production of embryos in superovulated Hanwoo.
