산업식품공학 제25권 제4호 (p.305-312)

Gluco-Oligosaccharides from Weissella cibaria as Potential Prebiotics

키워드 :
lactic acid bacteria,Weissella cibaria,gluco-oligosaccharides,probiotics,prebiotics

목차

Abstract
Introduction
Materials and Methods
   Bacterial isolation and cultivation
   Identification of oligosaccharide-producing LAB
   Production and purification of oligosaccharidesfrom W. cibaria YRK005
   Determination of the oligosaccharide structure
   Probiotic strains and culture media
   Media for probiotic strains for the determination ofthe prebiotic effect
   Growth of probiotic strains for the determination ofthe prebiotic effect
   Statistical analysis
Results and Discussion
   Screening of oligosaccharide-producing LAB
   Identification of oligosaccharide-producing strain
   Determination of the structure of oligosaccharidesfrom W. cibaria YRK005
   Analysis of molecular mass and monosaccharidecomposition of oligosaccharides
   Determination of glycosidic linkages in oligosaccharidesby enzymatic digestion
   Determination of glycosidic linkages in oligosaccharidesby 1H-NMR spectroscopy
   Prebiotic effects of oligosaccharides
References

초록

Oligosaccharide-producing lactic acid bacteria were isolated from young radish and identified as Weissella cibaria YRK005. Oligosaccharides from W. cibaria YRK005 were purified using size-exclusion high-performance liquid chromatography, and their structure was determined. The oligosaccharides consisted of glucose only, 1 H-nuclear magnetic resonance analysis revealed that the oligosaccharides consisted of 93.8% α-1,6 glycosidic linkages and 6.2% α-1,4 glycosidic linkages, and molecular weight was determined to be 1.1210 2 Da. When the oligosaccharides' prebiotic effect on probiotic strains was examined, the oligosaccharides promoted the growth of four probiotic strains, Bifidobacterium adolescentis, Lactobacillus acidophilus, Lactococcus lactis, and Lactobacillus pentosus, compared to fructo-oligosaccharides as a reference. These results indicated that oligosaccharides from W. cibaria YRK005 could be used as potential prebiotics.