한국식품영양학회지 Vol. 35 No. 2 (p.116-126)

LPS로 유도된 RAW 264.7 대식세포에서 청대의 항염증효과

Anti-Inflammatory Effect of Chung-Dae in LPS-Treated RAW 264.7 Cells
키워드 :
anti-inflammatory effect,Chung-Dae,indigo pulverata levis,Polygonum tinctorium Lour.,LPS-treated RAW 264.7 cells

목차

Abstract
서 론
재료 및 방법
   1. 시료 추출물 제조
   2. Raw 264.7 대식세포 배양
   3. MTT assay를 이용한 세포 성장 억제효과
   4. NO 생성능 측정
   5. Enzyme-linked immunosorbent(ELISA) assay 이용한염증관련 사이토카인 측정
   6. RT-qPCR을 이용한 염증관련 유전자의 mRNA level측정
   7. Western blot을 이용한 AGS 세포 내 유전자의 단백질발현 측정
   8. 통계 처리
결과 및 고찰
   1. 청대의 cell viability 측정
   2. 청대의 LPS로 유도된 NO 생성능 억제
   3. 청대추출물이 전염증성 사이토카인 함량에 미치는 영향
   4. 청대추출물이 전염증성 사이토카인 및 염증 관련효소mRNA 발현에 미치는 영향
   5. 청대추출물이 전염증성 사이토카인 및 염증 관련protein 발현에 미치는 영향
요약 및 결론
References

초록

The purpose of this study was to analyze the anti-inflammatory effect of Chung-Dae Indigo Pulverata Levis, indigo naturalis) produced during indigo dyeing. As a result of in vitro cytotoxicity experiments using RAW 264.7 cell, Chung-Dae extract did not inhibit cell proliferation in Raw 264.7 cells in the range of 1~32 μg/mL. NO production was significantly reduced when Chung-Dae extracts were treated at concentrations of 2, 8, and 32 μg/mL (p<0.05). The pro-inflammatory cytokines TNF-α, IL-6, IL-1β and IFN-γ significantly decreased when the Chung-Dae extract was treated at concentrations of 2, 8, and 32 μg/mL compared to the LPS group, and similarly, the TNFα and IL-6 mRNA levels also decreased. Additionally, the mRNA level of COX-2 was also suppressed. At the protein expression level, the expression of TNF-α, IL-6, iNOS and COX-2 were observed with LPS and Chung-Dae extract significantly decreased compared to the group treated with only LPS (p<0.05). From the above results, it shows that Chung-Dae extract, a plant-derived compound, inhibits the inflammatory response induced by LPS in RAW 264.7 cells. and in particular, regulates the inflammatory response by inhibiting the expression of pro-inflammatory cytokines and inflammation-related enzymes.