Foxglove aphid, Aulacorthum solani (Kaltenbach), is a Hemipteran insect that infected a wide variety of plants worldwide and caused serious yield losses in crops. The objective of this study was to identify the putative QTL for foxglove aphid resistance in wild soybean, PI 366121, (Glycine soja Sieb. and Zucc.). One hundred and forty one F2-derived F8 recombinant inbred lines developed from a cross of susceptible Williams 82 and resistant PI 366121, were used. The phenotyping of antibiosis and antixenosis was done through choice and no-choice assays with total plant damage (TPD) and primary infestation leaf damage (PLD); a genome-wide molecular linkage map was constructed with 504 single nucleotide polymorphism markers utilizing a GoldenGate assay. Using inclusive composite interval mapping analysis for foxglove aphid resistance, one major candidate QTL on chromosome 7 and 3 minor QTL regions on chromosome 3, 6 and 18 were identified. The major QTL on chromosome 7 showed both antixenosis and antibiosis resistance responses. However, the minor QTLs showed only antixenosis resistance response. The major QTL mapped to a different chromosome than the previously identified foxglove aphid resistance QTL, Raso1, from the cultivar Adams. Also, the responses to the Korea biotype foxglove aphid were different for Raso1, and the gene from PI 366121 against the Korea biotype foxglove aphid were different. Thus the foxglove aphid resistance gene from PI 366121 was determined to be an independent gene to Raso1 and designated to Raso2. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars.

Foxglove aphid, Aulacorthum solani (Kaltenbach), is a Hemipteran insect that infected a wide variety of plants worldwide and caused serious yield losses in crops. The foxglove aphid resistance gene, Raso2 was previously mapped from PI 366121 (Glycine soja Sieb. and Zucc.) to a 26cM marker interval on soybean chromosome 7. The development of additional genetic markers, which are mapped closer to Raso2 were required to accurately position the gene to improve the effectiveness of marker assisted selection. The objective of this study was to narrow down the putative QTL region, which is responsible to foxglove aphid resistance in PI366121 using recently developed high-density 180K Axiom SoyaSNP genotyping array. One hundred and forty one F8-derived F12 recombinant inbred lines developed from a cross of susceptible Williams 82 and resistant PI 366121, were used to generate a fine map of Raso2 interval. The phenotyping of antibiosis and antixenosis was done through choice and no-choice assays with total plant damage (TPD) and primary infestation leaf damage (PLD). The composite interval mapping analysis showed that the physical interval between two flanking makers, which was corresponding to Raso2, was narrowed down to 500kb on the Williams 82 genome assembly (Glyma2.0), instead of 4Mb in the previous report using Goldengate assay. In the Raso2 interval, there are about 60 candidate genes, including 4 of NBS-containing putative R genes. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars.

The depletion of stratospheric ozone has resulted in increased amount of ultraviolet-B radiation (UV-B: 280-320 nm) reaching the Earth’s surface and could cause significant biological effect in plants. In this study, putative quantitative trait loci (QTL), which is responsible to UV-B resistance in soybean, was identified using recently developed high-density 180K Axiom SoyaSNP genotyping array. A population of 115 recombinant inbred lines (RILs) derived from a cross between susceptible Keunolkong and resistant Iksan 10 was analyzed. A total 8,970 polymorphic SNP markers were used to construct linkage map. The both parents and RILs were grown with supplemental UV-B radiation in a greenhouse condition. Three categories of UV-B induced morphological damage, degree of leaf chlorosis, leaf shape change, and total plant damage were evaluated. Using composite interval mapping analysis, one major QTL associated with all of the phenotypic traits was detected on 7.7cM of soybean chromosome 7 with 22 of LOD score accounting for about 60% of phenotypic variance. Also, the allele from Iksan 10 were responsible for the UV-B resistance. Thus, the UV-B resistance QTL on chromosome 7 from Iksan 10 was designated to qUVBR1, corresponding to 30kb on the Williams 82 genome assembly (Glyma2.0) including 7 candidate genes. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars. In addition, these results provided useful information not only for marker-assisted selection for UV-B resistance soybean, but also for the future identification of putative candidate genes, responsible for UV-B resistance in soybean.

Efforts were made to identify the optimum operational condition of Semi-continuously Fed and Mixed Reactor(SCFMR) to treat the dairy cow manure and saw dust mixture. Step-wise increase in organic loading rates (OLRs) and decrease in hydraulic retention times (HRTs) were utilized until the biogas volume became significantly decreased in SCFMR at mesophilic temperature (35℃). The optimum operating condition of the SCFMR fed with TS 15% dairy cow manure and saw dust mixture was found to be at HRTs of 30 days and its corresponding OLRs of 4.27 kgVS/m³-day. The optimum ranges of biogas and methane production rates were 1.47 volume of biogas per volume of reactor per day(v/v-d) and 1.14 v/v-d, respectively. This result was due to the high alkalinity concentration of SCFMR fed with the original substrate, dairy cow manure and saw dust mixture whose alkalinity was more than 10,000 mg/L as CaCO3. The parameters for the reactor stability, the ratios of volatile acids and alkalinity concentrations (V/A) and the ratio of propionic acid and acetic acid concentrations (P/A) appeared to be 0.07-0.09 and 0.38-0.43, respectively, that were greatly stable in operation. The Total Volatile Solids(TVS) removal efficiency based on the biogas production was 45.2% at the optimum HRTs. Free ammonia toxicity was not experienced at above 160 mg/L due to the acclimation of high concentration of ammonia by the high reactor TS content above 9.0%.

This study analyzes the buckling safety in the area of circumferentially edge-stiffened door openings without any additional longitudinal stiffeners of offshore tubular steel towers. The tubular steel tower is subjected to six (6) different load situations which are deemed to be normal and abnormal operating cases for the ultimate limit state. Analytical method using parametric equations based on Eurocode 3 - Design of Steel Structures and numerical method of finite element are used to analyze the critical meridional buckling stress. ABAQUS, a finite element program, is used for the numerical method analysis. Buckling safety analysis in the localized area near the opening is studied, and points of interest are defined for comparison between the two aforementioned analyses. Findings are tabulated and shown in illustrative charts, and conclusions are made.

This parametric study investigates the effect of significant parameters on the elastic global lateral torsional buckling capacity of horizontally curved twin I-girder systems. Included are the effect of curvature and the effect of girder spacing. Twin I-girder systems analyzed in this study are those which are interconnected by intermediate cross frames and are subjected to uniform moment. The finite element analysis software, ABAQUS, is used to model the horizontally curved twin I-girder system and conduct the buckling analysis. To see the significant effect of curvature and girder spacing, the results from the finite element analysis of horizontally curved twin I-girder systems are compared to the results of a closed form solution for straight twin I-girder systems. The findings of this parametric study will be shown using illustrative figures and tabulated data and conclusions are made.