In Korea, twelve species in genus Myrmica have been described. Some of them are morphologically similar and this makes their identification difficult. For this reason, we collected several Myrmica species in question and inferred their phylogenetic relationship using the 418bp partial COI (cytochrome C oxidase 1) region from a total of 33 individuals. We found that the CO1 haplotypes are effectively grouped into three clusters that match well to their external morphological characters. Although this three species could be distinguished by the only small part of the COI region, the two individual sample of the M. kotokuii and one sample of the M. carinata are included in the M. kurokii group. The results indicate that the morphological identification could be obscure in the three species and it requires a close examination for this phenomenon.

Wolbachia is one of the most common endosymbionts best known to induce several reproductive alterations in its insect hosts. In some cases, the insect hosts harbor more than two strains of the bacterium. The Vollenhovia emeryi ant lives in dead trees and is morphologically subdivided into the long-winged and the short-winged. Interestingly the short-winged morph is free of Wolbachia, but only the long-winged morph is multiple- infected with the Wolbachia bacterium. We sampled four populations of the long-winged morph in Korea and performed pyrosequencing in Multilocus Sequence Typing (MLST), to determine the bacterial strain diversity. Six different gene regions (coxA, fbpA, ftsZ, gatB, hcpA and wsp gene) were targeted and amplified. However, the result shows that diversity of haplotypes is very high. The pyrosequencing approach in MLST, a new method of discriminating Wolbachia strains, is promising to effectively detect multiple infections and rare haplotypes.

The hologenome theory of evolution proposes that an organism is simply not an individual but the totality of numerous microbial symbionts to the host. In fact, the evidence of biochemical and physiological associations between the symbionts and the host has been growing fast in all major taxa. The Vollenhovia emeryi ant is tiny and found nationwide in Korea. The ant can be further categorized by its wing morphology, eg. long-winged and short-winged. Our initial screening process showed that the microbial reproductive manipulator, the Wolbachia bacterium, only infected the long-winged morph. This gave us a good opportunity to investigate the effect of the Wolbachia infection on the bacterial community diversity using the next generation sequencing technique. We find that there are about 180 bacterial symbionts in the short-winged morph. On the other hand, the long-winged morph has only about 20 bacterial symbionts. This implies that the bacterial community diversity may be subject to the existence of Wolbachia. Furthermore, the Wolbachia strain diversity is unexpectedly high. In addition, the bacterial structure difference among castes indicates that there may be labour division even between queens. The results and future research direction will be discussed from the hologenome theory perspective.

Microtubule-associated protein 1B (MAP1B), a member of MAP1 family, plays a key role in neuronal development. MAP1B binds to many kinds of proteins directly or indirectly. This study was performed to investigate whether MAP1B interacts with GAPDH in bovine follicles using immunoprecipitation (IP) with Western blot analysis and immunohistochemisty. The mRNA expressions of MAP1B and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) were down-regulated in bovine follicular cystic follicles (FCF). In parallel with the mRNA levels, their protein levels were also down-regulated in FCFs. In addition, MAP1B and GAPDH were co-localized at the cytoplasm of follicles. IP with Western blot analysis showed that MAP1B bound to GAPDH in normal follicles, but their binding was absent in FCFs, suggesting a low level of MAP1B and/or GAPDH expressions in FCFs. Taken together, these results suggest that MAP1B interacted with GAPDH may play a role in bovine follicle development, and that GAPDH does not function always as a loading control in bovine follicles.

An increase in population initiating rapid industrialization was found to consequently in-crease the effluentsand domestic wastewater into the aquatic ecosystem. In this research the potentialities of Sambucus nigra L. (SNL) plant in the remediation of water, contaminated with methylene blue (MB), a basic dye were investigated. SNL was chemically impregnated with KHCO3. Operating variables studied were pH, amount of adsorbent and contact time. In general, pH did not have any significanteffect on colour removal and the highest adsorp-tion capacity was obtained in 0.035 g MB/g-activated carbon. The Langmuir, Freundlich, Temkin and Dubinin-Radushkevich adsorption models were applied to describe the equi-librium isotherms. The adsorption isotherm data were fittedto the Temkin isotherm. The mass transfer property of the sorption process was studied using Lagergren pseudo-firstorder and chemisorption pseudo-second-order kinetic models. The sorption process obeyed the pseudo-second-order kinetic model. The surface area, pores volume and diameter were assessed by the Brunauer-Emmett-Teller and Barrett-Joyner-Halenda methods. The results were compared to those from activated carbon (Merck) and an actual sample. The results indicate that SNL can be employed as a natural and eco-friendly adsorbent material for the removal of dye MB from aqueous solutions.

As a specialty oil, furan fatty acids have gained special attentions since they are known to play important roles in biological systems including human. Although several studies reported chemical synthesis of furan fatty acids, their synthesis consisted of complicated chemical multistep with chemical catalysts. Recently, a simple one-step heat treatment method was developed to produce a novel furan fatty acid, 7,10-epoxy-octadeca-7,9-dienoic acid (7,10-EODA) from a dihydroxyl fatty acid 7,10-dihydroxy-8(E)-octadecenoic acid (DOD). In this report we studied about optimization of environmental conditions for the maximum production of 7,10-EODA from DOD by heat treatment. Production of 7,10-EODA was maximized at over 85℃ for at least over 48 hour in hexane. Solvent volume for maximum production should be over 300 mL per 10 mg DOD.

The current study aimed to describe overall trends in recent English second language (L2) academic writing research and to identify effective interventions. To support drawing defensible conclusions based on the literature dealing with L2 writing, only recent empirical articles dealing with academic writing for English-L2 college students published in peer reviewed journals were included. Fifty five English L2 writing articles met the criteria for inclusion. For the identification of effective L2 writing interventions, I discriminated if the studies provided L2 writing interventions and the relevance to L2 writing development. As a result of analyses based on themes emerging in the English L2 academic writing literature, I noted effective English L2 academic writing interventions. Those interventions were teacher feedback, self-regulatory learning, peer feedback, and technology use. The use of a variety of measures and incorporation of specificity about prompts into the studies was recommended and the developmental trajectory of L2 writers was suggested to be further studied.

The present study was to assess the in vitro viability and sexing rate of bovine embryos. Blastocysts were harvested on day 7～9 day after insemination(in vitro and in vivo), and the sex of the embryos was examined using the LAMP method. Embryo cell biopsy was carried out in a 80 μl drop Ca2+, Mg2+ free D-PBS and, biopsied embryos viability were evaluated after more 12 h culture in IVMD culture medium. The formation of recovered embryo to expanded and hatching stages had ensued in higher of sexed embryo in vivo than in vitro (100% vs. 89%, p<0.05), and in vitro, the rates of degeneration after sexing were significantly (p<0.05) higher in vitro than in vivo(11% vs. 0.0%). The rates of the predicted sex were female 61% vs. 56%, and male 39% vs. 44% in vivo and in vitro, respectively. The rates of survival following different biopsy methods were seen between punching and bisection method in vivo and in vitro (100% vs. 89% and 100% vs, 78% respectively). Biopsy method by punching was significantly (p<0.05) higher than bisection between produced embryos in vivo and in vitro. The present data indicate that with microblade after punching for embryo sexing results in high incidence of survivability on development after embryo biopsy. It is also suggested that LAMP-based embryo sexing suitable for field applications.

This research was investigated the relationship between the number of the transferable embryos and estrus expression rate, BCS (Body Condition Score), which affect the nutritional state of the cow, in Holstein donor cows. CIDRs were inserted into the vaginas of twenty two head of Holstein cows, regardless of estrous cycle. Superovulation was induced using folliclar stimulating hormone (FSH). For artificial insemination, donor cows were injected with and estrus was checked about 48 hours after the injection. Then they were treated with 4 straws of semen 3 times, with 12-hour intervals. Embryos were collected by a non-surgical method 7 days after the first artificial insemination. When BCS was 2.5, the total number of collected ova was 7.3 + 1.9, which is significantly lower (p<0.05) than the numbers 15.4 + 2.8 and 15.4 + 2.1 that were obtained when BCSs were 2.75 and 3.0, respectively. Whereas the numbers of transferable embryos were 5.2 + 1.4 when BCS was 2.5, which was smaller than the numbers 6.0 + 2.1 and 8.5 + 1.8 that were obtained when BCSs were 2.75 and 3.0, respectively; however, the differences were not significant. As for estrus induction rate, the cow groups whose BCSs were 2.75 and 3.0 showed 100.0% and 95.0%, respectively. Whereas the cow group whose BCS was 2.5 showed 57.1%, and the differences were significant (p< 0.05). As for estrous expression rate, the cow groups whose BCSs were 2.5, 2.75 and 3.0 showed 100.0%, 100.0% and 85.7%, respectively; however, the differences were not significant. According to the result of this research, it is considered that the total number of collected ova and the number of transferable embryos will be affected by the nutritional state before and after in vivo embryo production and superovulation treatment, and that although the mechanism is not clear, poor stockbreeding management and nutritional level would cause the decrease of ovum recovery rate and the number of transferable embryos in high-producing cows. On the other hand, diverse researches on the superovulation treatment method that is suitable for high-producing Holstein donor cows would contribute to preventing ovarian cyclicity disorder, as well as to the early multiplication of cows with superior genes by increasing the utilization value of donor cows.

This research was investigated the relationship, in high-producing Holstein donor cows, between the number of the transferable embryos and the blood serum concentrations of Blood Urea Nitrogen (BUN), glucose and cholesterol, which affect the nutritional state of cows. CIDRs were inserted into the vaginas of twenty two heads of Holstein cows, regardless of estrous cycle. Superovulation was induced using folliclar stimulating hormone (FSH). For artificial insemination, donor cows were injected with and estrus was checked about 48 hours after the injection. Then they were treated with 4 straws of semen 3 times, with 12-hour intervals. Embryos were collected by a non-surgical method 7 days after the first artificial insemination. The total numbers of ova collected from 3 experimental groups whose blood BUN concentrations were <10 mg/dl, 11~18 mg/dl and mg/dl were 8.9, 12.5 and 19.0, respectively; whereas the numbers of transferable embryos were 5.8 + 1.9, 7.9 + 2.8 and 5.2 + 1.4, respectively. When glucose concentration was <60 mg/dl, the total number of collected ova was 9.9, which was smaller than when the concentration was 60~70 mg/dl or mg/dl. When glucose concentration was 60~70 mg/dl, the number of transferable embryos was 7.1 + 2.4, which was slightly larger than the numbers 6.4 + 2.1 and 6.1 + 1.7 that were obtained when the concentrations were <60 mg/dl and mg/dl, respectively ; however, the differences were not significant (p>0.05). When cholesterol concentrations were <150 mg/dl, 150~200 mg/dl and mg/dl, the total numbers of collected ova were 11.2, 11.3 and 8.6, respectively. Whereas the numbers of transferable embryos were 7.1 + 2.1, 7.3 + 1.9 and 5.6 + 1.3, respectively ; however, the differences were again not significant (p>0.05). The result of this research showed no significant difference in ovum recovery rate and the number of transferable embryos according to major metabolite concentrations in high-producing Holstein donor cows. However, it is considered that the failure of maintaining proper nutritional status would cause the fall in in vivo embryo productivity.

본 연구는 스웨덴 스톡홀름에서 색채경관의 변화에 대한 원인 분석 및 변화과정을 제시해본다. 사실 색채는 주변환경에 대해서 영향을 받는다. 스톡홀름은 도시주변에 물이 많기 때문에 내륙보다 색채의 변화가 많다. 보통 색채는 역사지구,상업지구, 주거지구에 따라 색채계획이 다르고 빛의 반사량에 따라 시시각각 다른 이미지도 갖는다.계절별 빛의 반사량에 따라 변하는 색상, 명도, 채도값을 측정해서 팔레트를 제시 분석하고, 파사드별로 지붕과벽체별로 빛에 따라 변화하는 색상값을 측정하였다. 계절별 시각적인 큰 변화도 물이 많은 도시에서 도시경관의 변화의차이가 큰 폭으로 나타난다. 거리에서의 색채 사용에 대한 평가는 색상, 명도, 채도값을 고려함은 물론이고 빛의 반사량도고려되어져야 한다. 이때 각 색채의 특성 및 인간의 시각적인 요인에 의한 효과도 심리적인 측면과 감정적인 측면이 동시에 분석되어져야 한다. 색채의 경향은 어느 장소에 있어서 건축물의 지붕, 벽과 도로의 아스팔트 그리고 거리의녹지공간에 따라 도시경관은 색채의 구성과 재료에 의해 분류된다.계절별로 건축물의 지붕과 벽면의 색상이 빛의 반사량에 따라 변화하는 색상값을 색차계를 이용해서 측정하였고그 결과는 먼셀기호로 표기하였다. 또한 그 값은 색채이미지맵에 색상값을 표시하여 죠닝화해보고 변화하는 경향을축으로 표현하였다. 마감 재료별, 입면 부분별로 구분해서 계절별 색상값을 추출한 후 칼라이미지맵에 빛의 반사량에따라 변화하는 색상값을 계절별로 변화하는 축을 만들어 모델화하였다.따라서 각 계절별로 건축물의 지붕, 벽, 보도패턴에 대한 색채경향 및 마감재료 사용 경향을 알 수 있었고 비와 눈,구름의 양에 따라 빛의 반사량이 달라지기에 색상의 변화과정도 비교 분석해 볼 수 있었다. 또한 물리적인, 심리적인 고려뿐만아니라 물과 녹지공간의 양에 따라서도 색상값이 변화하는 계절효과도 있었다는 특징을 분석해서 색채경향을 정리하였다.

Studies have shown that the future of the wine market rests on the effective and efficient changes in technology to the supply chain used by most of the major global players. In today’s wine industry, companies are faced with the ever-shifting demand for their products, strict regulation and increasing price competition. Even at that, mature companies in the wine industry are succeeding by scaling up production, streamlining their supply chains, expanding into new geographic areas, implementing more efficient processes, cleverly marketing products, and focusing on ever closer relationships with suppliers, partners and customers. However, this paper looks at supply chain challenges in the wine industry from a global perspective presented in the inbound, manufacturing and outbound processes as well as offer effective solutions in order for companies to gain a competitive advantage and succeed on a global level.

Research and technology has been transforming the agriculture to agribusiness which encompasses all operations with all the connections from faming per se, to manufacture & distribution of production supplies and farm commodities. Further, with the revolutionary development of information technology in the last two decades, we cannot talk about agribusiness process alone without considering the information technology embedded in the artifact, process, and structure. Despite the emergence of precision agriculture (PA) which is supported by IT based innovations which can not only improve efficiency in farming operations but also contribute to environmental sustainability, the adoption of IT among farmers and in agriculture industry are rather low than expected. Thus, Korean government has been seeking to converge IT into food, agriculture, forestry and fisheries to improve the competency of the agribusiness, and much progress has been made. This paper investigated the status quo of the current IT convergence with Food, Agriculture, Forestry and Fisheries in Korea, and further proposed future policy directions.

Since the Free Trade Agreements (FTAs) with Chile, the EU, and the U.S., Korean agricultural produce markets have turned into a fierce competition landscape. Under these competitive circumstances, marketing is critical. The objective of the research presented herein is to understand the characteristics of customer preferences after locating trends of purchased items. So This research establishes sustainable strategies for Korean agricultural produce. This investigation used market-basket analysis techniques and panel data for its research. Market-basket analysis is a technique which attempts to find groups of items that are commonly found together. The results show that, for one year, processed food using wheat, processed marine products, and pork are commonly bought together and that yogurt and milk also are bought together. The characteristics of customers buying these items are 44 years old and live in a four-person household with two children. These customers do not live with their parents.

As the ASEAN moves towards its vision of a ‘Community,’enforceability and consistency of legal standards, broadly the “rule of law,”have drawn attention due to their impact on the predictability of social environments, with consequences for markets, people, and policy makers. This paper draws together recent findings and suggests ASEAN States have made significant progress but remain in a state of transition. These findings support Barry Weingast’s prediction that developing countries are more likely to create consistent rules and move to“ open access orders” in line with requirements for development, rather than install artificial enforcement mechanisms before growth.