The objectives of the present study were to select an effective basic medium including its hormone and protein supplementation for IVM of oocytes of indigenous zebu cows. The ovaries of cows were collected from slaughter house and the follicular fluid was aspirated from 2 to 8 mm diameter follicles. The COCs with more than 3 cumulus cell layers and homogenous cytoplasm were selected for maturation. The oocytes were matured in media for 24 hrs at 39℃ with 5% CO2 in humidified air. The maturation of oocytes was evaluated by examining the presence of first polar body under microscope. An efficient basic medium was determined after culturing COCs in either TCM 199 or SOF medium in Experiment 1. An efficient hormone supplementation was determined after culturing COCs in either FSH or gonadotrophin supplemented TCM 199 in Experiment 2. An efficient protein supplementation was determined after culturing COCs in either FBS or Oestrous cow serum (OCS) supplemented TCM 199 in Experiment 3. The oocyte recovery rate per ovary was 3.35. The overall rate of IVM was 74.6%. The maturation rate was 75.5±3.9 and 62.2±20.2% in TCM and SOF medium, respectively (P>0.05). The maturation rate of oocytes was significantly higher (76.6±13.2%) in FSH supplemented medium than gonadotrphin supplemented counterpart (69.7±10.8%) (P<0.05). The maturation rates of oocytes were 81.7±12.9 and 85.7±12.7% in medium supplemented with FBS and OCS, respectively (P>0.05). In conclusions, both TCM 199 and SOF supplemented with either FBS or OCS, and FSH may be used as medium for IVM of indigenous zebu oocytes in Bangladesh.

Xenotransplantation of pig islet regarded as a good alternative to allotransplantation. However, cellular death mediated by hypoxia-reoxygenation injury after transplantation disturb success of this technique. In the present study, we produce transgenic pig expressing human heme oxygenase 1 (HO1) genes to overcome cellular death for improving efficiency of islet xenotransplantation. Particularly, Korean miniature pig breed, Micro-Pig, was used in the present study. Somatic cell nuclear transfer (SCNT) technique was used to produce the HO1 transgenic pig. Six alive transgenic piglets were produced and all the transgenic pigs were founded to have transgene in their genomic DNA and the gene was expressed in all tested organs. Also, in vitro cultured fibroblasts derived from the HO1 transgenic pig showed low reactive oxygen species level, improved cell viability and reduced apoptosis level

6-Gingerol exerts anti-tumor effects in various cancer cell models. We evaluated the effect of 6-gingerol on the growth of MCF-7 breast cancer cells and MCF-10A breast epithelial cells to determine whether any growth-inhibitory effects found were attributable to apoptosis, and to elucidate the underlying mechanism of action. 6-Gingerol inhibited the viability of both cell lines in a dose- and time-dependent manner; however, the degree of inhibition was greater in MCF-7 than MCF-10A cells. By flow cytometry, induction of dose- and time-dependent apoptosis was found, and the magnitude of apoptosis was also markedly greater in MCF-7 than MCF-10A cells. Expression of caspase-3 and poly (ADP-ribose) polymerase (PARP) was observed in MCF-7 cells treated with 6-gingerol, and further cleavage of PARP occurred in these cells. We suggest that 6-gingerol induces apoptosis in human breast cancer cells mainly by promoting caspase-3 expression and subsequent degradation of PARP.

To overcome the hyperacute immune rejection during pig-to-non-human primates xenotranasplantation, we have produced and bred α-1,3-galactosyltransferase knock-out (GalT —/—) pigs. In this study, the somatic cells and tissues from the GalT —/— pigs were characterized by an analysis of the expression of Galα-1,3-Gal (α-Gal) epitope. Briefly, ear fibroblast cell lines of 19 homozygous GalT —/— pigs were established and cryopreserved. The expression of α-Gal epitope in the cells was measured by fluorescence activated cell sorter (FACS) analysis using BS-I-B4 lectin. Also, the homozygous (GalT —/—) cells and tissues samples were immunostained with BS-I-B4 lectin for analysis of α-Gal epitope expression. The results showed that the expression of α-Gal epitope in GalT —/— cells (0.2 %) were significantly (p< 0.05) down-regulated to the range of cynomolgus monkey fibroblast (0.2 %) cells compared to heterozygous (GalT —/+) (9.3 %) and wild type (GalT +/+) (93.7 %) fibroblast cells. In the immunostaining results, while the expression of α-Gal epitope was detected a partly in GalT —/+ cells and mostly in GalT +/+ cells, it was almost not detected in the GalT —/— cells. Also, immunostaining results from various tissues of the GalT —/— pig showed that the expression of α-Gal epitope was not detectable, whereas various tissues from GalT +/+ pig showed a strong expression of α-Gal epitope. Our results demonstrated that α-Gal epitope expressions from GalT —/— pigs were successfully knocked out to prevent hyperacute immune rejection for further study of xenotransplantation.

농촌사회의 소득수준 및 생활수준 향상과 더불어 농촌 공공공간 및 시설향상에 대한 요구는 지속적으로 제기되어 왔으며 이에따라 정부도 최근 다양한 방식의 농촌개발과 예산지원을 추진해오고 있다. 그러나 이러한 농촌 공공공간 및 시설의 지원 시 시설의 종류 및 입지 선정이 적절하지 않아 시설의 중복투자, 이용부족으로 인한 시설방치 등 다양한 문제점들이 제기되고 있다. 본 연구는 농촌마을에 분포하고 있는 다양한 공공공간 및 시설의 분포를 분석하고 이들에 대한 농촌마을의 유형별 중요도를 이해하는데 그 목적이 있다. 주요 연구방법으로는 100명을 대상으로 한 전문가 설문과 AHP(Analytic Hierarchy Process)를 이용하였으며 이를 통해 세 개의 농촌마을 유형 즉 거점면, 권역, 마을단위의 유형별로 주요 공공시설들간의 상대적 중요도를 분석하였다. 50개 마을을 분석한 결과 농촌마을에서 가장 일반적으로 발견되는 공공시설은 마을회관, 쉼터, 체험센터, 마을진입부, 안내시설로 나타났다. AHP는 세 단계의 비교로 이루어졌는데 첫 번째 마을유형별간의 쌍대비교에서는 거점면, 권역, 마을 순으로 중요도가 나타났다. 농촌 공공공간과 마을유형간의 쌍대비교에서는 모든 마을유형에서 기초생활시설, 문화 복지시설, 농촌관광시설, 소득시설의 순으로 중요도가 나타났다. 마을유형과 공공공간, 공공시설 등 각각의 가중치를 종 합한 중요도에서는 거점면의 경우 공동주차장의 중요성이 가장 높은 것으로 나타났고 다음으로 상수도시설, 공동화장실, 마을회관 순으로 나타났다. 권역에서는 공동주차장의 중요성이 가장 높았으며 다음으로 상수도시설, 체험센터, 마을회관 의 순으로 나타났다. 마을단위에서는 버스정류장이 가장 중요한 시설로 평가되었으며 다음으로 저수지, 하수처리장, 체 험센터의 순으로 나타났다. 본 연구는 비록 마을의 독특한 사회문화적 환경을 고려한 공공시설의 중요도 평가에는 한계를 안고 있으나 농촌마을에서의 공공시설 계획 시 마을유형의 특성을 고려한 시설의 상대적 중요도를 이해하고 이를 바탕으 로 적절한 시설을 선정하는데 도움을 줄 것으로 기대된다

Poria cocos is an edible and pharmaceutical mushroom with a long history of medicinal use in Korea. For the last 30 years, the domestic cultivated supply of Poria cocos has been unable to meet consumer demand, so Poria cocos is collected in mountainous areas and also imported from China. Thus, to increase the supply of Poria cocos, many artificial cultivation methods have been studied. In this study, Poria cocos is cultivated under different environmental conditions using plastic bags and the results compared. When cultivating Poria cocos at different temperatures (20, 25, 30 and 35oC) and using different numbers of plastic bag layers (1, 2), the most efficient cultivation conditions were a temperature of 25-30oC and 2 plastic bag layers. The fastest growth was at 25-30oC, and the Poria cocos exhibited no weight change when cultivated using layers of plastic bags (1, 2).

산업화 시대 이후 공간설계에 있어서 생태적 접근은 지속적으로 주목 받아왔다. 옥외환경디자인의 영역, 특히 조경설 계에 있어서 생태적 설계는 크게 두 가지의 흐름으로 나뉜다고 볼 수 있다. 첫째는 기술적인 측면의 생태설계로서 설계 행위를 통하여 환경의 생태적 건강성을 향상시키는데 집중하는 것을 강조하는 분야이며 둘째는 생태적인 공간을 사회문 화적으로 이해하고 어떻게 대중에게 그 공간의 생태적 가치를 설계라는 매개를 통해 전달할 것인가를 고민하는 분야이 다. 본 연구에서는 후자의 관점에서 조경설계가 공간의 어떤 주제를 어떠한 방법으로 다룰 때 생태적 의미가 더 효과적 으로 전달될 것인가에 대한 고찰을 하였다. 따라서 생태적 조경설계의 여섯 가지 대상을 요약 및 분석하고 그에 따른 설계언어 여섯 가지를 제안하여 논증하였다. 설계주제로는 생태적 기능, 경관의 일시성, 역사성, 시각적 흥미, 안전성, 접근성을 요약하였고 설계언어는 구체적으로 설명, 상징, 대비, 틀, 다양화, 그리고 거리두기를 제안하고 논의하였다. 본 연구의 의미는 산재되어 있는 설계언어를 종합하여 구체적이고 실질적으로 어떻게 생태설계에 응용될 수 있는가에 대하여 제안하고 각 설계언어에 대한 실증적 연구에 기초를 마련하는 데 기여하는 것이다.

한국산 딱부리반날개속(Stenus Latreille)의 8종에 대해 보고한다. 이들 중, 3종(Stenus burjaetus Puthz, S. depressus Puthz and S. gibbicollis J. Sahlberg)은 한반도산 미기록종이고, 나머지 5종은(S. coronatus coronatus L. Benick, S. decoratus L. Benick, S. koreanus Puthz, S. rugipennis Sharp and S. sharpi Bernhauer and Schubert)은 남한에서 처음 보고되는 종들이다. 8종의 성충 사진, 수컷의 생식기 및 9번째 복판에 대한 그림과 한국산 딱부리반날개속의 종 검색표를 제공한다.

Using reverse micelle processing, ZnAl2O4 nanopowders were synthesized from a mixed precursor(consisting of Zn(NO3)2 and Al(NO3)3). The ZnAl2O4 was prepared by mixing the aqueous solution at a molar ratio of Zn : Al = 1 : 2. The average size and distribution of the synthesized powders with heat treatment at 600 oC for 2 h were in the range of 10-20 nm and narrow, respectively. The average size of the synthesized powders increased with increasing water to surfactant molar ratio. The XRD diffraction patterns show that the phase of ZnAl2O4 was spinel(JCPDS No. 05-0669). The synthesized and calcined powders were characterized using a thermogravimetric - differential scanning calorimeter(TG-DSC), X-ray diffraction analysis (XRD), and high resolution transmission electron microscopy(HRTEM). The effects of the synthesis parameter, such as the molar ratio of water to surfactant, are discussed.

In this article, poly methyl triethoxy silane was compounded with an inorganic waterproof admixture at a certain ratio to improve the performance of gypsum products; a new type of high-efficiency compound water-proofing additive was also investigated. Furthermore, the waterproof mechanism and the various properties on the hardened gypsum plaster were investigated in detail by XRD and SEM. The results show that the intenerate coefficient of gypsum plaster increased to more than 0.9; the water absorbing rate decreased to less than 10 %. Both the bending strength and the compressive strength of gypsum plaster increased by various degrees. The intenerate coefficient reached a maximum value of 0.73 and the strength of the samples showed almost no change when 5 % cement alone was added. In this new type of the high-efficiency compound with waterproof additive, the optimal technological parameters for formulas were obtained to be: 5 % cement, 18 % mineral powder, and 0.8 % poly methyl triethoxy silane, to compound gypsum plaster. Meanwhile, the production of high performance gypsum as a building material has become possible.

Bee pollen is rich in various nutrients and called as natural complete food. Bee pollens of acorn (Quercus acutissima) and actinidia (Actinidia arguta) are the most collected in Korea. But stiff pollen wall hinders dissolution of polysaccharides and lowers extraction efficiency. Thus, we measured antioxidant activity as DPPH radical scavenging and the total polyphenol content of pulverized and lyophilized actinidia pollens inoculated with fungi to confirm the husk removal effect. The total polyphenol content of actinidia pollen was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in actinidia pollen germinated with A. mellea. The total polyphenol content of the lyophilized actinidia pollen germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH (2, 2 diphenyl-1-picrylhydrazyl) free radical scavenging method showed that the lyophilized actinidia pollen germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized actinidia pollen germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of actinidia pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from actinidia pollen inoculated with A. mellea.

Oyster mushroom is one of mushrooms that are cultivated and consumed a lot in Korea. P. ostreatus 'Suhan(ASI2504)' is a popluar cultivar because of high quality. But it is difficult for farmers to cultivate, so an alternative cultivar of ‘Suhan’ is demanded continuously. To develop a new variety, parental strains were selected using cultivation characteristics of Pleurotus ostreatus collected home and abroad. P. ostreatus 'Gosol' was developed by the method of Di-Mon crossing between dikaryotic strain P. ostreatus 'Mongdol(ASI0633)' and monokaryotic strain derived from P. ostreatus ‘Yasan(ASI0635)' in 2014. Analysis of the genetic characteristics of the new cultivar 'Gosol' showed a different DNA profile as that of the control strain, 'Suhan’ but a similar DNA profile as those of the parental strains, ‘Yasan’ and　'Mongdol’, when RAPD(Random Amplified Polymorphic DNA) primer URP 3 was used. The optimum temperature for mycelial growth was 25°C for ‘Gosol’ and ‘Suhan’. 'Gosol' was appropriate for middle high temperature to grow, especially 13~18°C. Fruiting body production per bottle was about 124.2g. When compared to the control strain 'Suhan’, the stipe was longer and the individual weight was heavier. But the stipe and the pileus were a little thinner than those of the control strain. ‘Gosol’ was more resistant at high CO2 concentration than the control strain. This new cultivar ‘Gosol’ of Pleurotus ostreatus was characterized dark bluish gray cultivar of oyster mushroom in the color of pileus and higer yield compared to those of other cultivar ‘Suhan’. Therefore, it is suggested that this new cultivar ‘Gosol’ be substitute for ‘Suhan’ in oyster mushroom’s farms.

The aim of this study was to analyze the genetic diversity of collected strains of Hypsizygus marmoreus based on their rDNA ITS sequences. The size of ITS rDNA regions of H. marmoreus strains, collected form various regions. A phylogenetic trees based on the ITS region revealed that the strains could be classified into 4 different groups including Villosiclava virens, Hypsizygus marmoreus, Lepista irina, Lyophyllum Decastes, Lyophyllum shimeji, Pleurotus floridanus.

The aims of the current study were to assess reliability of range of motion (ROM) measurement of glenohumeral internal rotation (GIR) with a pressure biofeedback stabilization (PBS) method and to compare the reliability between manual stabilization (MS) and the PBS method. In measurement of pure glenohumeral joint motion, scapular stabilization is necessary. The MS method in GIR ROM measurement was used to restrict scapular motion by pressing the palm of the tester’s hand over the subject’s clavicle, coracoid process, and humeral head. The PBS method was devised to maintain consistent pressure for scapular stabilization during GIR ROM measurement by using a pressure biofeedback unit. GIR ROM was measured by 2 different stabilization methods in 32 subjects with GIR deficit using a smartphone clinometer application. Repeated measurements were performed in two test sessions by two testers to confirm inter- and intra-rater reliability. After tester A performed measurements in test session 1, tester B’s measurements were conducted one hour later on the same day to assess the inter-rater reliability and then tester A performed again measurements in test session 2 for confirming the intra-rater reliability. Intra-class correlation coefficient (ICC) (2,1) was applied to assess the inter-rater reliability and ICC (3,1) was applied to determine the intra-rater reliability of the two methods. In the PBS method, the intra-rater reliability was excellent (ICC=.91) and the inter-rater reliability was good (ICC=.84). The inter-rater and intra-rater reliability of the PBS method was higher than in the MS method. The PBS method could regulate manual scapular stabilization pressure in inter- and intra-rater measuring GIR ROM. Results of the current study recommend that the PBS method can provide reliable measurement data on GIR ROM.

This study compared the effects of the initial head position (i.e., a HHP versus a relaxed head position) of subjects with and without a FHP on the thickness of the deep and superficial neck flexor muscles during CCF. The study recruited 6 subjects with a FHP and 10 subjects without a FHP. The subjects performed CCF in two different head positions: a HHP, with the head aligned so that the forehead and chin formed a horizontal line, and a relaxed head position (RHP), with the head aligned in a self-selected comfortable position. During the CCF exercise, the thickness of the longus colli (LCo) and the thickness of the sternocleidomastoid (SCM) were recorded using ultrasonography. The thickness of each muscle was measured by Image J software. The statistical analysis was performed with a two-way mixed-model analysis of variance. The thickness of the SCM differed significantly (p<.05) between the subjects with and without FHP. According to a post hoc independent t-test, the change in thickness of the SCM increased significantly during CCF in the subjects with FHP while adopting a HHP compared to that in the subjects without FHP. The change in thickness of the SCM was not significantly different between the two positions in subjects without FHP, and there was no significant change in thickness of the LCo muscle during the CCF exercise according to the initial position in both subjects with and without FHP. The results suggest that CCF should be performed in RHP to minimize contraction of the SCM in subjects with a FHP.