This research investigated the effects of the plant growth regulators (PGRs) on fruit characteristics and storability of ‘Shine Muscat’ grapes. The objective was to identify effective PGR treatment methods for high-quality production and efficient storage. The characteristics of ‘Shine Muscat’ fruits were more affected by the secondary treatment applied 14 days after full bloom than by the primary treatment applied at full bloom. The fruits treated with thidiazuron (TDZ) in the secondary treatment showed higher weight. However, the forchlorfenuron (CPPU) treated fruits exhibited lower acidity and higher soluble solids content, which led to a higher sugar-to-acid ratio. Additionally, it had higher firmness, contributing to a good texture and a brighter yellow-green skin color, thus indicating better quality. The storage period for ‘Shine Muscat’ varies depending on the storage method: up to 60 days in cold storage and up to 110 days in Controlled Atmosphere (CA) storage. Like other kinds of fruits, CA storage was more effective for long-term storage. Furthermore, considering that the quality characteristics manifested during the prestorage PGR treatment were maintained during CA storage, secondary treatment with CPPU was deemed effective for optimal quality and storage.

In the post-COVID-19, the food industry is rapidly reshaping its market structure toward online distribution. Rapid delivery system driven by large distribution platforms has ushered in an era of online distribution of fresh seafood that was previously limited. This study surveyed 1,000 consumers nationwide to determine their online seafood purchasing behaviors. The research methodology used factor analysis of consumer lifestyle and Heckman’s ordered probit sample-selection model. The main results of the analysis are as follows. First, quality, freshness, selling price, product reviews from other buyers, and convenience are particularly important considerations when consumers purchase seafood from online shopping. Second, online retailers and the government must prepare measures to expand seafood consumption by considering household characteristics and consumer lifestyles. Third, it was analyzed that consumers trust the quality and safety of seafood distributed online platforms. It is not possible to provide purchase incentives to consumers who consider value consumption important, so improvement measures are needed. The results of this study are expected to provide implications on consumer preferences to online platforms, seafood companies, and producers, and can be used to establish future marketing strategies.

목적 : 본 연구는 국민건강영양조사 제8기(2019-2021) 원시자료를 이용하여, 생체지표를 통한 휘발성 실내환 경요인(휘발성 유기 화합물, VOCs)과 백내장의 연관성을 파악하고자 하였다. 방법 : 백내장 의사진단 여부 및 가정 실내공기질 측정에 참여한 만 40세 이상의 성인 총 1,150명을 대상으로 하였다. 일반적 특성에 따른 휘발성 실내환경요인의 농도와 백내장의 유 ‧무에 따른 휘발성 실내환경요인의 농도를 비교하기 위해 복합표본 기술통계 분석과 로지스틱회귀분석을 하였다. p<0.050인 경우 유의한 것으로 판단하였다. 결과 : 대부분의 실내 휘발성 환경오염물질의 생체지표는 백내장을 진단받은 대상자에서 높게 나타났다. 특히 Benzene, Xylene, Acrolein, 1-Bromopropane, 1,3-Butadiene의 생체지표 평균농도(GM)가 백내장 진단받은 대상자들이 유의하게 높게 나타났다. 연령, 성별, 결혼, 알콜, 흡연, 소득을 보정한 복합표본 로지스틱회귀분석에 서, 1,3-Butadiene의 생체지표는 약 2배(OR 1.905(95% CI: 1.001, 3.625))의 위험도로 백내장에 영향을 미칠 수 있는 것으로 나타났다. 결론 : 본 연구는 기존에 밝혀지지 않은 휘발성 실내환경요인과 백내장의 연관성을 파악함으로써, 일부 휘발성 실내환경물질은 백내장의 원인물질로 작용할 수 있는 가능성을 보여주었다.

Background: The biological information of fish, which include reproduction, is the prerequisite and the basis for the assessment of fisheries. Methods: The aim of this work was to know the reproductive biology with the first sexual maturity (TL50) and the spawning period for 58 mainly fish species in the waters around La Réunion Island (Western Indian Ocean). Twenty families belonging to the Actinopterygii were represented (acanthuridae, berycidae, bramidae, carangidae, cirrhitidae, gempylidae, holocentridae, kyphosidae, labridae, lethrinidae, lutjanidae, malacanthidae, monacanthidae, mullidae, polymixiidae, pomacentridae, scaridae, scorpaenidae, serranidae, sparidae; 56 species; n = 9,751) and two families belonging to the Elasmobranchii (squalidae, centrophoridae; 2 species; n = 781) were sampled. Between 2014 and 2022, 10,532 individuals were sampled covering the maximum months number to follow the reproduction periods of these species. Results: TL50 for the males and the females, respectively, ranged from 103.9 cm (Acanthurus triostegus ) to 1,119.3 cm (Thyrsitoides marleyi ) and from 111.7 cm (A. triostegus ) to 613.1 cm (Centrophorus moluccensis ). The reproduction period could be very different between the species from the very tight peak to a large peak covered all months. Conclusions: Most species breed between October and March but it was not the trend for all species around La Réunion Island.

Plastics are widely used in industries in human society and because of their structural stability, degradation is a serious global issue. To estimate the degradation of plastic, 31 edible mushrooms were cultured with the selected plastic films (polyethylene [PE], polystyrene [PS], and poly(ethylene terephthalate) [PET]) for 3 months at 25 °C. Measuring the weight of the films showed that four species of mushrooms, namely Porostereum spadiceum, Ganoderma lucidum, Coprinellus micaceus, and Pleurotus ostreatus, exhibited the highest degrees of plastic degradation. In addition, the mushrooms and fungi that exhibited the most significant plastic degradation were cross-cultured to promote this degradation. As a result, cross-cultivation of G. lucidum and Aspergillus niger showed a weight loss of 2.49% for the PET film. For the PS film, Aspergillus nidulans showed a weight loss of 4.06%. Cross-cultivation of A. nidulans and C. micaceus, which showed a weight loss of 2.95%, was noted as an alternative for PS biodegradation, but is harmful to humans. These bio-degradation effects of edible mushroom will contribute to the development of alternatives for eco-friendly plastic degradation.

The current survey form of grassland has not played its role in managing the grassland effectively because of the ambiguous questionnaire items and the absence of method and time of the investigation. Therefore, this study was conducted to clarify and add the items for effective of grassland management. The survey form of grassland was regulated in Article 16 of the Enforcement Rules of the Grassland Act (Survey on Grasslands Management Status, etc.). The five contents that needed improvement were the grassland owner, the survey timing and method of established grassland, grassland used livestock, grassland usage condition, and grassland grade. In the improved survey form of grassland, the grassland owner was changed to the grassland manager. In addition, ‘Survey by each land’ was added to the improved survey form in which the managers can survey each land. The dimension of each grassland establishment method was deleted in the grassland establishment time and method. In the livestock using grassland, the number of livestock and the area where livestock are used were added, and the number of other livestock was added, too. The grassland grade was decided as the combined score by three evaluation categories; grassland usage, the ratio of forage production in grassland and kinds of forage. The high, middle, and low grades were over 8, 6~7, and under 5 points in the combined score, respectively. The results show that the changed survey form of grassland can make grassland management more efficient by materializing the subject of grassland management and the survey terminology and methods.

Bolivian tuber species like potato (Solanum tuberosum), native potato (Solanum sp), Oca (Oxalis tuberosa Molina), Olluco (Ullucus tuberosus Caldas), and Isaño (Tropaeolum turosum Ruíz & Pav.) hold extraordinary nutritional value and cultural significance, particularly within the Andean region. This study examined the mineral composition of Bolivian tuber species as an essential step toward understanding their nutritional significance and potential contributions to addressing dietary deficiencies. The research involved detailed analysis of diverse tuber cultivars, uncovering distinct mineral profiles across species. Native potato shows high levels of nitrogen (N), potassium (K), phosphorus (P), and magnesium (Mg) levels, alongside moderate micronutrients like iron (Fe) and zinc (Zn). Commercial potatoes exhibited prominence in N, P, and K, with moderate Fe, Zn, and manganese (Mn) levels. Oca, Isaño, and Papa Lisa displayed unique mineral concentrations, offering potential nutritional benefits. Intricate correlations and significant variances among elements highlighted the diverse mineral compositions among these tuber species. Multivariate analyses emphasized distinct mineral profiles unique to each species, revealing significant compositions of isaño and papa lisa's. The Multitrait Genotype- Ideotype Distance Index (MGIDI) identified isaño jaspeado, isaño and an unnamed native potato, AXT2, as promising ideotypes due to their exceptional mineral compositions. These findings provide comprehensive insights into Bolivian tuber species' various mineral compositions, underscoring their nutritional significance and potential in targeted breeding for improved dietary support and enhanced food security.

In this study, in order to analyze the water purification efficiency according to the influent water conditions of artificial wetlands, the purification efficiency was compared at two points where sewage treatment water flows in and one point where good effluent flows in. As a result of reviewing the results of the analysis of influent and effluent and the removal efficiency, the T-N and T-P removal efficiency was calculated at 54.7% and 77.4%, respectively, for the two points where sewage treatment water was treated, the treatment efficiency of SS 90.8%, BOD 51.1%, TOC 30.6%, T-N 38.8%, T-P 55.3% was shown. As a result, the efficiency of removing pollutants in the artificial wetland was found to be proportional to the concentration of influent water, and in order to create an efficient artificial wetland, it is judged that thorough review and management at the design stage are necessary considering that the removal efficiency of high-concentration contaminated water was high.

As a member of ectomycorrhizal fungi, Tricholoma matsutake has a symbiotic relationship with its host, Pinus densiflora. To cultivate T. matsutake artificially, the co-cultivation of T. matsutake mycelia and bacteria from shiro was introduced. In this study, bacteria were isolated from soil samples in Bonghwa-gun, and seven bacterial isolates (B22_7_B05, B22_7_B06, B22_7_B07, B22_7_B08, B22_7_B10, B22_7_B13, and B22_7_B14) promoted the growth of T. matsutake mycelia (147.48, 232.11, 266.72, 211.43, 175.17, 154.62, and 177.92%, respectively). Sequencing of the 16S rRNA region of the isolated bacteria was performed. B22_7_B05 and B22_7_B10 were identified as Bacillus toyonensis, B22_7_B06 and B22_7_B08 as Paenibacillus taichungensis, B22_7_B07 and B22_7_B14 as P. gorilla, and B22_7_B13 as P. odorifer. These bacterial isolates were associated with the shiro community and are expected to contribute to the cultivation of T. matsutake.

This study addresses the scarcity of research examining technology’s impact on learners of English as a foreign language in English for specific purposes (ESP) courses within English-medium instruction (EMI) programs. Focusing on a business communication course using a social question-answering platform, this study explores the interplay among English proficiency, motivational orientations, and online engagement. Surveying 126 university students via a mixed-method approach, this research identifies distinct motivational orientations (intrinsic, extrinsic, and self-efficacy) existing during class activities, influenced by proficiency levels, with notable differences in online participation between beginner and advanced learners. The findings suggest that while online activities enhance peer interaction and teacher feedback, they may trigger anxiety in beginners, potentially diminishing motivation and self-efficacy. These results emphasize the need to tailor technology integration to proficiency levels and motivational orientations in ESP/EMI settings. Practical recommendations are offered for educators aiming to implement effective online activities and provide targeted support, particularly for beginner learners, thereby bolstering their motivation and selfefficacy.

이 연구는, 생활종교(lived religion) 연구 방법을 사용하여, 한국의 대표적인 여성 작가이자 가톨릭 신자인 박완서와 공지영이 어떻게 그리스도교를 해석하고 그것을 문학적 실천에 반영했는지를 탐구한다. 박완서는 종교는 사람의 중심을 바로 잡아주는 것이라 보았고, 이에 따라 반성을 중요한 종교적 실천으로 보았다. 반면에, 공지영은 종교는 사람의 진실을 알아주는 것이라 보았고, 따라서 자기 용납을 중요한 종교적 실천으로 보았다. 이들은 종교에 대한 이러한 해석을 문학의 의미와도 연결했는데, 그 해석이 문학적 실천으로 드러난 대표적인 사례로 박완서의 ｢사람의 일기｣와 공지영의 󰡔우리들의 행복한 시간󰡕을 생활종교의 관점에서 분석하였다. 결과적으로 박완서의 생활종교는 유교와 그리스도교의 혼종 양상을 띠면서 자기를 반성하는 인간으로 표상되며, 공지영의 생활종교는 그리스도교와 치료 심리학의 혼종 양상을 띠면서 자기를 용납하는 인간으로 표상되었다. 이 연구는 20세기 말에서 21세기 초 한국 사회의 그리스도교 혼종 양상과 실천의 다양성 그리고 변화를 엿보게 해준다는 데에도 의의가 있다.

목적: 본 연구는 학습과정에서 보일 수 있는 심리적인 특성과 성격적인 요인을 진단할 수 있는 U&I 학습성격유형검 사를 이용하여 학습성격에 따른 시기능훈련 전·후 향상도를 알아보고자 하였다. 방법 : 안과질환 및 전신질환이 없는 성인 남녀 34명을 대상으로 학습성격유형을 분류하고 양안시검사 결과에 따라 적합한 시기능훈련 도구를 적합한 선정하여 시기능훈련을 실시하였다. 결과 : 학습성격유형과 시기능훈련을 상관 분석한 결과 행동형은 음성상대조절력과 양의 상관관계를 보였으며 규범형은 원거리 양성융합버전스 회복점과 양의 상관관계를 보였다. 탐구형은 양성상대조절력과 음의 상관관계를 보였고 이상형은 음성상대조절력과는 양의 상관관계, 근거리 양성융합버전스 흐린점은 음의 상관관계를 보였다. 학 습성격유형에 따른 시기능훈련 효과를 분석한 결과 융합용이성에서 규범형이 이상형보다 시기능훈련 전·후 융합 용이성의 효과가 유의하게 증가하였다. 결론 : 학습성격유형에 따른 시기능훈련 효과에 차이가 있음을 확인할 수 있었으며 그에 맞는 훈련법을 고안한 다면 더 체계적인 시기능훈련 방법을 적용할 수 있을 것으로 사료된다.

Tricholoma matsutake is a traditional favorite food in East Asia, cultivated in fairy rings called “shiro,” which are found near Pinus densiflora. For effective artificial cultivation of Tri. matsutake, microorganisms from symbiotic fairy rings are co-cultivated. In this study, one bacterial isolate (Y22_B35) and two fungal isolates (Y22_F64 and Y22_F68) displayed growth-promoting effects on Tri. matsutake mycelium (158.47, 125.00, and 122.26% enhanced growth, respectively). For identification, 16S rRNA or ITS regions from the microorganisms¡¯ genomes were sequenced. Other sequences, including BenA, CaM, and RPB2 were sequenced in the fungal isolates. The bacterial isolate Y22_B35 was identified as Bacillus cereus. Y22_F64 and Y22_F68 were identified as Umbelopsis nana and Aspergillus parvulus, respectively. To identify the effects of the dominant microorganisms on Tri. Matsutake cultivation, metagenomic analyses were performed. Discovery of these Tri. matsutake mycelium growth-promoting microorganisms and metagenomics analyses are expected to contribute to our understanding of Tri. matsutake fruiting body growth and construction of biomimicry.

In this study, we collected and analyzed the current status of licenses for the limited fishery business and divided the problems related to licenses for the limited fishery business into partial damage compensation and cancellation compensation areas. In the case of partial damage compensation areas due to existing public water use projects, it is suggested that the issuance of licenses for limited fishery businesses should be reconsidered. In the case of cancellation compensation areas, it is recommended that the disposition of communal fishery businesses that do not require capital investment should be the principle. If capital such as facilities are invested, compensation should be made by Article 52 of the Enforcement Decree of the Land Compensation Act if the licenses for limited fishery business are closed due to other development projects. In addition, we proposed an improvement plan to establish a rational management system for licenses for limited fishery businesses. In addition to these improvement measures, we hope that further investigation and research on licenses for limited fishery business, which have been insufficient so far, will be conducted to promote the comprehensive use of fisheries resources and waters and the sustainable development of fisheries that are the objectives of the Fisheries Act, and contribute to the improvement of the quality of life of the people and the balanced development of the national economy.

In this study, the expression of genes related to fruit ripening was investigated using realtime PCR. The study aimed to determine the effective conditions of calcium compounds (Calcium citrate, calcium chloride, calcium nitrate, GH-Ca, OS-Ca) and chitosan treatment to extend the shelf-life in the ‘Kumhong’ nectarine and ‘Madoka’ peach fruits. In this study, in the ‘Kumhong’ and ‘Madoka’ fruits, the expression of genes related to cell wall degradation, pectin lyase (PL), polygalacturonase (PG), and pectin methylesterase (PME), was inhibited by calcium, chitosan, or a mixture (Chitosan+CaCl2) treatment. In ‘Madoka’ peach, although the expression of the PG gene was suppressed at pre-harvest Ca and chitosan treatment, the expression of the PL gene was induced at pre- and post-harvest Ca and chitosan treatment compared to the control. The spray of calcium, chitosan itself, or a mixture (Chitosan+ CaCl2) on the trees at the ripening stage and harvested fruits can extend the shelf-life by suppressing the expression of cell wall degrading enzymes genes (PL, PG, PME) in ‘Kumhong’ nectarine and ‘Madoka’ peach tree. These results provide valuable information for the development of technology for the extension of the shelf-life of peach and nectarine fruits.

Given the recent increases in feed cost, the demand for rice straw has been increasing on dairy farms because it is cheaper than expensive imported forages. Therefore, it is necessary to study the effect of rice straw feeding on the growth and productivity of dairy heifers. This study compared how feeding tall fescue and rice straw affects the growth, age at first calving, and feed cost of heifers. we compared the growth, age at first calving, and feed cost for each group during the breeding period. Body height was higher in the tall fescue-fed group than in the rice straw-fed group (p<0.05). The length of the growing stage was significantly shorter in the tall fescue group than in the rice straw group (p<0.001) and the age at calving also showed a significant reduction in the tall fescue group compared to the rice straw group (p<0.001). However, the feed cost was lower in the rice straw group because of the difference in the unit price of forage (p<0.001). Based on these results, during the growing period, the rice straw group had delays in growth and the age at first calving compared to the tall fescue group, but also had reduced feed costs in the short term.

Background: Although an understanding of the proliferation and differentiation of fish female germline stem cells (GSCs) is very important, an appropriate threedimensional (3D) research model to study them is not well established. As a part of the development of stable 3D culture system for fish female GSCs, we conducted this study to establish a 3D aggregate culture system of ovarian cells in marine medaka, Oryzias dancena. Methods: Ovarian cells were separated by Percoll density gradient centrifugation and two different cell populations were cultured in suspension to form ovarian cell aggregates to find suitable cell populations for its formation. Ovarian cell aggregates formed from different cell populations were evaluated by histology and gene expression analyses. To evaluate the media supplements, ovarian cell aggregate culture was performed under different media conditions, and the morphology, viability, size, gene expression, histology, and E2 secretion of ovarian cell aggregates were analyzed. Results: Ovarian cell aggregates were able to be formed well under specific culture conditions that used ultra-low attachment 96 well plate, complete mESM2, and the cell populations from top to 50% layers after separation of ovarian cells. Moreover, they were able to maintain minimal ovarian function such as germ cell maintenance and E2 synthesis for a short period. Conclusions: We established basic conditions for the culture of O. dancena ovarian cell aggregates. Additional efforts will be required to further optimize the culture conditions so that the ovarian cell aggregates can retain the improved ovarian functions for a longer period of time.

Despite advancements in therapeutic approaches, radiotherapy and cisplatin-based chemotherapy remain primary noninvasive treatments for patients with oral squamous cell carcinoma (OSCC). Moreover, the 5-year survival rate for patients with OSCC has remained almost unchanged for several decades, and many side effects of chemotherapy still exist. In this study, three-dimensional (3D) models of OSCC were established using fibroblasts, and the efficacy of various biological inhibitors was evaluated. A culture of epithelial cells with two types of fibroblasts (hTERT-hNOFs and cancer-associated fibroblasts) within a type I collagen matrix resulted in the formation of a continuous layer of tightly packed cells compared to models without fibroblasts. Furthermore, the effects of biological chemicals, including Y27632, latrunculin A, and verteporfin, on these models were investigated. The stratified formation of the epithelial layer and invasion in OSCC 3D-culture models were effectively inhibited by verteporfin, whereas invasion was weakly inhibited by Y27632 and latrunculin. Collectively, the developed OSCC 3D-culture models established with fibroblasts demonstrated the potential for drug screening, with verteporfin showing promising efficacy.

This study investigated the fish fauna of Jeonjucheon and Samcheon Streams, flowing through the city of Jeonju, through surveys conducted at 10 sampling sites using kick net and cast net. The fish fauna collected included 10 families and 36 species, with a total of 2,064 individuals. Samcheon had 8 families and 30 species with 1,074 individuals, Jeonjucheon had 8 families and 26 species with 986 individuals. The dominant species was Zacco platypus with 1,202 individuals, and a total of 153 individuals of the subdominant were Pseudogobio esocinus. In Jeonjucheon Stream, a total of 567 individuals of the dominant species were collected as Z. platypus and 99 individuals of the subdominant species were collected as Pungtungia herzi, and there was no significant change in the stream environment except for the confluence site. Further expanded research covering the entire Jeonjucheon and Samcheon Streams region, accompanied by regular monitoring, is essential to record and understand fluctuations in fish fauna.

Mushroom-based vegan meat has thus far been used as a food for humans instead of pets. However, based on its texture and nutritional content, it is considered suitable for processing into pet treats. In the present study, we developed a prototype dog chew with a sweetening coating added to a fungal mycelium mat obtained by culturing the Basidiomycetous fungus Trametes orientalis. The palatable coating applied to the mycelium mat by plasticizing the mat with glycerol improved the taste and aroma of the existing mat, and the dog consumed it without difficulty. Future improvements may include a softening process to reduce the chewiness level and a procedure to reduce the crude fiber content. Mycelium-mat-based dog chews, manufactured using eco-friendly materials and processes that are not harmful to the environment are expected to enter the market as eco-friendly alternatives to conventional pet treats. Controlling their physical properties require further study.