Mucin coat is deposited on the embryos during passage through the oviduct in rabbit. When in vitro cultured blastocysts were transferred to the recipients, the lack of mucin coat might account in part for failure of pregnancy after transfer. The present study were carried out to investigate whether deposition of mucin coat were induced when in vitro cultured blastocysts were transferred to recipients. At 19 ～20 hours post-coitus one-cell embryos were collected by flushing oviducts. These embryos cultured for 72 hours were reached to blastocyst stage. And these blastocysts were transferred to the oviduct of asynchronized (one day later than the donors) and synchronized recipient. To confirm deposition of the mucin coat, blastocysts transferred to the oviduct were recovered at 24 and 48 hours after the transfer. Fifty eight percent of blastocysts recovered from uterus of asynchronous recipient at 24 hours after transfer and 92.9% of blastocysts recovered from uterus of synchronous recipient were 0～10 ㎛ of mucin coat thickness. And 11.8% of blastocysts of asynchronized recipients and 7.1% of blastocysts from asynchronized recipients were in 11～20 ㎛ of mucin coat thickness. When blastocysts were recovered from uterus at 48 hours after transfer, 87.0% of blastocysts from asynchronized recipients and 5.9% of blastocyst from synchronized recipients were in 0～10 ㎛ of mucin coat thickness. And 76.5% of blastocysts of synchronized recipients and 4.4% of blastocysts from asynchronized recipients were in 11～20 ㎛ of mucin coat thickness. From these results it is speculated that the low implantation rate of in vitro cultured rabbit blastocysts transferred to oviduct of recipient was caused by high degeneration of the embryo after transfer and inappropriate deposition of mucin coat.

We have investigated the properties of the high-latitude cloud MBM 7 using the 3 mm transitions of CO, CS, HCN, HCO+,C3H2,N2H+, and SiO. The molecular component of MBM 7 shows a very clumpy structure with a size of ≤0.5 pc, elongated along the northwest-southeast direction, perpendicularly to an extended HI component, which could be resulted from shock formation. We have derived physical properties for two molecular cores in the central region. Their sizes are 0.1-0.3 pc and masses 1-2 M⊙ having an average volume density ~2×10 3 cm-3 at the peak of molecular emission. We have tested the stability of the cores using the full version of the virial theorem and found that the cores are stabilized with ambient medium, and they are expected not to be dissipated easily without external perturbations. Therefore MBM 7 does not seem to be a site for new star formation. The molecular abundances in the densest core appear to be much less (by about one order of magnitude) than the 'general' dark cloud values. If the depletions of heavy elements are not significant in the HLCs compared with those in typical dark clouds, our results may suggest different chemical evolutionary stages or different chemical environments of the HLCs compared with dense dark clouds in the Galactic plane.

The performance of grass filter strips (GFS) in abating P04-P concentrations from the forage cropland was tested in an experiment on the 10% slope in Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration (RDA) from October 2007 to September 2009. Forage croplands with rye-corn double cropping system applied with chemical fertilizer and livestock manure (LM) were compared in a natural condition. The plots were hydrologically isolated Main plots consisted of the length of GFS, such as Om, 5m, 10m and 15m. Sub plots consisted of the type of LM, such as chemical fertilizer (CF), composted cattle manure (CCM) and composted swine manure (CSM). Concentrations of P04-P in surface runoff water were reduced as the length of GFS increased. Especially, GFS with 10m and 15m reduced PO4-P concentrations significantly compared to that with Om and 5m (p<0.05). The results from this study suggest that GFS improved the removal and trapping PO4-P from forage croplands.

Seeds and seedlings of Acacia bivenosa D.C. Prod, A. salicina Lindl., A. saligna (Labill.) H. Wendl. and A. tumida F. Muell. ex Benth were germinated and grown in NaCl solutions to investigate the relative salt tolerance among the different species, and t