DNA methylation at CpG sites, which is a epigenetic modification, is associated with gene expression without change of DNA sequences. During early mouse embryogenesis, dynamic changes of DNA methylation occur. In this study, DNA methylation patterns of porcine embryos produced in vivo and in vitro were examined at various developmental stages by the immunocytochemical staining method. Interestingly, active demethylation was not observed on the paternal pronucleus of porcine zygotes. However, differences were detected in the passive demethylation process between in vivo and in vitro embryos. There was no change in the DNA methylation state until the blastocyst stage of in vivo embryos, whereas partial demethylation was observed in several blastomeres from a 4 cell stage to a morula stage of in vitro embryos. The whole genome of inner cell mass (ICM) and trophectoderm (TE) cells in porcine blastocysts were evenly methylated without de novo methylation. Our findings demonstrate that genome-wide demethylation does not occur in pig embryos during preimplantation development unlike murine and bovine embryos. It indicates that the machinery regulating epigenetic reprogramming may be different between species.

We introduce and describe performance of the 6-meter telescope of Seoul Radio Astronomy Observatory (SRAO). All the softwares and instruments except the antenna structure and its driving system are developed for ourselves. The SIS mixer type receiver resulted in the receiver noise temperature less than 50 K (DSB) over the whole 3-mm radio window. An autocorrelation spectrometer, developed first in Korea, provides maximum 50 MHz band width over 1024 channels. Antenna surface is measured and adjusted using template method and radio holography which resulted in a superb surface accuracy bet-ter than 30μm. Accordingly, the aperture and beam efficiences amount to 70% and 75%, respectively, largely independent of frequency in the 85 - 115 GHz range. It is also found that telescope pointing errors are less than 10" in both azimuth and elevation and that antenna gain is almost constant against elevation greater than 20°, without adjusting sub-reflector position. The SRAO 6-meter telescope is now fully operational and all these characteristics verify that observations are carried out with high precision and fidelity.

페레니얼 라이그라스에서 질소의 공급형태 ( or ) 및 수준 (0.2, 1.0 and 6.0mM)에 따른 질소동화와 탄수화물 대사산물에 미치는 영향을 알아보기 위해 nitrate, nitrate reductase, sugar 농도와 Fructan 농도를 조사하였다. 공급구에서 잎의 생체량은 약간 증가하다가 같은 수준으로 유지되는 반면 공급구에서는 농도가 증가함에 따라 처음수준에 비해 약 25%에서 30% 증가하였다. 공급구에서 Nitrate 농도는 Ni