The purpose of this study was the analysis of sperm ability in Specific Pathogen Free (SPE) miniature pig for production of bio-organ. The collected semen was diluted with extender and stored at 17℃ for up to 7 days. The semen samples were evaluated at 0, 1, 3, 5, and 7 days of storage for analysis of sperm ability. Sperm ability was evaluated by examining viability, progressive motility, sperm abnormality and intensity of the sperm membrane. Also, the semen was processed according to the convenient freezing method, and frozen-thawed sperm was evaluated by examining viability, capacitation and acrosome reaction using chlortetracycline (CTC) staining. Motility of spermatozoa of SPF miniature pig was significantly (P<0.05) lower on 3 days or later compared to the Duroc, Yorkshire and Landrace in domestic boar. The percentage of abnormal spermatozoa of Landrace were significantly (P<0.05) higher than in SPF miniature pig, Duroc and Yorkshire that had a similar percentage on 5 or 7 days of sperm storage. The percentage of spermatozoa with coiled tail decreased during the storage period but there were no significant difference. On the other hand, viability of frozen-thawed spermatozoa had a significantly (P<0.05) lower in SPF miniature pig than in other domestic boars. CTC patterns had no significant difference, but SPF miniature pig had higher percentage of capacitated spermatozoa and lower percentage of acrosome-reacted it than domestic boars. Therefore, this study suggest that it is necessary to develop the suitable extender and freezing methods methods for the high viable rate and fertilizing ability in vitro.

이탈리안 라이그라스 (Lolium multinorum Lam.)와 톨페스큐 (Festuca arundinacea Schreb.)의 속간 교잡종 3계통에 대한 약 배양을 실시하여 약 유래의 식물체를 생산하였다. MS 기본배지에 sucrose 30 g/, NAA 2 mg/, kinetin 1 mg/을 첨가한 캘러스 유도배지에서 배양 20일만에 약 유래의 캘러스를 유도하였다. 캘러스 유도율은 평균 11.6%였으며, 캘러스의 크기는 평균 9.1 mg/callu

축산기술연구소 사료작물 육종연구실에서는 1984년부터 이탈리안 라이그라스의 기호성과 페스큐류의 높은 환경적응성을 결합하기 위해 Lolium X Festuca 잡종을 생산하였고, 1991년부터 이탈리안 라이그라스 생태형을 수집하여 특성을 검정하고 계통화하여 왔다. 1997년부터 이들 교잡계통과 생태형 중에서 임성이 있고 내한성이 강하며 출수기가 비슷한 영양계통으로 교배조합을 작성하여 Polycross 삼각배치법으로 합성종 내한 6, 7, 8, 9호를 육성