A 7-year-old, intact, female Siberian husky was presented to the Veterinary Medical Center of Chungbuk National University because of vomiting and diarrhea after a fight with a cohabiting dog. Physical examination, radiography, abdominal ultrasonography, and laboratory examination were performed. The dog was diagnosed with pyometra and 10% dehydration. On electrolyte, Na and Cl ion concentration were lower than normal values and Ca ion concentration was slightly lower than normal values. On blood chemistry, blood urea nitrogen and alkaline phosphatase value were extremely higher than normal values. Neutrophil was observed as hypersegmentation. On ultrasonography, enlarged uterine body was observed. Uterus was enlarged and filled with echogenic fluid that is seemed to be pus. Uterine horn was rubbing the bladder. In bacterial culture, the colony morphology was smooth, mucoid, and hemolytic. Also, on molecular diagnosis test, both samples from uterine and abdominal fluid were confirmed to be E. coli. This case describes that uterine pus was leakage to abdominal cavity through oviduct, and pus leakage from uterus may be cause septic peritonitis and death. Also, this case must be considered that physical event such as fight against cohabiting dog, strenuous exercise and kiss-off can cause uterine rupture or pus leakage through oviduct from uterus in dog with pyometra.

This study aimed to examine the effect of a mild elevation in serum cholesterol level in a porcine coronary overstretch restenosis model using a balloon angioplasty catheter or drug-eluting coronary stent. Pigs were divided into two groups and were fed a commercial normal diet (CND, n = 4) or a high-fat diet (HFD, n = 4) for 5 weeks. Coronary overstretch injury by balloon angioplasty or stent implantation was induced in the left anterior descending and left circumflex artery after 1 week of feeding. Histopathological analysis was performed at 4 weeks after coronary injury. During the experiment, the total cholesterol level in the HFD group increased by approximately 44.9% (from 65.9 ± 3.21 mg/dL at baseline to 95.5 ± 9.94 mg/dL at 5 weeks). The lumen area in the CND group was reduced in comparison with that in the HFD group after balloon angioplasty. After stent implantation, the injury score showed no significant difference. There were significant differences in the neointimal area (2.7 ± 0.33 mm2 in the CND group vs. 3.3 ± 0.34 mm2 in the HFD group, p<0.05), lumen area (2.6 ± 0.54 mm2 in the CND group vs. 2.0 ± 0.33 mm2 in the HFD group, p<0.05), and percent area stenosis (52.0 ± 7.96% in the CND group vs. 62.4 ± 5.15% in the HFD group, p<0.05). Body weight change was not different between the two groups. Increased serum cholesterol level activated vascular smooth muscle cell proliferation in the porcine coronary overstretch model.

In this study, steric height variability in the East Asian Seas (EAS) has been analyzed by using ocean reanalysis intercomparison project (ORA-IP) data. Results show that there are significant correlations between ocean reanalysis and satellite data except the phase of annual cycle and interannual signals of the Yellow Sea. Reanalysis ensemble derived from 15-different assimilation systems depicts higher correlation (0.706) than objective analysis ensemble (0.296) in the EAS. This correlation coefficient is also much higher than that of the global ocean (0.441). For the longterm variability of the thermosteric sea level during 1993-2010, a significant warming trend is found in the East/Japan Sea, while cooling trend is shown around the Kuroshio extension area. For the halosteric sea level, a dominant freshening trend is found in the EAS. However, below 300 m depth around this area, the signal-to-noise ratio of the linear trend is generally less than one, which is related to the low density of observation data.

Although the use of Grammatical Metaphor (GM) has been regarded as one of the representative features of academic writing in the framework of Systemic Functional Linguistics (SFL), not many studies have explored its role in second or foreign language writers’ writing quality. This study aimed to discover the potential relationship between GM development and argumentative writing quality of advanced Korean EFL learners, while controlling for their overall language proficiency and reading comprehension ability. The findings from this study unveiled significant contribution of the use of both premature and mature GM and also identified transcategorization as the specific GM that has relatively stronger predictability of writing quality. The results from current research yield meaningful pedagogical implications for writing instruction in Korean EFL contexts.

The use of non-therapeutic antibiotics as animal feed additives has raised public health concerns due to the increasing resistance of pathogens to antibiotics. It is therefore required to develop safe and effective alternative feed additives to replace non-therapeutic antibiotics. The aim of this study was to assess the effects of the multiherbal compound, KIOM-C, on growth performance and immune response of growing-to-finishing pigs under farm conditions. The experimental trials were performed in a Korean commercial swine growing-to-finishing complex, and a total of 70-day-old 160 pigs were selected. Eighty pigs were treated with KIOM-C at the level of 2 kg/tonne until slaughter age (KT group), while another 80 pigs were not treated with KIOM-C (NT group). All animals were vaccinated against foot-and-mouth disease (FMD) at 60 and 110 days of age. During the trial period, average daily weight gain (ADWG), average daily feed intake (ADFI), feed conversion ratio (FCR), survival rates, and average slaughter ages were measured. The serum concentrations of tumor necrosis factor-a (TNF-α), interferon-γ (IFN-γ), and IgA were also evaluated. In order to evaluate specific humoral immune responses, the foot-and-mouth disease virus (FMDV) serotype O-specific antibody was measured. The ADWG, ADFI, and FCR of the KT group were significantly greater than those of the NT group (p<0.05). Serum concentrations of IgA in the KT group was statistically higher than the NT group. The antibody levels of the KT group against FMDV serotype O was higher than the NT group, and 86.67% of the KT group tested positive for anti-FMDV antibodies. Overall, these findings suggest that KIOM-C improves growth performance and immune response of pigs under growing-to-finishing farm conditions, and implies that the herbal compound may be used as a suitable alternative feed additive.

Pigs are considered as optimal donor animal for the successful xenotransplantation. To increase the possibility of clinical application, genetic modification to increase compatibility with human is an important and essential process. Genetic modification technique has been developed and improved to produce genetically modified pigs rapidly. CRISPR/Cas9 system is widely used in various fields including the production of transgenic animals and also can be enable multiple gene modifications. In this study, we developed new gene targeting vector and enrichment system for the rapid and efficient selection of genetically modified cells. We conducted co-transfection with two targeting vectors for simultaneous inactivation of two genes and enrichment of the genetically modified cells using MACS. After this efficient enrichment, genotypic analysis of each colony showed that colonies which have genetic modifications on both genes were confirmed with high efficiency. Somatic cell nuclear transfer was conducted with established donor cells and genetically modified pigs were successfully produced. Genotypic and phenotypic analysis of generated pigs showed identical genotypes with donor cells and no surface expression of α-Gal and HD antigens. Furthermore, functional analysis using pooled human serum revealed dramatically reduction of human natural antibody (IgG and IgM) binding level and natural antibody-mediated cytotoxicity. In conclusion, the constructed vector and enrichment system using MACS used in this study is efficient and useful to generate genetically modified donor cells with multiple genetic alterations and lead to an efficient production of genetically modified pigs.