Using the Keck 10 m telescope data with the HIRES spectrograph, we analyzed the evolution of Lymanα forest clouds at z > 2 down to the HI column density 10 12.8cm -2. The number density per unit column density does not change with redshifts at lower HI cloumn density (NHI < 10 14cm-2), while the forest clouds at higher column density disappear rapidly. The cutoff b value, the thermal temperature indicator, increases as redshift decreases. The correlation strength seems to be stronger as redshift decreases.
In conclusion, tremendous potential exists for the application of animal biotechnology to the beef industry, especially with the utilization of embryo cloning to produce genetically identical animals and genetic engineering to modify animal genomes to improve and /or create new phenotypes for many economically important traits. Research involving embryo cloning and genetic engineering of animals has been continuous now for over a decade, however inefficiencies in techniques have prevented large scale application. large numbers of identical cattle will some day be produced and producers will be utilizing transgenic cattle in their beef production programs.
본 논문은 수식화의 특이성 때문애 구조 최적화 문제에 거의 사용되지 않고 있는 선형 goal programrrung을
대규모 비선형 구조 최적화에 용용하는 방법올 제시한다. 이 방법은 다기준 최적화의 도구로 사용
되는데 그 까닭은 goal programming 이 목적합수와 제한조건둥을 정의하는데 있어서 발생하는 난점 들올
제거해 주기 때문이다.
이 방병은 비선형 goal 최적화 문제톨의 해톨 얻기 위해서 유한요소해석, 선형 goal programming 기볍 ‘
그리고 계속적인 선형화 기법을 이용한다. 즉, 대규모 비선형 구조 최적화 문제를 비선형 goal programming
형태로 전환시키는 일반적인 수식화 방법을 제시하고, 얻어진 비선형 goal 최적화 문제 를 풀기 위한
계속적인 선형화 방법에 대해서도 논의한다. 얼계도구로서 이 방법의 유효성올 논증하기 위하여 10‘ 25
및 200트러스의 사례를 가지고 용력채한조건들의 최소무게 구조 최적화 문제에 대한 해를 모색하며 이 쓸
다른 연구결과와 비교검토한다.
Bacillus thuringiensis 변종(變種)들로부터 Extrachromosomal DNA를 추출분리(抽出分離)코저 종래(從來)의 방법(方法)을 보완(補完)하여 적용(適用)한바 분자량(分子量)의 크기가 1 Megadalton에서 135 Megadalton에 이르는 plasmid들을 분리(分離)함에 보다 효과적(效果的)이었고 또 이 plasmid들을 이용(利用), 제한효소(制限酵素)에 의(依)한 유전자배열작성(遺傳子配列作成) 및 gene Cloning을 하는데 비교적(比較的) 안정(安定)된 많은 양(量)의 세포용해물(細胞溶解物)을 얻을 수 있었다. 파리목과 나비목에 각기(各其) 독성(毒性)이 다른 Bacillus thuringiensis 6개(個) 변종(變種)으로부터 plasmid들을 분리(分離)한 결과(結果) 분자량(分子量)이 큰 50 Megadalton 이상(以上)의 plasmid들이 공시(供試) 된 모든 변종(變種)으로부터 추출(抽出)되었으며 이들 plasmid의 수(數)를 보면 israelensis로부터 8개(個) kurstaki로부터 10개(個) 로부터 13개(個) dendrolimus로부터 2개(個), finitimus로부터 1개(個) 그리고 yunnanensis로부터 6개(個)가 각각(各各) 검출(檢出)되었다. 공시(供試)된 변종중(變種中) 4개(個)의 변종(變種)으로부터는 2 Megadalton 이하(以下)의 적은 plasmid들도 추출(抽出)되었다.
The aim of this article is to investigate the relationship between insurance and economic growth at aggregate and disaggregate level for the period 1982-2018. Very few studies have been carried out in this field, with contradictory results and using an aggregate data while, according to different authors, an aggregate data might provide spurious results. The author used Ordinary Least Squares Regressions (OLS) and Granger Causality tests to explore the strength and direction of the relationship between insurance and economic growth at an aggregate level. To check the relationship at disaggregate level life insurance, marine insurance, and property insurance are regressed on trade openness and investment, respectively. Non-life insurance at an aggregate level plays a positive and significant role in promoting economic growth, but life insurance has an insignificant impact on the Pakistan economy. On the other hand, non-life insurances at a disaggregated level such as marine insurance negatively affect a vital part of economic growth, i.e., trade. At the same time, property insurance has a significant and positive role in boosting investment. Life, marine, and property insurance Granger cause economic growth, trade, and investment in a single direction. Nevertheless, is a bi-directional relationship between economic growth and non-life insurance.
The article compares economic and environmental performance of Taipei and Almaty from the point of view of "green" economy, which is able to act as a key tool to ensure sustainable development of the region. As the comparison of the parameters of ecological and economic development of the Taipei and Almaty cities shows, they are similar in population size, but demonstrate completely different trends of sustainable development. Economic performance of the Taipei city is achieved with a decrease in the consumption of natural resources and the production of pollution, while the situation in Almaty is the opposite. Almaty maintains a high level of air pollution due to the use of coal in electricity production, as well as increased traffic and density of construction, including the southern part of the city, which is a zone for the transit of mountain air flows. The article discusses the activities jointly conducted by the Government of Taiwan and non-governmental organizations on environmental issues, as well as environmental NGOs, which resulted in significant improvements in the environmental field. Measures to stimulate the development and implementation of environmental innovations applied in the field of sustainable development in the city of Taipei can be adapted for the city of Almaty, where the environmental situation deteriorates year by year.
Optical interferometry and polarimetry have separately provided new insights into stellar astronomy, especially in thefields of fundamental parameters and atmospheric models. We present: scientific justifications for “full-Stokes” opticalinterferometric polarimetry (OIP); updated instrument requirements; preliminary beam combiner designs; polarimeterdesign; end-to-end OIP data reduction; and realistic reimaged full-Stokes models of Be stars with a suitable number oftelescopes plus noise sources. All of this work represents preliminary research to construct an OIP beam combiner.
Controllable transgenic expression systems in transgenic animal model are valuable to the development of therapeutic approaches in human medical fields. The aim of this study was to 1) produce a transgenic cloned dog using inducible tetracycline vector system, and 2) investigate whether the transgenic cloned dog could be induced the transgene expression using doxycycline (Doxy). Canine fetal fibroblasts were infected with retroviral vectors designed to express the enhanced green fluorescent protein (eGFP) gene under the control of tetracycline-inducible promoter. For somatic cell nuclear transfer (SCNT), nucleus of an in vivo matured oocyte was removed and an eGFP expressed cell cultured with 1 ㎍/㎖ of Doxy was injected. After electrical fusion and chemical activation, the reconstructed embryos were transferred to a recipient and pregnancy diagnosis was performed by ultrasonography. Experiment I evaluated the mean fluorescence intensity (MFI) of infected cells while the cells were cultured in the presence of 1 ㎍/㎖ of Doxy for 5 days, and then in the absence of Doxy for 7 days using fluorescence-activated cell sorter. Experiment II was designed to produce an eGFP controllable transgenic cloned dog via SCNT. For verification of transgenic dog, experiment III was performed Southern Blot analysis and observation in vivo regulation of eGFP expression in the cloned dog treated with 100 ㎎/㎏ of Doxy every 2 days for 2 weeks under ultraviolet light. In experiment IV, western blot was used to detect eGFP increase and decrease in skin tissues of transgenic dog under the presence or absence of Doxy. In the results of experiment I, the MFI for infected cells was rapidly increased to approximately 42.3 times after 3 day-treatment compared to pre-treatment and quickly decreased 3 days after ceasing the treatment. In experiment II, a total of 203 embryos were transferred to nine recipients and three pregnant delivered three pups (Tet-on eGFP 0, Tet-on eGFP 1, and Tet-on eGFP 2) by C-sec and Tet-on eGFP 2 among them is still alive. All cloned pups were genetically identical to the donor cell. Tet-on eGFP 2 showed an apparent in vivo eGFP expression on her body after Doxy administration in experiment III. The result of Sothern blotting showed that the transgene insertion was detected from the three cloned dogs and all organs of Tet-on eGFP 1. Experiment IV indicated that a robust eGFP expression in skin tissue of Tet-on eGFP 2 rapidly increased after Doxy treatment and gradually decreased to basal level on 9 weeks after ceasing the treatment. In conclusion, we report here for the first time an inducible transgenic system in canine species and it can stably induce the transgene expression at intended time. This study has demonstrated the capacity to generate transgenic model dog which could regulate the transgene and it would contribute to human medical research fields.
The cloning of canids was succeeded in 2005, several years after the birth of Dolly the sheep and also after the cloning of numerous other laboratory and farm animal species. The delay of successful somatic cell nuclear transfer (SCNT)was due to the unique reproductive characteristics of the female dogin comparison to other domestic mammals, such as ovulation of immature canine oocyte and a requirement of 25 days for the completion of meiosis within the oviduct (Holst & Phemister, 1971). When the technology for the recovery of in vivo matured oocyte was established, the application of cloning also became possible and cloned dog offspring were obtained. This report summarizes the progress of technical procedures that are required for cloning canids and the application of this technique. The first cloned dog, Snuppy, was achieved using an in vivo-matured oocyte which was enucleated and transferred with an adult skin cell of male Afghan hound. After establishment of a criterion of well-matured oocyte for the improvement of SCNT efficiency, we obtained three cloned female Afghan hound and a toy poodle cloned from 14 year-old aged Poodle using SCNT through this factor. To date, cloned dogs appeared to be normal and those that have reached puberty have been confirmed to be fertile. Through application of canine SCNT technique, first, we demonstrated that SNCT is useful for conserving the breed of endangered animal from extinction through cloning of endangered gray wolves using inter-species SCNT and keeping the pure pedigree through the cloning of Sapsaree, a Korean natural monument. Secondly, we showed possibility of human disease model cloned dog and transgenic cloned dog production through cloning of red fluorescent protein expressing dog. Finally, SCNT can be used for the propagation of valuable genotypes for making elite seed stock and pet dog. In summary, dog cloning is a reproducible technique that offers the opportunity to preserve valuable genetics and a potential step towards the production of gene targeted transgenic cloned dogs for the study of human diseases.