Nitric oxide(NO) is synthesized via the oxidation of L-arginine by a family of nitric oxide synthases(NOS), which are either constitutive(cNOS) or inducible(iNOS). The induction of iNOS in tissues can lead to the sustained production of high concentrations of NO which may exert pro-inflammatory effects including vasodilation, edema, cyototoxicity, and its activity can be mediated by various pro-inflammatory cytokine, including interferonγ(INF- γ), tumor necrosis factor, IL-1 and IL-6. The enzyme, iNOS, became a new target for pharmacologcal research with the aim to find new substances for the treatment of chronic inflammatory disorders. Murine macrophages produce large amounts of NO when activated with TFN-γ plus LPS. Murine macrophage-like cell line, RAW 264.7, is a suitable cell model to perform in vitro studies regarding the iNOS system. Artemisin feddei Lev. et Vnt.(Compositae) is a perennial herb growing in Korea. The aerial parts have been used in foik medicine as antiinflammatory, antipyretic, choleretic and diuretic agent. Sesquiterpenelactones were isolated from this plant. In the course of screening for NO inhibitory activity from medicnial plants, the aqueous extract of this plant was found to have a significant activity.

Anal Therapy is another way of taking medicine. It is a traditional pathway but not available in common situation. Nevertheless, It has many benifect and usefulness, it has not treated so much. As there are many kinds of Anal therapy, this study use retention type. Sochungyongtang (SCYT) has been known to be the most effective prescription largely applied to bronchial asthma, whooping cough, pneumonia, pleurisy, nephritis and arthritis and so forth. In this study, the author investigated the effect of an aqueous extract of SCYT by Anal therapy (Retention Enema) in immediate type allergic reaction. The following results were obtained 1. Retention Enema of SCYT showed a marked inhibition rate in systemic immediate-type allergic reaction with a dose of 1000 mg/kg 1 hr before intraperitoneal injection of compound 48/80. 2. SCYT significantly reduced serum histamine contents induced by compound 48/80. SCYT (1000 mg/kg) also inhibited to 64.11±19.86% (P<0.01) local immediate-type allergic reaction activated by anti-dinitrophenyl (DNP) IgE. 3. In addition, SCYT dose-dependently inhibited the histamine release from the peritoneal mast cells by compound 48/80 or anti-DNP IgE. These results provide evidence that anal therapy (Retention enema) of SCYT may be beneficial in the treatment of systemic and local immediate-type allergic reaction.