The Hangang River, serving as the primary water supply source for the capital region of South Korea, plays a vital role in sustaining regional ecosystems and ensuring water quality. Phytoplankton are integral to the maintenance of aquatic ecosystems and function as bioindicators of water contamination; however, the species-level composition and environmental interactions of phytoplankton in the Hangang River remain inadequately characterized. This study investigated the phytoplankton communities within the Hangang River system across eight sampling stations over eight sampling events from May 2021 to March 2022. A total of 71 species were identified, yielding a density of 3,560 cells mL-1, with Bacillariophyceae (36 species, 2,809 cells mL-1), Chlorophyceae (24 species, 212 cells mL-1), and Cyanophyceae (5 species, 394 cells mL-1) identified as the dominant groups. The most abundant species was Aulacoseira granulata (1,616 cells mL-1, 45.4%), followed by Stephanodiscus hantzschii (420 cells mL-1, 11.8%) and Phormidium tenue (383 cells mL-1, 10.8%). Spatial similarity analysis of the phytoplankton communities revealed three distinct groups: Group A (stations 1-4, upstream), Group B (stations 5-7, midstream), and Group C (station 8, downstream). Seasonal similarity analysis further categorized the communities into three groups: Group A (May, July, August, and October 2021), Group B (November and December 2021), and Group C (February and March 2022). These findings contribute to a deeper understanding of the spatial and seasonal dynamics of phytoplankton in the Hangang River system.