To investigate the differential expression of genes by 635nm LEDs irradiation in arachidonic acid-treated human gingival fibroblasts, cDNA microarray was carried out. Human gingival fibroblasts were primary cultured and arachidonic acid was treated to induce inflammation. 635nm of wave length was used for LEDs irradiation. The experimental group was categorized into four group ; control, only LEDs irradiation group, only arachidonic acid-treated group and arachidonic acid-treated with LEDs irradiation group. The expression of 8,078 genes were increased and the expression of 7,103 genes were decreased in only LEDs irradiation group. For arachidonic acid-treated with LEDs irradiation group, the expression of 6,815 genes were increased, while the expression of 8,031 genes were decreased comparing with only arachidonic acid-treated group. IL-13alpha2 receptor was the most expressed gene in LEDs irradiation group comparing with control, followed by MMP3. Genes which the most down regulated was BIRC3 in LEDs irradiation group. PLAB genes was the most up-regulated in arachidonic acid treated with LEDs irradation group, followed by ranked RARRES1. Considering the classification by cell function, genes associated with signal transduction were the most affected by LEDs irradiation, followed by the genes associated with nucleoside, nucleotide and nucleic acid metabolism. In arachidonic acid treated with LEDs irradiation, genes associated with signal transduction and protein metabolism were affected. Taken together, LEDs irradiation could affect various biological process and could identify many genes related to LEDs irradiation, which could be used for clinical application.

Department of Oral Pathology, Dental Science Research Institute, College of Den디'suy， Chonnam National University It is well known that the expansion of radicular and dentigerous cyst is related to the change of osmotic pressure, while the prolifera디on ofthe 비피19 epithelium in odontogenic keratocyst. When the dentigerous cyst and odontogenic keratocyst has secondary infection, they present the loss of unique sπuctures and epithelial hyperplasia. There is a question whether inflammatory reaction affects cystic formation, expansion and their treatment. Present study is to evaluate the relationship between inflammation and epithelial hyperplasia using immunohistochemial study. Followings are result; 1. 까1e age of patients ranged from 10 to 73 years (mean age, 39 years) in radicular 이5t， 10 to 71 years (mean age, 35 years) in dentigerous cyst and 10 to 54 years (mean age, 23.4 years) in odontogenic keratocyst. The sex distribution of patient distributed 32 cases for male and 16 cases for female in radicular cyst, 13 and 10 in dentigerous cyst and equally 5 and 5 in odontogenic keratocyst. 2. Inflammatory cyst in the present study distributed 44/48 cases (9 1.7%) for radicular cyst, 15/ 23 cases for dentigerous cyst and 1/ 10 case for odontogenic keratocyst. 3. Evaluation of inflammatory reaction and antigen expression, ki-67 , cox-2 and glut-1 expression increase in the inflammatory radicular and dentigerous cyst. 4. In odontoge띠c keratocyst, expression of antigens increase regardless of inflammation. Above results showed that inflammation stimulate the proliferation of lining epithelium leading to cystic expansion.