본 연구는 능소화를 대상으로 화분형태 및 RAW264.7 대식세포에서의 세포생존에 미치는 영향을 조사하여 유해성 여부를 파악하고자 한다. 주사전자현미경을 이용한 화분 형태를 확인해 본 결과, 화분립 형태는 장구형이고 발아구의 형태는 3공구형이며 화분 표벽 (extine)은 망상의 형태이고 외부로 돌출된 돌기는 관찰되지 않았다. 능소화 70% MeOH 부위별 (꽃, 잎, 줄기) 추출물 및 화밀을 24, 48시간 동안 농도별 (10, 20, 50, 100 μg mL-1)로 RAW264.7 대식세포에 처리한 후 MTT assay로 측정하였다. 그 결과, 능소화 추출물 및 화밀을 농도별로 24시간 처리하였을 때 모든 농도에서 99.0% 이상의 세포생존율을 보여 세포독성은 나타나지 않았다. 48시간 처리하였을 때 꽃, 잎, 줄기 추출물은 50 μg mL-1농도 이하에서 세포독성은 나타나지 않았으나, 화밀의 경우 저농도인 10 μg mL-1부터 농도의존적으로 세포독성이 높아지는 것을 확인하였다.

β-cyclodextrin has an ability to protect compounds from oxidative reaction by collecting them within its ring-like structure. So, In harsh condition (40oC), marker compound, quercetin, was dramatically reduced in Hovenia dulcis fruit extract containing dextrin at 4 and 8 week compared to 0 week, but not that containing β-cyclodextrin. To evaluate the effects of dextrin and β-cyclodextrin on protective effect of H.dulcis fruit extract against alcohol- induced liver damage, The mice were orally injected alcohol, H. dulcis fruit extract/dextrin (HD) and H. dulcis fruit extract/β-cyclodextrin (HCD), respectively, for 7 days. The mice orally administrated with alcohol significantly enhanced the serum concentration of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the activity of lactate dehydrogenase (LDH) in serum compared to the control group. HD and HCD significantly decreased the levels of serum ALT and AST and serum LDH activities compared to alcohol group. And also alcohol group significantly increased the level of total cholesterol compared to the control group, but HD and HCD significantly reduced it compared to the alcohol group. However, the levels of TG in blood were not significantly changed in all groups. The activities of alcohol dehydrogenase (ADH) were significantly increased in HD and HCD group although those of aldehyde dehydrogenase showed an increasing tendency. This data suggested that HD and HCD were able to induce alcohol degradation in the liver tissues. All together, the results showed that HCD demonstrated their ability to protect liver from alcohol-induced damage on equal terms with HD.

TThis study investigated the influence of AGH-Ringer (chelated organic minerals liquid fertilizer) on the growth, yield, and fruit quality of ‘Jukhyang’ strawberry in a hydroponic system. The results revealed that AGH-Ringer group led to significantly higher plant length than that of the control group, and that leaf length and leaf width increased with increasing leaf area. In addition, the fresh and dry weights of leaves and SPAD unit, which measures leaf color also showed a significant increase with AGH-Ringer. However, the length and dry weight of roots did not show significant differences when compared to the control group. In the fruit quality analysis, AGH-Ringer group did not show any difference in fruit length compared to the control group, but it increased both the fruit weight, which, along with the simultaneous increase in fruit width, increased the yield per strawberry. Furthermore, AGH-Ringer group strengthened the red skin color of the fruit, and resulted in significantly greater hardness than that of the control group, but did not have any effect on the soluble solid content and acidity. As shown in the results, the AGH-Ringer group resulted in lesser mineral content and lower to medium EC (electrical conductivity) in comparison to the inorganic mineral hydroponic fertilizer, which was used as a control. However, it increased the leaf growth, fruit weight, and yield, and improved the fruit quality by increasing the soluble solid content in the hydroponic system of ‘Jukhyang’ strawberry. Therefore, based on this study, AGH-Ringer is thought to be a more effective hydroponic fertilizer than the inorganic mineral hydroponic fertilizer. Moreover, the field experiment demonstrated that AGH-Ringer is a useful hydroponic fertilizer convenient for application in farms. This study also proved that AGH-Ringer is a fertilizer that can promote plant nutrition by controlling the salinity of the soil and facilitating the absorption of necessary minerals in future soil cultivation, thereby providing basic data for organic farming.