We investigated biomass, diatom species and fucoxanthin contents as cell growth, fatty acid and amino acid contents as nutritional composition of diatoms attached on plate to confirm effects of light emitting diodes (LEDs) due to block off natural light. In the single LED irradiation, biomass showed significantly higher to 30.0±6.48mg/m2 in white LED than that of others (P<0.05). The dominate diatom species was Navicula cancellata. Their lipid contents showed significantly higher to 112.9±19.23ug/mg dry matter (DM) in control than that of others LEDs. But eicosapetaenoic acid (EPA) contents showed significantly higher to 3.3±0.62ug/mg DM than others, but not significantly differed with natural control light treatment (P<0.05). And total protein contents are higher in control and blue LED light than that of others, but essential amino acid contents showed significantly higher to 3.2±4.8% in control (P<0.05). In mixing light with natural and LED light, biomass showed 2.6±0.22mg/m2 in blue LED (P<0.05). Fatty acids contents were not significantly differed with all treatments. Amino acid contents showed to 11.0±0.33ug/mg DM in white LED (P<0.05), but not significantly differed with others LED lights (P>0.05). Therefore, we could suggest that irradiation of blue LED in natural light very benefit to diatom culture for larvae of sea cucumber and abalone and do on.

The marine microalgae Chaetoceros calcitrans was cultured under a fluorescent lamp (CON) and light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; green, LG; white, LW); changes in growth, fucoxanthin, chlorophyll-a, amino acid and fatty acid profiles were investigated. LR-exposed cultures exhibited the highest specific growth rate (SGR) (0.34), whereas LG-exposed cultures showed the lowest SGR (0.26). After cultivation for 10 days, the maximum dry cell weight (g/L) of LR-exposed cultures was significantly higher than that of those exposed to other light conditions (LR≥ CON>LB≥LW≥LG). Eicosapentaenoic acid (EPA) levels were significantly higher in CON-exposed cultures compared to those exposed to LW (P<0.05), with no marked difference compared to those exposed to LB, LR and LG (P>0.05). The fucoxanthin content was highest in LB-exposed cultures (6.3µg/mL), whereas LW showed the lowest (3.6µg/mL; P<0.05). Chlorophyll-a content was highest in cultures exposed to LB compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.

Information about the density of fish eggs is important to understand the vertical distribution of eggs and survival in early stage, in particular change in egg density is one of major issue in fisheries. This paper describes a practical application of an experimental system for measurement of the fish egg density under laboratory conditions. The device can control the temperature range in each water column, and make different densities at each layer. The density of olive flounder (Paralichthys olivaceus) eggs have ranged from 1,018.49 to 1,020.93 kg/m3 and were aggregated around the density of 1,020 kg/m3. The results show that the device is applicable for measuring the fish egg density under laboratory conditions.