Pure Bombus ignitus venom samples were submitted to two-dimensional gel electrophoresis. A total of 64 excised spots were analyzed by mass spectrometry. Three main proteins resulted in the identification have not been described in other bee venoms before. Dose-dependence against human carcinoma (Hep3B, BT-20, A549 and AGS) were observed from 1 ng/㎖ to 100 ng/㎖. Expecially, the treatment of 100 ng/㎖ B. ignitus venoms showed the highest cytotoxicity with 55% against hepatocellular carcinoma (Hep3B). The B. ignitus venoms showed strong antimicrobial activities against Enterococcus faecium and Shigella sonnei, and practically antimicrobial activity against the other microorganisms tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of E. faecium and S. sonnei, were 0.256 ug/㎖, respectively.
경상북도내의 금속광산, 공장 및 공업지역의 토양의 오염도를 조사하고 중금속 오염토양 중에 분포하는 미생물 flora의 정량적 평가를 위하여 청정지역인 독도토양과 비교한 결과, 일반세균의 경우 10~100배 낮은 분포율을 나타내었으며, 6가크롬 오염토양 중 곰팡이는 거의 검출되지 많았다. 방선균은 수은 오염토양의 경우 6.7X105~7.5X107 cfu g-1, 6가크롬 오염토양 내 방선균 수는 4.8X104~5.0
경상북도내의 금속광산, 공장 및 공업, 일반폐기물, 공단주거지역 및 특수 용수사용지역 등 360곳의 토양을 2001년 3월부터 10월가지 채취하여 토양 오염도를 조사하였다. 토양 오염도는 중금속인 카드뮴, 구리, 비소, 수은, 납, 6가 크롬 및 시안의 7개 항목을 대상으로 하였으며, 그 중 각 항목에서 가장 오염도가 심한 5개 지점을 대상으로 토양내 곤충병원성 선충의 분포를 조사하였다. 토양내 선충은 누에를 이용하여 곤충병원성, 사물기생성 선충을 대상
Forests are valuable natural resources for people living around the mountains. In particular, the comfortable feeling or healing is one of the most important benefits obtained from forests. This healing can be possible by many aspects of forests, including the landscape, natural sounds, anions, and pleasant aromas. We focused on the volatile organics from forest causing pleasant aromas, phytoncides. Twenty phytoncides were monitored from February to September in a national tree garden (BaekDoDaeGan SooMokWon). Five sites were monitored two times per month and 20 phytoncides were detected. Borneol showed the highest annual average concentration and the order of concentration was borneol > mycene > sabinene > limonene > α-pinene. The average phytoncide concentration was relatively high in spring and summer season when the trees were physiologically active. Daily monitoring showed that the afternoon hours had higher concentrations of phytoncides than the morning hours, which may be due to the stabilized atmospheric conditions at the sites. Among the five sites, coniferous forests gave higher phytoncide emissions than broadleaf tree forests. The current study showed that forests produce several phytoncides that cause a healing effect and a forest bath may be beneficial to the health of visitors to forests.