The Automobile HVAC system is a habitat for odor-associated fungal communities. We investigated the odorassociated fungal community in an automobile HVAC system using a high-throughput DNA sequencing method. The fungal community structure was evaluated via metagenome analysis. At the phylum level, Ascomycota and Basidiomycota were detected, accounting for 43.41% and 56.49% of the fungal community in the HVAC system, respectively. Columnosphaeria (8.31%), Didymella (5.60%), Davidiella (5.50%), Microxyphium (4.24%), unclassified Pleosporales (2.90%), and Cladosporium (2.79%) were abundant at phylum of Ascomycota and Christiansenia (36.72%), Rhodotorula (10.48%), and Sporidiobolus (2.34%) were abundant at phylum of Basidiomycota. A total of 22 genera of fungi were isolated and identified from the evaporators of the HVAC systems which support fungal growth and biofilm formation. Among them, Cladosporium, Penicillium, Aspergillus and Alternaria are the most representative odor-associated fungi in HVAC systems. They were reported to form biofilm on the surface of HVAC systems with other bacteria by hypha. In addition, they produce various mVOCs such as 3-methyl-1-butanol, acetic acid, butanoic acid, and methyl isobutyl ketone. Our findings may be useful for extending the understanding of odor-associated fungal communities in automobile HVAC systems.

This study investigated the odor-associated bacterial community in automobile HVAC systems. Through a metagenome analysis, it was found that; Massilia (42.426%), Sphingomonas (28.200%), (10.780%), and Methylobacterium (5.756%) were abundant in the HVAC systems. Massilia can cause the biodegradation of polycyclic aromatic hydrocarbons (PAHs) producing odor in automobiles. Sphingomonas produces volatile halogenated compounds or degrades organic pollutants. Rhodococcus is reported to produce sulfur compounds which give off an odor similar to rotting eggs and cabbages. Methylobacterium is one of the most representative bacteria that causes odor in automobile HVAC systems. The evaporator is considered as the appropriate habitat for microorganisms in automobiles because of its high humidity and organic adsorption. Massilia, Sphingomonas, Rhodococcus, Methylobacterium, Bacillus, Staphylococcus, Arthrobacter, Micrococcus, and Pseudomonas, listed in order from most to least present, were isolated as abundant bacteria in the evaporator of the HVAC systems.