This study was conducted to help manage total floating bacteria and fungi in the indoor air by studying the characteristics of total floating bacteria and fungi according to the indoor CO2 concentration of daycare centers. The sampling and analysis of samples was based on the indoor air quality process test method, and the result analysis was conducted using the SPSS statistical program to perform correlation and regression analysis. Correlation and regression results show that CO2 and total airborne bacteria showed positive relationships, but airborne mold did not show relevance. In addition, in order to identify factors affecting airborne mold, correlation analysis and regression analysis were performed regarding total airborne bacteria, PM10, PM2.5, HCHO, outdoor mold, I/O ratio, indoor temperature/ humidity, area per classroom and volume. The results showed that the factors affecting airborne mold were I/O ratio, outdoor airborne mold, and total airborne bacteria. Research results show that CO2 and total airborne bacteria can be reduced and controlled by natural ventilation, and in the case of airborne mold, mechanical forced ventilation such as hoods will be necessary due to the introduction of outdoor airborne mold. In addition, it is necessary to consider I/O ratio criteria in order to confirm effective indoor mold contamination, taking into account the effect of outdoor mold inflow.

The Automobile HVAC system is a habitat for odor-associated fungal communities. We investigated the odorassociated fungal community in an automobile HVAC system using a high-throughput DNA sequencing method. The fungal community structure was evaluated via metagenome analysis. At the phylum level, Ascomycota and Basidiomycota were detected, accounting for 43.41% and 56.49% of the fungal community in the HVAC system, respectively. Columnosphaeria (8.31%), Didymella (5.60%), Davidiella (5.50%), Microxyphium (4.24%), unclassified Pleosporales (2.90%), and Cladosporium (2.79%) were abundant at phylum of Ascomycota and Christiansenia (36.72%), Rhodotorula (10.48%), and Sporidiobolus (2.34%) were abundant at phylum of Basidiomycota. A total of 22 genera of fungi were isolated and identified from the evaporators of the HVAC systems which support fungal growth and biofilm formation. Among them, Cladosporium, Penicillium, Aspergillus and Alternaria are the most representative odor-associated fungi in HVAC systems. They were reported to form biofilm on the surface of HVAC systems with other bacteria by hypha. In addition, they produce various mVOCs such as 3-methyl-1-butanol, acetic acid, butanoic acid, and methyl isobutyl ketone. Our findings may be useful for extending the understanding of odor-associated fungal communities in automobile HVAC systems.

This study investigated the odor-associated bacterial community in automobile HVAC systems. Through a metagenome analysis, it was found that; Massilia (42.426%), Sphingomonas (28.200%), (10.780%), and Methylobacterium (5.756%) were abundant in the HVAC systems. Massilia can cause the biodegradation of polycyclic aromatic hydrocarbons (PAHs) producing odor in automobiles. Sphingomonas produces volatile halogenated compounds or degrades organic pollutants. Rhodococcus is reported to produce sulfur compounds which give off an odor similar to rotting eggs and cabbages. Methylobacterium is one of the most representative bacteria that causes odor in automobile HVAC systems. The evaporator is considered as the appropriate habitat for microorganisms in automobiles because of its high humidity and organic adsorption. Massilia, Sphingomonas, Rhodococcus, Methylobacterium, Bacillus, Staphylococcus, Arthrobacter, Micrococcus, and Pseudomonas, listed in order from most to least present, were isolated as abundant bacteria in the evaporator of the HVAC systems.

본 연구는 2011년 3월부터 2012년 5월까지 부영양수 계에 네 가지 여재(스펀지, 화산석, 활성탄, 수산화마그네 슘)를 사용한 부유습지를 설치하여 장기적인 수질변화를 조사하였다. 또한 부유습지에 유입되는 유입수와 네 가지 여재를 거쳐 최종적으로 통과된 유출수내의 식물플랑크 톤과 동물플랑크톤 군집을 분석하였다. 실험기간 동안 탁 도(66%), SS (79%), Chl-a (80%), COD (24%)의 농도는 유 입수에 비해 유출수에서 뚜렷하게 높은 감소율을 나타냈 다(p⁄0.001). NO2-N와 NH3-N은 각각 24%, 20%로 유 의한 감소를 나타냈으나(p⁄0.05), NO3-N와 TN은 평균 10% 미만의 제거율을 나타내었다(p¤0.05). 한편 PO4-P 와 TP는 모두 평균 65% 이상의 제거율을 나타냈다(p⁄ 0.01). 또한 식물플랑크톤밀도는 유출수에서 유입수보다 2.6배 정도 낮게 나타낸 반면, 동물플랑크톤은 유입수보 다 유출수에서 평균 3.5배 이상 높은 밀도를 나타내었다. 본 연구에서 네 가지 여재를 이용한 수질개선용 부유습 지는 특히, 입자성 물질(SS, Chl-a, COD, TP)과 용존성 영양염(NO2-N, NH3-N, PO4-P)에 대하여 높은 제거율을 나타내어, 결과적으로 부영양 호소에서 수질개선 및 조류 저감에 대한 적용가능성을 보여주었다.