서해안 및 도서지역의 상록활엽수림 복원을 위한 기초조사로서 상록활엽수림의 복원과정을 지속적으로 모니터링하기 위하여 천리포수목원 닭섬에 대한 현존식생구조를 밝히고 도입식물들의 활착 상태를 조사하였다. 천리포수목원의 한랭지수는 -7.7℃.month로 목포(-7.6℃.month)와 거의 비슷하였으며, 서해안을 흐르는 황해난류의 영향으로 한서의 차가 비교적 적은 난온대성 기후특성을 나타내고 있다. 11개 식생구조구에 대한 식생조사 결과 곰솔이 우점종이었으며 1976년부터 식재하기 시작한 상록활엽수는 26종 708개체이었으며, 이 중 15종 121개체가 활착하여 약 19.1%의 생존율을 나타냈다.

Background : The 1,2-unsaturated PAs, reported to be widely present in medicinal plants belonging to Asteraceae, Boraginaceae, and Fabaceae, cause hepatotoxicity and genotoxicity in humans and animals. Hence, there is a need for an analytical method that allows these dangerous plant toxins to be determined. In this study, we developed a method that can be used for the rapid and accurate determination of nine toxic PAs in medicinal plants using ultra-pressure liquid chromatography–electrospray ionization–quadrupole–time-of-flight mass spectrometry (UPLC-ESI-Q-TOF). Methods and Results : The compounds were eluted onto a C18 column with 0.1% formic acid and acetonitrile, and separated with good resolution within 11 min. all analytes was characterized by its precursor ions generated by ESI-Q-TOF and fragment ions produced by collision-induced dissociation (CID), which were used as a reliable database. The proposed analytical method was verified with reference to the ICH guidelines. The proposed UPLC-ESI-Q-TOF method was applied to four medicinal plants, and lycopsamin, echimidine, senkirkine and senecionine were detected by matching with reference standard, and additional six PAs were tentatively identified though chemical profiling. In addition, the QuEchERS method was the most efficient in comparison with methods like hot water and methanol in extraction efficiency of pyrrolizidine alkaloids. Conclusion : The our proposed method can determine PAs rapidly and accurately in medicinal plants and will be utilize as an important data for other researchers who need analytical information of PAs.