The surface of magnetite () nanoparticles prepared by coprecipitation method was modified by carboxylic acid group of poly(3-thiophenacetic acid (3TA)) and meso-2,3-dimercaptosuccinic acid (DMSA). Then the lysozyme protein was immobilized on the carboxylic acid group of the modification of the magnetite nanoparticles. The magnetite nanoparticles are spherical and the particle size is approximately 10 nm. We measured quantitative dispersion state by dispersion stability analyzer for each nanoparticles with and without surface modification. The concentration of lysozyme on the modified magnetite nanoparticles was also investigated by a UV-Vis spectrometer and compared to that of magnetite nanoparticles without surface modification. The functionalized magnetite particles had higher enzymatic capacity and dispersion stability than non-functionalized magnetite nanoparticles