In this study, the removal performance of high-concentration H2S and complex odors was evaluated for bio-filters installed in sewage treatment plants and manure treatment plants. The amount of odor generation according to temperature was found to be higher in summer than in spring. It was found that the longer the empty bed retention time of the bio-filter, the higher the odor removal efficiency. Therefore, in order to effectively remove odors, it is necessary to maintain a sufficiently long residence time when designing a bio-filter. In addition, a case in which a bio-filter and a wet pre-treatment system were combined to remove high-concentration odors was studied. The result showed that the wet pre-treatment was effective in removing high-concentration odors. In particular, most of the hydrogen sulfide could be adequately removed by wet pre-treatment.