Lilium속 식물들에서 화색 생합성조절 유전자의 대 사경로 규명을 위해 화학적 및 생화학적 방법을 이 용하여 백합의 flavonol과 anthocyanidin 색소의 생 성여부를 분석하였다. flavonol은 L. longiflorum과 11개의 다른 품종에서 kaempferol(Km)과 quercetin (Qu)의 형태로 spectrophotometer에 의해 검출되었 다. Anthocyanidin 색소로는 ‘Le Reve’, ‘Etude’, ‘Acapulco’, ‘Star Gazer’, ‘Montreux’, ‘Monte Negro’ 에서 cyanidin이 검출되었고, 나머지 품종인 ‘Siberia’, ‘Royal Lace’, ‘Nove Cento’, ‘Elite’, ‘Cannes’에서는 anthocyanidin이 검출되지 않았다. 즉 이들 5개 품종 은 DFR효소 활성이 없는 acyanic 품종인 것으로 나 타났다.

In this work, we analyzed the activity of control enzymes of flower color biosynthesis, chalcone synthase (CHS) and flavanone 3-hydroxylase (FHT) using biochemical and enzymological methods in Lilium longiflorum and 11 Lilium cultivars. The results obtained are as follows ; Naringenin (NAR) was synthesized in all Lilium cultivars tested by the catalytic activity of CHS which used malonyl-CoA and 4-coumaryol-CoA as substrates. Substrate-specific activity of CHS was observed because eridictiol (ERI), which uses caffeoyl-CoA as a substrate, was not detected in tested cultivars. In next step, dihydroflavone product was synthesized by FHT using flavanones as a substrate. FHT synthesized dihydrokaempferol (DHK) by using NAR as substrates. A remarkable activity of FHT was observed in other 11 cultivars.