The effect of the Penicillium sp. PS113 with high phosphate solubilizing activity and Lactobacillus sp. was studied on cucumber growing test in green house. Inoculation of Penicillium sp. PS113 5.0×10⁶cfu/㎖ and Lactobacillus sp. 6×10⁶cfu/㎖ were longer than control about 3cm length of cucumber. The average weight was also increased 89.2g more than control. Total product of cucumber was 10,681.7g and it was increased 4517.3g as compared to control. Thus, Inoculations of high phosphate solubilizing microorganism increased in productivity on cucumber.