참다슬기 아가미 조직으로부터 heat shock protein 70 유전자를 분리 · 동정하였다. 참다슬기 HSP70 cDNA의 open reading frame (ORF)는 1,917 bp로 639개의 아미노산을 암호화하여 분자 량은 약 70 kDa으로 예측되었다. 생물정보학 배열분석에 의해 HSP 유전자 기능과 관여되어 있는 3가지 주요 signature motifs와 보존된 도메인을 확인하였다. 계통학적 분석을 통하여 참 다슬기 HSP70 유전자는 왕우렁이 Pomacea canaliculate와 같은 클러스트에 포함된다는 사실을 확인하였다. 수온 및 염분 변화에 따라, 참다슬기 HSP70 mRNA 유전자 레벨은 유의적으로 증 가하였으며(p < 0.05), 이는 외부자극요인을 파악할 있는 분자생물학적 마커로서 활용될 수 있 을 것으로 사료된다.

During the last two decades, many industrial complexes for heavy and chemical industries have been established along the Korean coastline, thereby increasing the pollution materials burden on the coastal environment of seawater. Masan Bay is one of the most polluted coastal areas in Korea and the main soures of pollutants are domestic and industrial wastewater from Masan, Changwon. This study was aimed to evaluate relationships among the physicochemical parameters in the bottom water of Masan bay and to examine environmental factors affecting to pollutions of seawater by factor analysis. The factor loading, 1 is showed higher increasing inclination after 1989 year in station 1. The variance of pollutant materials is showed 43.7% in which the coastal inflow water is indicated external loadings(factor 1 : NO3- -N, TN, factor 4 : SiO2-Si) corresponded to domestic sewage, industrial wastewater, and earth-sands in the bottom water of Masan bay. And the internal loadings(factor 2 : SS, salinity, factor 3 : W.T., DO) are explained 33.8% corresponded the phenomena of sedimentary layer and oxygen concentration. Therefore, The external loadings are explained by the higher factor pollutantal variance in Masan bay.