We collected 32 maize inbred lines from eastern cereal and oilseed research center in Canada to develop new maize varieties. We also evaluated genetic diversity, genetic relationships, and population structure using 35 SSR markers. A total of 269 alleles were revealed in 35 loci with an average of 7.69 and a range between 3 and 15 alleles per locus. The genetic diversity values varied from 0.176 to 0.889 with an average of 0.691. The polymorphic information content varied from 0.171 to 0.879 with an average of 0.659. Population structure analysis indicated that 32 Canadian maize inbred lines comprised four major groups and one admixed group based on a membership probability threshold of 0.80. The four major groups contained 13, 2, 5 and 2 maize inbred lines, respectively. From genetic relationships analysis, the all inbred lines were divided into three main groups at 26% genetic similarity. Group I included 22 inbred lines, and Group II included 9 inbred lines. Group III consist of only one inbred line. The results in this study would be useful for the improvement and development of new cultivars, planning crosses for hybrids or development of inbred line in maize breeding program
In order to develop a core set and new corn variety in Korea, we evaluated the morphological characteristics of 194 maize accessions by examining eight quantitative characteristics. On the evaluation of quantitative traits for 194 maize accessions, they showed the morphological variations in tassel length (35.1±5.0 cm), plant height (226.1±33.7 cm), ear height (86.3±22.6 cm), stem diameter (2.3±0.6 cm), leaf width (9.3±1.1 cm), ear length (14.5±2.4 cm), ear row number (14.1±1.9 row), and 100 kernel weight (24.9±4.4 g). The results of principal component analysis (PCA) indicated that the tassel length, plant height, and ear height greatly contributed to positive direction on the first principal component axis. One-hundred kernel weight contributed to negative direction on the second principal component axis. Thus these morphological characteristics, which contributed greatly in the first and second principal components, might be useful for discrimination among 194 maize accessions. In our study, seven accessions, such as IT026357, IT026441, IT027321, IT033271, IT033591, IT033597 and IT124273, particularly were measured high on yield-related traits. Consequently, the 194 maize accessions used in this study could be used as promising materials for maize breeding programs such as development of new hybrid in Korea.