For the last few decades, much research has been tried to improve crop’s characteristics such as crop yield, quality, seeding period and environmental adaptability. In this paper, the effect of nanobubbles on the germination rate of barley seed is experimentally investigated. The air nanobubble was generated in water by gas-liquid mixing method. The results were shown that the mean diameter and concentration of nanobubble fabricated in DI water are 133 ㎚ and 8.59✕108 particles/㎖, respectively. Also, the seed germination rate for the nanobubble water was approximately 40 % higher than that of pure water.