This study were examined whether plasminogen activators (PAs) are produced by porcine fresh or frozen-thawed cumulus-oocytes complexes (COCs) and cumulus cell free-oocytes. In fresh or frozen-thawed COCs and oocytes for 0 hour cultured, no activity of PAs was detected. However, at 24 hours of culture urokinase-type plasminogen activator (uPA) was detected in COCs and denuded oocytes. In the frozen-thawed COCs and cumulus cell free-oocytes cultured for 24 hours, no PAs were observed. After COCs were cultured for 48 hours, tissue-type plasminogen activator (tPA) and tPA-PAI were observed in COCs only. In the frozen-thawed COCs and cumulus cell free-oocytes cultured for 48 hours, no PAs were observed. These results suggest that uPA, tPA and tPA-PAI are produced by porcine COCs, but only uPA by oocytes during maturation for 24 hours. Only tPA, and tPA-PAI are produced by COCs cultured for 48 hours, and no PAs are produced by denuded-oocytes cultured for 48 hours. In all of the frozen-thawed groups, no PAs are observed by COCs and denuded-oocytes.

In recent years, electronic products have played an increasingly important role in modern society and have penetrated into people’s life and work. With the continuous development of technology and the increasing demand for convenience and innovation, the functions and performance of electronic products continue to improve. However, the intensification of market competition and the rapid advancement of technological progress have brought many challenges and opportunities to the field of electronic products. This paper takes the new product development project management process of Lenovo Company as the research object, adopts investigation analysis method, literature analysis method and project management theory and gateway development management theory to deeply analyze the existing problems in the new product development project management of Lenovo Company, such as: The new product development cycle is long, the new product development cost is over budget, the new product development project organization and coordination ability is weak, and the project monitoring and feedback are not timely. The causes of these problems are analyzed. In view of the existing problems, this paper makes an in-depth study of the relevant theories of project management and new product development, and puts forward an optimization scheme suitable for the improvement of the new product development project management of Lenovo Company. It means adopting agile development scheme, establishing project development cost control point, optimizing organizational structure, and establishing perfect project management supervision and feedback mechanism. Finally, based on the current situation of Lenovo ‘s new product development, this paper proposes the guarantee measures for the smooth implementation of the new product development project management optimization plan, namely, talent guarantee, development and innovation awareness, risk control awareness, and management awareness in strengthening cultural construction, so as to ensure the smooth implementation of new product development project management.

As a method to reduce collision accidents of ships at sea, this paper suggests an expert system for collision avoidance and navigation (hereafter "ESCAN"). The ESCAN is designed and developed by using the theory and technology of expert system and based on the information provided by AIS and RADAR/ARPA system. In this paper the ESCAN is composed of four(4) components; Facts/Data Base in charge of preserving data from navigational equipment, Knowledge Base storing production rules of the ESCAN, Inference Engine deciding which rules are satisfied by facts or objects, User System Interface for communication between users and ESCAN. The ESCAN has the function of real--time analysis and judgment of various encountering situations between own ship and targets, and is to provide navigators with appropriate plans of collision avoidance and additional advice and recommendation This paper, as a basic study, is to introduce the basic design and function of ESCAN.

Evaluation of collision risk plays a key role in developing the expert system of navigation and collision avoidance. This paper presents a new collision risk model formula that is one modification model on the basis of one approach to the evaluation of collision risk using sech function produced in earlier studies. And as a tool of the evaluation field of ship collision, this paper applied the new model in appraising the collision risk and represented how to decide the safe range of own ship's action. Moreover this paper also analyzed theoretically how to determine the coefficients as described in the new modification model, and suggested the appropriate values as applicable.