본 실험은 곡류 작물중에서 육종의 소재로써 많은 장점을 지닌 호밀을 Gimsa C-분염법과 FISH기법을 이용하여 구성이질 염색질과 5S와 18S-26S rRNA 유전자의 염색체상의 위치를 확인하고자 본 실험을 수행하였다. 그 결과를 요약하면 아래와 같다. 1. C-분염의 분석 결과 7쌍의 호밀의 염색체 중에서 1, 2, 3과 7번 염색체는 중부염색체 이었고, 4, 5과 6번 염색체는 차중부염색체 이었다. 1번 염색체는 2차 협착부위로 동원체를 지니고

The 5S rRNA gene is considered as valuable resource for chromosome landmarks and evolutionary studies. In this study, the tandem repeat unit of 5S rRNA gene containing the coding sequence and non‐transcribed spacer (NTS) region was amplified by PCR, cloned, and sequenced from Allium wakegi (2n=XY=16) and the two ancestors, A. cepa and A. fistulosum. Although there were intraspecific variations among the clones of each species, we could construct each consensus sequences for A. cepa and A. fistulosum and two consensus sequences for A. wakegi. The sequence of the 120 bp coding region was completely homologous among the consensus sequences from the Allium species examined. However, the sequence in the NTS region was significantly variable and informative in genome analysis. The variations were found to be clustered between the positions 230 and 269, and also distributed broadly as single nucleotide. A. wakegi could be divided into two classes by the 5S rRNA sequences (AWAK‐1 and AWAK‐2). AWAK‐1 showed high homology to the genome of A. cepa and AWAK‐2 to that of A. fistulosum. Fluorescence in situ hybridization technique was applied to analyze the distribution of 5S gene loci. In A. wakegi, 5S rRNA sequences were detected in two different chromosomes, each one showing the pattern identical to the chromosome donated from each genome of both ancestors.

In order to develop molecular markers related to stay green phenotype, AFLP analysis was conducted using near-isogenic lines for either stay green or non stay green trait. Both lines have characteristics of multi-ear and tillers (MET). Two out of 64 primer combinations of selective amplification identified three reproducible polymorphic fragments in MET corn with stay green. Both of E+AGC/M+CAC and E+AAG/M+CAA primer combinations produced two and one specific polymorphic fragments linked to stay green trait, respectively. For the conversion of AFLPs to sequence tag sites (STSs), primers were designed form both end sequences of each two polymorphic fragments. One fragment, which was amplified with E+AAG/M+CAA primer combinations, possessed 298 bp long and showed a 91~% homology with maize retrotransposon Cinful-l. One out of two polymorphic fragments produced with E+AGC/M+CAC primer combination had 236 bp long and matched a 96~% homology with an intron region of 22kDa alpha zein gene cluster in Zea mays. One out of two PCR fragments amplified with MET2 primer set in the stay green MET was not produced in the non-stay green MET. The developed AFLP and STS marker could be used as an efficient tool for selection of the stay green trait in the MET inbred.

Rye genome- and chromosome-specific DNA markers were selected to easily identify the existence of rye chromatin in the wheat genome by RAPD analysis. Among 260 decamer primers used, five primers, namely, OPC01, OPF07, OPF11, OPH09, and OPH16 amplified rye