The toxicity of Kaempferia galanga rhizome-derived methanol extract (RME), powder (RP) and steam distillate (RSD) to Meloidogyne incognita second-stage juveniles (J2) and eggs and their effects on Lycopersicon esculentum germination and growth were examined in vitro and in pot experiments. Results were compared with those of three nematicides. In contact+fumigant bioassays with J2, RME applied at 1, 0.5 and 0.25mg/g soil exhibited 92, 88, and 73% mortality, respectively. The lethality of RME was almost similar to that of carbofuran but lower than that of either fosthiazate or metam-sodium. RSD and RP were less active than RME. In vapor-phase mortality bioassayswith J2, the test materials were effective in closed container than in open one, indicating that mode of delivery was, in part, a result of vapor action. RME, RSD, and fosthiazate treatments resulted in 91, 100, and 95% inhibition of egg hatch at 250μg/ml and 82, 88, and 81% inhibition of egg hatch at 100μg/ml, respectively. In filter-paper bioassays with L. esculentum seed at 8.8μg/cm2, RME and RP did not cause germination inhibition, while RSD and fosthiazate treatments resulted in 84 and 13% germination inhibition. In pot tests, RME and RSD applied at 8mg/g soil reduced galling caused by M. incognita significantly and fosthiazate at 0.02mg/g soil reduced galling completely. Rhizome materials did not cause any adverse effect on growth of L. esculentum, while fosthiazate application caused significantly reduced root weight. K. galanga rhizome-derived materials, particularly methanol extract, merit further study as potential nematicides and hatching inhibitors for the control of M. incognita populations as fumigants with contact action.

The nematicidal activity of Kaempferia galanga rhizome-derived materials (methanol extract, steam distillate, and powder) toward M. incognita second-stage juveniles (J2) was examined using residual contact toxicity and pot bioassays. In residual contact toxicity bioassays with J2, the active principles of K. galanga rhizome were identified as the phenylpropanoids ethyl cinnamate (EC, 1) and ethyl-p-methoxycinnamate (EMC, 2) by spectroscopic analysis. EC exhibited 95% and 49% mortality at 0.06 and 0.04 mg/ml toward J2, respectively, whereas EMC showed 98% and 63% mortality at 0.1 and 0.08 mg/ml. In pot tests with J2, K. galanga rhizome methanol extract gave 92% and 82% mortality at 100 and 20 mg/50g soil, respectively. Steam distillate gave 88% and 68% mortality at 85 and 42.5 mg/50g soil, respectively, whereas rhizome powder provided 83% and 62% mortality at 400 and 200 mg/50g soil. K. galanga rhizome-derived materials, particularly EC and EMC, merit further study as potential root-knot nematode control agents or leads because of their great activity as a nematicide.

차나무뿌리썩이선충(Pratylenchus loosi)이 국내에서 처음으로 전라남도 명암군과 제주도 남제주군의 차나무 뿌리와 주변 중에서 발견되었다. 차나무뿌리썩이선충의 두부주름은 2개이며 암컷의 길이는 433-646이고, a ＝ 29.1-37.5, b ＝ 5.1-6.4, c ＝ 15.0-21.3, vulva (%) ＝ 73.0-85.4였다. 수컷의 몸길이는 408-512 였으며 a ＝ 36.1-40.0, b ＝ 4.8-6.7, c ＝ 17.0-19.0, spicule ＝ 14.1-18.0였다. 구침의 길이는 11.6-18.0였으며, 측선의 갯수는 4개였다. 저정낭은 정자로 차 있으며, 모양은 둥근 고치모양 또는 직사각형 모양이다. 꼬리의 형태는 가늘고 끝이 둥글거나 뾰족하다.

농과원 곤충과 선충실에 보관되어있는 식물기생충 표본들과 경북대학교 농과대학 농생물학과 선충연구실의 표본을 정리하던 중 한국 미기록종, Pratylenchoides utahensis가 동정되어 형태적 특징에 관하여 기재한다.