Mycoplasma (M.) felis and M. canis is related with pneumonia or conjunctivitis in domestic cats and several diseases in a variety of other animals, including lower respiratory tract disease or pleuritis. Polymerase chain reaction (PCR) assays has been reported as an easy and useful method. It could be conducted even on nasal swab samples as a non-invasive rapid testing tools for large numbers of Mycoplasma species. However, PCR assays have to conduct multiple assays because of a lot of Mycoplasma species. Therefore, it need to perform several tests and reveal time consuming procedures. In this study, we developed a sensitive and specific multiplex polymerase chain reaction (PCR) assay that detects simultaneously two species like as M. felis and M. canis. The simultaneous detection of M. felis and M. canis primers were used to differentiate two mycoplasma species. The target DNA fragments were specifically amplified M. felis and M. canis PCR with 16S ribosomal DNA primers. Single and mixed Mycoplasma species DNA templates were submitted to validate the specificity of the multiplex PCR. The corresponding specific DNA products were amplified for each pathogen. The detection limit of the developed multiplex PCR is 102 pg with M. felis and M. canis DNA. Furthermore, the developed multiplex PCR detected successfully M. felis in feline nasal specimens. The multiplex PCR assay provides a novel tool for simultaneous detection and differentiation of M. felis and M. canis in cats.

Animal-Assisted Intervention programs and interventions with students are becoming promising field in school and therapeutic settings. Following this trend, Animal-Assisted Intervention (AAI) program was designed to help students adopt more easily to school life and to encourage them to keep healthy relationships with others. Accordingly, the purpose of this study was to identify the effects of animal-assisted intervention (AAI) on the middle school students' sociability and depression. The participants in this study, 8 middle school students residing in Seoul who were interested in animals and agreed to the research attended this program. This study was conducted two times a week for 6 weeks (a total of 12 times) with one main researcher and three research assistants. The major research tool was the test of sociability and depression and the statistical test being used for this study was Wilcoxon's signed ranks. The results were as follows. First, the study showed the significant effects of animal-assisted intervention on sociability for middle school students. There were significant differences in many aspects such as reliability, autonomy, sociability and compliance. Second, significant reductions in anxiety and depression were found after the introduction of animal-assisted intervention for students. Students who participated this program experienced a reduction in depression and stress to some degree. Also, they began to form much more positive friendships and followed school rules as well. Based on the above results, it can be seen that animal-assisted intervention program significantly has the positive effects on the social adaptability and depression of the middle school students. Therefore, this animal-assisted intervention should be considered as a positive program for emotional and psychological support for middle school students.

본 연구는 동물매개중재프로그램이 중학생의 정서지능과 사회성에 미치는 영향을 검증하고자 하였다. 연구대상은 서울특별시 A구 B소재의 중학교에서 방과 후 교육을 이용하는 학생 중에 동물에 관심이 있는 남학생들로 프로그램연구에 동의하는 8명을 선정하였다. 동물매개중재프로그램은 주 2회 45분씩 6주간 총 12회기를 실시하였으며 프로그램계획 및 운영은 동물매개심리상담사 1급자격증을 취득한 본 연구자와 보조 진행자로 한국동물 매개심리치료학회 소속 동물매개심리상담전문가 1인과 펫파트너로 동물매개심리치료학 전공 4학기생과 동물매개심리상담사 2급자격증을 가진 2인이 반려견 2두와 함께 참여하였다. 치료도우미견을 활용한 동물매개중재프로그램의 효과성을 측정하기 위해 사용한 검사도구는 정서지능 척도검사, 사회성 척도검사이며 비모수적 통계분석 (non-parametrictest)방법인 윌콕슨 부호순위검증(Wilcoxon's signed ranks test)을 통 해 사전검사와 사후검사간의 점수 차이를 분석하였다. 본 연구결과 동물매개중재 프로그 램이 중학생의 정서지능과 사회성에 영향을 미치는 것으로 나타났다. 먼저 정서지능에서 정서인식 및 표현, 감정이입, 정서·사고촉진, 정서활용 그리고 정서조절에서 유의한 차이 가 나타났고, 사회성에서는 신뢰성, 자율성, 사교성 그리고 준법성에서 유의한 차이가 나타났다. 특히 정서조절과 사교성에서 다소 높은 효과를 나타내었다. 이는 동물매개중재의 차별화된 장점의 결과로 보인다. 따라서 동물매개중재프로그램은 중학생의 정서지능과 사 회성 향상에 효과가 있다는 연구결과의 의의가 있다.

A cytosolic ascorbate peroxidase, hydrogen peroxide-scavenging enzyme, was characterized from Codonopsis lanceolata. The cytosolic ascorbate peroxidase cDNA (CAPX) was 983 nucleotides long and possess an open reading frame of 753 bp with 251 amino acids (MW 27.9 kDa) with pI 5.61. The deduced amino acid sequence of CAPX shows high homology to other known cytosolic APXs (78~83%), but the CAPX was clustered independently from compared ten plant APXs. The CAPX gene was highly expressed in leaf and stem tissues, but not in root. When Codonopsis leaves cut using scalpel were soaked in 1 mM hydorgen peroxide, the expression of CAPX gene was suppressed.

A cinnamoyl CoA reductase (CCR) cDNA (ClCCR) was isolated from tabroot mRNAs of Codonopsis lanceolata by cDNA library construction, and its expression was investigated in relation to abiotic stresses. The ClCCR is 1008 bp in length with an open reading frame (ORF) of 336 amino acids. The deduced amino acid sequence was showed high similarity with cinnamoyl-CoA reductases of P. tremuloides (AAF43141) 87%, F.×aranassa (AAP46143) 83%, L. album (CAD29427) 80%, E. gunnii (CAA66063) 72%, S. tuberosum (AAN71761) 83%. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis was revealed that the ClCCR expression was regulated by abiotic stresses.