Ion exchange resins are commonly employed in the treatment of liquid radioactive waste generated in nuclear power plants (NPP). The ion exchange resin used in NPP is a mixed-bed ion exchange resin known as IRN-150, which is of nuclear grade. This resin is a mixture of cation exchange resin and anion exchange resin. The cation exchange resin removes cationic radionuclides such as Cs and Co, while anion exchange resin handles anions (e.g., H14CO3 -), effectively purifying the liquid waste. Spent ion exchange resins (spent resin) containing C-14 are classified as low and intermediate level radioactive waste, and their radioactivity needs to be reduced as it exceeds the disposal limit regulated by law. Therefore, the microwave technology for the removal of C-14 from spent resin has been investigated. Previous studies have successfully developed a method for the effective removal of C-14 during the resin treatment process. However, it was observed that, in this process, functional groups in the resin were also removed, resulting in the generation of off-gases containing trimethylamine. These off-gases can dissolve in water from process, increasing its pH, which can subsequently hinder the recovery of C-14. In this study, we investigated the high-purity recovery of C-14 by adjusting the moisture content within the reactor following microwave treatment. Mock spent resins, consisting of 100 g of resin with HCO3 - ion-exchanged and 0, 25, or 50 g of deionized water, were subjected to microwave treatment for 40 or 60 minutes. Subsequently, the C-14 desorption efficiency of the mock spent resins was evaluated using an acid stripping process with H3PO4 solution. The functional group status of the mock spent resins was analyzed using 15N NMR spectroscopy. The results showed that the mock spent resins exhibited efficient C-14 recovery without significant functional group degradation. The highest C-14 desorption efficiency was achieved when 25 g of deionized water was used during microwave treatment.