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        검색결과 1

        1.
        2014.07 서비스 종료(열람 제한)
        The White backed planthopper (WBPH), Sogatella furcifera (Horvath) is one of the serious insect pests in rice growing region in Asia. When rice is attacked by the insect it releases secondary metabolites for self-defense. In this study, we identified WBPH-mediated compounds from a cross ‘Cheongcheongbyeo/Nagdongbyeo’ doubled haploid (CNDH). The compounds were located in chromosome. Leaves and stem of CNDH lines were infected by 2∼3 insta of 3 weeks WBPH and samples were extracted by 90% methanol. Extracted compounds were analyzed through HPLC. TLC was used in separating the target compounds. QTL analysis of compound was done using winQTLcart 2.5 program. Chrysoeriol was highly contained in Cheongcheongbyeo. QTL location is found on chromosome by winQTLcart 2.5. QTL analcited with compound7 was detected on chromosome 4, 7 and 12. qFla4 was detected on chromosome 4 in RM280-RM6909 at LOD 3.5 with 30% of variation. qFla7 was detected on chromosome 7 in RM248-RM1134 with LOD 3.0 with 30% of variation. qFla12 was detected on chromosome 12 in RM1226-RM12 with LOD 2.7 with 40% of variation. Cochlioquinone was detected on chromosome8, qFla8 in RM23230-RM3689 with LOD 2.5 with 30% of variation. Chrysoeriol and Cochlioquinone separated to condition of (Chloroform: Methanol:1-Butanol:Water=4:5:6:4). Separated compounds were analyzed by LC/MS and NMR. These results, investigation is being done to ditermine how the secondary metabolites come lead to pathways of genes and its effect on WBPH relation.