강원지역에 발생하는 돌발해충은 미국선녀벌레, 갈색날개매미충, 꽃매미 등이 있다. 이러한 돌발해충의 월동은 동계최저기온에 의해 가장 큰 영향을 받게 된다. 따라서 꽃매미는 2012년부터 갈색날개매미충은 2016년부터 월동난괴 를 채집하여 동계최저기온에 따른 부화율을 측정하여, 동계최저기온과 돌발해충의 부화율 관계에 대한 조사를 실시하였다. 동계최저기온이 –11℃이하인 날이 35일 이상 지속된 2012년과 2017년 겨울은 꽃매미의 부화율이 9.2%, 7.2%로 낮게 나타난 반면, -11℃이하인 날이 16일로 비교적 낮은 경과일수를 보인 2013년 겨울에는 75.5%의 부화율을 보였다. 갈색날개매미충 역시 –11℃이하인 날이 35일 이상이었던 2017년 겨울에 43.1%의 낮은 부화율을 보였지만 20일로 적은 경과일수를 보인 2016년 겨울에는 89.4%로 약 2배정도 높은 부화율을 확인할 수 있었다. 이는 일정수준 이상 동계최저기온을 경과한 돌발해충의 월동난은 성공적인 월동을 하지 못한다는 것을 의미한다.

연 10회 이상 발생하는 온실가루이는 토마토 재배 시 6월 하순 기온이 상승하면서부터 발생량이 급격히 증가하고 약제에 대한 저항성이 강해 많은 농가에서 방제에 많은 어려움을 겪고 있다. 이러한 온실가루이의 효과적인 방제를 위해 최적교호살포 약제조합을 선발하는 실험을 실시하였다. 작용기작에 따라 약제는 각각 신경전달기능을 억제하고, 키틴생합성을 저해하며, 지질생합성을 저해하는 작용기작을 가진 약제로 선정하였다. 각 처리구별로 약제 살포순서를 다르게 하여 교호살포한 결과 모든 처리구에서 3회 약제처리 후 성충밀도가 처리전 밀도보다 유의한 차로 줄어들었다. 약충에 있어서는 약제처리전 모든 처리구에서 약충 발생이 낮아 정확한 밀도억제효과를 확인하기 어려웠지만 최종조사에서는 신경전달저해, 탈피저해, 지질생합성저해 약제 순으로 처리한 처리구의 밀도가 무처리구와 비교하였을 때 일정수준 억제되는것을 확인하였다

The White backed planthopper (WBPH), Sogatella furcifera (Horvath) is one of the serious insect pests in rice growing region in Asia. When rice is attacked by the insect it releases secondary metabolites for self-defense. In this study, we identified WBPH-mediated compounds from a cross ‘Cheongcheongbyeo/Nagdongbyeo’ doubled haploid (CNDH). The compounds were located in chromosome. Leaves and stem of CNDH lines were infected by 2∼3 insta of 3 weeks WBPH and samples were extracted by 90% methanol. Extracted compounds were analyzed through HPLC. TLC was used in separating the target compounds. QTL analysis of compound was done using winQTLcart 2.5 program. Chrysoeriol was highly contained in Cheongcheongbyeo. QTL location is found on chromosome by winQTLcart 2.5. QTL analcited with compound7 was detected on chromosome 4, 7 and 12. qFla4 was detected on chromosome 4 in RM280-RM6909 at LOD 3.5 with 30% of variation. qFla7 was detected on chromosome 7 in RM248-RM1134 with LOD 3.0 with 30% of variation. qFla12 was detected on chromosome 12 in RM1226-RM12 with LOD 2.7 with 40% of variation. Cochlioquinone was detected on chromosome8, qFla8 in RM23230-RM3689 with LOD 2.5 with 30% of variation. Chrysoeriol and Cochlioquinone separated to condition of (Chloroform: Methanol:1-Butanol:Water=4:5:6:4). Separated compounds were analyzed by LC/MS and NMR. These results, investigation is being done to ditermine how the secondary metabolites come lead to pathways of genes and its effect on WBPH relation.

Genetically modified (GM) plant claims to be the solution to global poverty, and potentially solving environmental change and food requirement by increased human population. In this study, we were evaluating agronomic characteristics and chemical properties of two GM drought-tolerant rice (CaMsrB2-8 and CaMsrB2-23) compared with donor cultivars (Ilmi). Statistical analysis agronomic characteristics GM and donor rice showed no significant difference between both of them. Yield and appearance of rice grain, GM rice was a similar to the donor rice. Chemical composition analysis showed that GM drought-tolerant rice has no different with donor rice. This result indicated that GM drought tolerant rice has no big significant difference agronomic character and chemical properties; it can be solve food shortages in spite of drought condition.

This experiment conducted to identify the changes of the response when white-back planthopper (WBPH, Sogatella furcifera), were inoculated in 10 days rice leaves after germination. We confirmed the difference between inoculated and uninfected plants by in the different time period (1day, 1 week, 2 weeks, and 3 weeks after inoculation). Breeding rice and WBPH maintained at 26∼28℃ with 60 % humidity. 3 leaves plants (TN1, Cheongcheong, and Nagdong) were inoculated with 2∼3 instars WBPH. Harvested rice plant samples were completely dried in dark condition and then samples were completely immersed in a solution of methanol for 3 days under darkness. Dissolving in water and then de-fatted three times with hexane. 100 ppm samples were applied to HPLC, eluting with acetonitrile and 0.1 % acetic acid by C18 (5ph column Agilant) and detected at 254 nm. We confirmed the difference of peak using LC/MS/MS (API-2000). The results showed that three weeks from the day of inoculation was increased at the molecular weight 118.1, 264.2 and 364.2.

Quantitative trait locus (QTL) mapping is a highly effective approach for studying genetically complex forms of plant shattering. With QTLs mapping, the shattering loci can be described. SSR marker is based on the imformation of Simple Sequence Repeat and easy to analyze using PCR and has high reproducibility. For analyzing QTLs associated with shattering, we selected 219 SSR markers from 254 SSR markers and used them for implementing Mapmaker(Ver. 3.0) and Mapchart(Ver. 2.2). Mapmaker help to calculate distances between each markers and Mapchart is a program for drawing Genetic map. This Genetic map of rice (Oryza sativa L.) covering 2082.4 cM with 9.5 cM between makers in the Kosambi function has been constructed using 120 F1 DH plants from a single cross between the indica variety Chungchung and the japonica variety Nagdong.