In the past studies of Lyophlium shimeji, it was reported that the quantity of sufficient starch used as a carbon source was able to supply the factor that allows successful fruit-body formation without raising osmotic pressure in the medium. Glucoamylase are exo Glucosyl hydrolase, which catalyze the release glucose from the nonreducing ends of amylose, amylopectin, and other polysaccharides. Glucoamylase genes are found in many prokaryotic and eukaryotic microbes that use starch as a carbon source. It was believed to be important in the utilization of starch by the basidiomycetous fungus. Glucoamylase activity in the medium increased markedly during fruit-body formation. So study of the characteristic of glucoamylase in Pholiota nameko will provide the basis for P .nameko fruit body formation. In this research, in order to confirm the presence of glucoamylase gene in P. nameko genome, the genomic DNA was prepared from P. nameko NGW19-6 strain and was used as template to amplify the glucoamylases gene (PnGlu1). To prepare genomic DNA from the P. nameko NGW19-6 strain, the mycelium was grown on 10 ml of PD liquid medium (potato 200 g/l ,Glucose 20 g/l) prepared with tap water in a 100 ml Erlenmeyer flask and at 25°C for 7 days. Genomic DNA fragment encoding the glucoamylase protein (PnGlu1) were amplified by PCR with degenerate primer F15-GP2-AF/F15-GP2-BR. The primer pair was designed based on the amino acid sequences GLGEPKF and FDLWEEI, respectively, which are conserved in the glucoamylase protein of Laccaria bicolor. This produce fragments of approximately 400 bp. Next, to amplify the whole genomic clone of PnGlu1, oligonucleotide primer PnGP2F/ PnGP2R were designed based on the nucleotide sequence of DNA fragments amplified by cassette PCR method. The produced fragment has significant homology with glucoamylase of L. bicolor. To investigate the relationship between different composition of medium and glucoamylase expression, we checked the expression level of glucoamylase gene by realtime RT-PCR and measurement of glucoamylase enzyme activity.

While Shandong Province is the largest historical and cultural and the first agricultural province in China, in recent years, due to the accelerated urbanization and modernization process, the conflict between traditional culture and modern civilization has had a serious impact on rural culture, and the ancient architecture among the villages in Shandong has also caused certain damage. These ancient buildings scattered in various villages in Shandong are important historical and cultural resources, the embodiment of Shandong traditional culture, and have an important role in the revitalization of rural culture and the enhancement of rural cohesion. However, research on the subsequent survey, conservation and restoration, and tourism development of ancient buildings in Shandong has lagged behind, and the role of these buildings in the inheritance of traditional Shandong traditional culture has not been given due attention, and their role in the construction of rural culture in the new era of Shandong has been seriously neglected. Tourism utilization is generally considered to be the preferred way to solve the conservation problems of ancient buildings. This study argues that the cultural revitalization of rural culture can be driven by the tourism and cultural industry of ancient buildings, and it can also provide a strong and sustainable power source for the rural revitalization strategy of the whole Shandong Province.