MAC-T cells, bovine mammary epithelial cell line, have been utilized to investigate bovine lactation system. A lactogenic phenotype of the cell is generally induced by combination of dexamethasone, insulin and prolactin (PRL). Effect of vitamin A derivative retinoic acid (RA), well reported as an inducer for differentiation in many cells, to MAC-T cell has not been studied. The objective of this study was to confirm effect of differentiation potential by RA treatment in MAC-T cells and to test effect of combination of RA and PRL treatment. In RA or PRL treatment groups, both has induced morphological change to secrete milk of MAC-T cells. Combination of RA and PRL treatment group has presented noticeable lactogenic phenotype among the all group. This phenotype observed at four days after treatment and showed critical morphological change that was rouphly spherical structure at eight days. RA alone treatment showed slightly inhibition of proliferation in the MAC-T cells, but co-treatment with PRL was improved the cell growth more than control group. MTT assay result and Bcl-xL/Bax ratio of mRNA abundance also was entirely consistent with earlier one. RA-induced differentiation of MAC-T cells has increased αs1-casein, αs2-casein and β-casein mRNA expression compared to PRL treatment group. Expression of αs1-casein, αs2-casein and β-casein genes represented the maximum value in the combination of RA and PRL treatment group at four days. The value of each casein gene expression was 4-, 5.5- and 5.9-fold, respectively, as compared with PRL alone treatment in the MAC-T cells. Protein level of β-casein releasing to the medium also induced the highest level at four days. These results provide evidence that RA can induce the differentiation of MAC-T cells and have synergetic effect with PRL.

본 연구는 사용 후 핵연료의 금속전환 공정에서 발생되는 폐용융염을 고형화하는 방법으로 실리카 함유 무기물을 이용하여 폐용융염을 열적, 수화학적 안정한 화합물로 전환하는 방법을 제안하였다. 실리카 함유 무기물(SAP)은 일반적인 sol-gel process로 합성되었으며, 및 로 구성된다. 제조된 SAP을 에서 폐용융염과 반응시켜 각 금속염화물에 대한 반응특성 및 열안정성을 조사하고, PCT 침출시험법을 이용하여 수화학적 안정성을 평가하였다. LiCl은 와 로, CsCl는 CS-aluminosilicate와 로, 는 로, 는 로 전환되었다. 9시간 동안 반응시킨 후, 금속염화물의 전환율은 였으며, 까지 열감량은 1wt%이하로 TGA(Thermo Gravimetric Analysis)로 확인하였다. Cs 및 Sr의 침출속도는 로 매우 높은 내침출특성을 나타내었다. 이상의 결과로부터, SAP으로 명명된 안정화제(stabilizer)는 금속염화물로 구성된 폐용융염에 대해 매우 효과적인 것으로 판단된다. SAP을 이용한 폐용융염의 고화처리방법은 후속적인 안정성의 검증과정을 통하여 폐용융염의 최종처분부피를 최소화할 수 있는 대안적인 고화방법으로 고려될 수 있을 것으로 기대 된다.