To understand the role of small heat shock protein (sHSPs) in rice plant response to various stresses such as the heat and oxidative stresses, a cDNA encoding a 24.1 kDa mitochondrial small HSP (Oshsp24.1) was isolated from rice by rapid amplification of cDNA ends (RACE) PCR. The deduced amino acid sequence shows very high similarity with other plant small HSPs. DNA gel blot analysis suggests that the rice genome contains more than one copy of Oshsp24.1. High level of expression of Oshsp24.1 transcript was observed in rice seedlings in response to heat, methyl viologen, hydrogen peroxide, ozone, salt and heavy metal stresses. Recombinant OsHSP24.1 protein was produced in E. coli cells for biochemical assay. The protein formed oligomeric complex when incubated with Sulfo-EGS (ethylene glycol bis (succinimidyl succinate)). Our results shows that Oshsp24.1 has an important role in abiotic stress response and have potential for developing stress-tolerant plants.

Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) is an upstream signaling molecule that has been shown to induce stress tolerance in plants. In this study, the AtNDPK2 gene, under the control of a stress-inducible SWPA2 promoter, was introduced into the genome of tall fescue (Festuca arundinacea Schreb.) plants. The induction of the transgene expression mediated by methyl viologen (MV) and NaCl treatments were confirmed by RT-PCR and northern blot analysis, respectively. Under salt stress treatment, the transgenic tall fescue plants (SN) exhibited lower level of H2O2 and lipid peroxidation accumulations than the non-transgenic (NT) plants. The transgenic tall fescue plants also showed higher level of NDPK enzyme activity compared to NT plants. The SN plants were survived at 300 mM NaCl treatment, whereas the NT plants were severely affected. These results indicate that stress-inducible overexpression of AtNDPK2 might efficiently confer the salt stress tolerance in tall fescue plants.

포장에서 생육된 잔디 포복경을 이용한 Agrobacterium 형질전환에 있어서 큰 제한인자였던 곰팡이 오염을 제거할목적으로 포복경 조직에 대한 새로운 살균법을 개발하고자하였다. 여러 가지 살균방법 중에서 30% NaOCl로 15분간처리한 다음 0.1% HgCl2로 25분간 처리 했을 때 포복경절편체의 생존율이 높았으며, 0.1% HgCl2로 처리시 800mbar의 진공처리를 5분간 실시했을 때 가장 효과적이었다.또한 Agrobacterium과 공동배양 시 2.5 mg/l의 amphotericinB를 첨가해 준 배지에서 배양했을 때 가장 높은 생존율을나타내었다. AmB의 처리는 Agrobacterium의 생장에 영향을 미치지 않았다. 또한 살균된 포복경으로 부터 신초의재분화에도 영향을 미치지 않았으며 곰팡이 오염만을 효율적으로 억제하는 것으로 나타났다. 이러한 결과는 포장에서 대량으로 생육시킨 잔디 포복경을 이용한 Agrobacterium형질전환 시 그 효율을 증가시키는데 큰 기여를 할 것으로추측된다.

With the aim to analyze stability performance of six promising barley genotypes, eleven yield related characters were evaluated employing varied irrigation treatments under the tropical climate of Northern part in Bangladesh. Analysis of variance(ANOVA), phenotypic index, regression co-efficient(bi) and deviation from regression(s2d ) of the individual genotypes were estimated to evaluate the stable performance of the genotypes. A significant interaction was observed between the genotypes and irrigation period(G~timesT ). Among all the genotypes, BSH-2 showed stable performance for plant height under different irrigation period, where P>~barX,~;bi~1~;and~;s2d~0. High phenotypic index, lower bi value and low deviations from regression were observed in case of spikelet number per spike and grain number per spike for genotype BSH-2 and plant height, spike length and harvest index per plant for BB-2 which suggest that those parameters were not usually affected by irrigation. On the other hand the genotype BSH-2 for tiller number and BB-1 for the fertile tiller number were not suitable for favorable moisture content, where P<~barX,~;bi>1.0~;and~;low~;s2d. Thus we suggest that genotype BSH-2 might have transmit high mean and increased phenotypic stability to the next progenies, which may consider as an ideal genotype for developing improved barely cultivars.