Sediment cell is renewable energy which produces electric energy using immanent ingredients or reducing power of marine sediment as natural resources. Also the cell has an advantage that environmental pollution can be reduced through conversion of organic and inorganic contaminants into inert matter with generation of the energy. In this paper, we compared characteristics of electricity generation of the two different sediment cells, and investigated the regeneration effect of the sediment cells with manipulation of the sediment such as mixing and re-positioning. The results showed that 14.1 W/m2 of power was obtained with the aluminum electrode, and the mixing of the sediment could increase the power by 4 W/m2 compared to the control. Also, mixing the sediment has kept electricity for 4 weeks at a relatively constant level, which implied ‘fuel regeneration effect'. Meanwhile, the sediment cell was proved to be effective in reduction of COD, which was up to 28.6%.