‘Samkang’ a new variety of oyster mushroom, fitting for the bottle culture, was bred and by mating between monokaryons isolated from MT05433 and AE1-1. In the major characteristics of fruit body, the pilei were thick and dark-gray and the pileistripes were thick and long with softness. It was great in elasticity and cohesivness of tissue as compare to chunchu-1ho. The optimum temperature for the mycelial growth was around 26~28℃ and that for the pinheading and growth of fruitbody was around 14~16℃. In the bottle culture, it was required around 26 days in incubation period and 4 days in primordia formation. The fruit body was grew vital and uniform. The yield were shown by 136.5g/bottle.