최근 농촌 인구 감소와 고령화로 노동력 부족 현상이 심화 되면서 농민들이 인력을 확보하는 데 어려움을 겪고 있다. 기 존의 노지 벼 육묘는 많은 공간과 노동력을 요구하며, 어린 묘 의 품질 관리가 어려운 문제가 있다. 본 연구에서는 컨테이너 형 수직농장을 활용하여 벼 육묘일수를 줄이고, 광 및 양액의 처리에 따른 생육 효과를 확인하였다. ‘고시히카리’와 ‘참드 림’ 두 품종의 벼를 지하수와 희석된 양액을 사용하여 각각 160와 355μmol·m-2·s-1의 광도에서 10일간 컨테이너형 수직 농장에서 재배하였다. 벼 육묘 결과, 모든 품종에서 10일 만에 이앙 가능한 초장을 확보할 수 있었으며, 양액을 처리하지 않 는 저광도에서 경제적 운영이 가능함을 확인하였다. 뿐만 아 니라 육묘된 벼는 순화과정에 문제가 없고, 본답 정식 이후에 도 기존 노지육묘와 생산성이 유사한 것을 확인하였다. 경제 성 분석을 통해 육묘판 생산 시 컨테이너형 수직농장 2동 이상 있을 경우 기존 노지육묘보다 비용 절감이 있음을 확인하였 다. 따라서 컨테이너형 수직농장을 활용한 벼 육묘는 비용 절 감과 노동력 부족 문제 해결에 기여할 수 있는 효과적인 대안 이 될 것으로 기대된다.

Phytophthora capsici an Oomycete pathogen is a major challenge to the pepper (Capsicum spp.) production around the world. Control measures are proved ineffective, so breeding resistant cultivars are the most promising strategy against the pathogen. Resistance against P. capsici is governed by quantitative trait loci (QTL). According to previous studies on QTL detection, the QTL on pepper chromosome 5 is a major contributor to resistance. In this study, to exploit the involvement of this QTL and identify its contributing genes, the F2 population derived from a cross between ECW30R and CM334 was inoculated with a medium virulence P. capsici strain JHAI1-7 zoospores at the 6-8 leaf stage. Composite interval mapping revealed two major QTLs; QTL5-1 from 7 days post inoculation (dpi) and QTL5-2 from 16 dpi on chromosome 5. To characterize and detect interactions of the two QTLs, near isogenic lines (NIL) were constructed by crossing Tean and recombinant inbred line (RIL) derived from a cross between YCM334 and Tean. RILs were screened with P. capsici strain MY-1 and resistant lines were selected. Among the resistance RILs most closely related to Tean were selected using AFLP and SSR genotyping data. These RILs were named as YT39-2 and YT143-2. To develop more advanced NILs, two rounds of marker-assisted backcrossing were done using a high-throughput SNP genotyping system (EPI Fluidigm, USA). Among the NILs derived from YT39-2, YT39-2-64 contains only QTL5-1 whereas YT39-2-61 and YT39-2-69 were identified to have both QTLs. On the other hand, YT143-2-55-7 with the highest Tean genetic background contains QTL5-1 only. In the next step, the 3 different NILs having QTL5-1, QTL5-2 individually and both QTLs will be identified. Furthermore, phenotyping and fine mapping will be done for the analysis of individual and interaction effects of QTLs.

Pepper mottle virus (PepMoV) is frequently occurring virus in pepper field. PepMoV infected plants show symptoms including mosaic leaf, distortion of foliage and fruit deformation. The dominant gene Pvr7 from Capsicum annuum ‘9093’ confers resistance to PepMoV. Previous research reveals that Pvr7 is located in 10 chromosome and linked to the dominant potyvirus resistance gene Pvr4 and Tomato spotted wilt virus (TSWV) resistance gene Tsw. To identify the Pvr7 gene, we constructed an intraspecific F2 mapping population from a cross between C. annuum ‘9093’ (PepMov resistant) and C. annuum ‘Jejujaerae’ (PepMoV susceptible). Resistance of F2 plants were screened with green flouorescent protein (GFP) tagged PepMoV. Genomic DNA was extracted from F2 individuals and markers were developed using C.annuum ‘CM334’ whole genome sequence (WGS). Several single nucleotide polymorphism (SNP) markers that were co-segregated with Pvr7 were developed. We are expecting that this Pvr7 SNP marker can be used to breeding PepMoV resistant cultivars and fine mapping of Pvr7.