This experiment was conducted to prevent the contamination of parasite egg on vegetables for supporting safety production of leafy vegetables. After the chinese cabbages and soil samples were collected at 5 cities of Gyeonggido during 2007-2008, the existence of parasite egg was surveyed. All collected samples had no parasite egg, so the chinese cabbages produced in Gyeonggi area were assumed parasite egg-free. To examine the characteristics of parasite eggs, the pig roundworms, Ascaris suum, were collected from the intestine of infected pig and the parasite eggs were collected from the uterus of A. suum. The eggs of A. suum developed to embryonated eggs, which can infect humans, in 20℃-30℃, but not 15℃ and 35℃, when cultured at different temperatures. The eggs developed to embryonated eggs after drying for 0-24h when cultured after different drying times. Effect of soakage different salt solution and times showed that the eggs developed to embryonated eggs after soakage at 0-25% salt solution for 0-24h. For eliminating the parasite eggs attached leafy vegetables, it was efficient to soak at salt solution for 5 minutes and washing 5 times with water.

Western flower thrips, Frankliniella occidentalis(Perg.), is one of the major pests of lettuce, Lactuca sativa. To develop the economic thresholds for western flower thrips on lettuce, the thrips was inoculated into two types of lettuce, green deeply lobed leaf lettuce which was harvested every week and red frilled & crinkled leaf lettuce which was harvested one time, with a density of 0, 1, 3, 7, 10 thrips per one lettuce after planting 1 week and 3 weeks at the plastic house in 2006, and 0, 1, 3, 5, 7 thrips per one lettuce after planting 1 week in 2007. There was close correlation between the initial thrips density and the marketable quantity of lettuce : y = -155.36x + 3564.7, r2=0.9724('06), y = -167.82x + 3474.5, r2=0.8897('07) in the green deeply lobed leaf lettuce inoculated the thrips after planting 1 week, y = -109.65x + 3549.1, r2=0.9384('06) in the green deeply lobed leaf lettuce inoculated the thrips after planting 3 weeks, y = -197.09x + 3334.9, r2=0.9707('06), y = -244.08x + 3425.9, r2=0.8914('07) in the red frilled & crinkled leaf lettuce inoculated the thrips after planting 1 week, y = -101.07x + 3457.6, r2=0.7334('06) in the red frilled & crinkled leaf lettuce inoculated the thrips after planting 3 weeks. The economic threshold for western flower thrips on lettuce was ranged 0.20～0.30 thrips per one lettuce in the blue type lettuce and 0.14～0.33 thrips per one lettuce in the red type lettuce.

In this study, a field study was conducted to investigate the relationship between high-resolution remote images and the volumetric moisture, and the number of compaction. Changes in the shape of the surface and soil moisture content were observed and correlated with the number of compactions using roller equipment. As the compaction is repeated, the surface is flattened and the terrain curvature decreases and converges to zero. In particular, the tangential curvature changes as the number of compactions increase. Due to soil compaction, the vegetation index changed from a positive to a negative value, and most of the test site area was homogenized with a negative index. This suggests a decrease in porosity and an increase in volumetric water content associated with increasing soil compaction. Soil moisture, measured using a frequency domain reflectometry(FDR) sensor, tends to increase proportionately with the number of vibration compactions, but the correlation between the number of compactions and soil moisture is unclear. This study suggests that while it is necessary to consider the reproducibility of the experiments performed, the compaction quality of the soil can be evaluated using high-resolution terrain factors and soil moisture.

본 연구에서는 상백피의 소화효소 저해활성과 3T3-L1 전지방세포의 분화 억제능을 기반으로 항비만 효능소재로 서의 활용가능성을 평가하였다. 상백피의 에탄올 추출물 (MRE)은 α-amylase와 α-glucosidase, pancreatic lipase를 활 용한 소화효소 저해활성 평가 실험에서 각각 7.86±0.36, 0.12±0.03, 7.93±0.11 mg/mL 의 IC50 값을 보이며 우수한 억제 활성을 나타냈다. 또한 3T3-L1 전지방세포를 활용한 세포분화억제효능실험에서 MRE 처리군의 세포내 지방 축 적율은 농도 의존적으로 감소되었다. 상백피의 항비만 작 용 기전을 구명하기 위하여 adipogenesis 및 lipogenesis와 관련된 유전자 발현양상을 분석한 결과, 상백피 추출물 처 리군에서는 생체내 지방대사 조절에 중요한 역할을 하는 FAS와 ACC 뿐 아니라 adipogenesis와 lipogenesis와 관련된 주요 전사요소인 PPARγ와 C/EBPα, SREBP-1c의 유전자 발현이 현저하게 억제되었다. qRT-PCR 분석 결과, 상백피 추출물의 anti-adipogenesis 효능은 전사단계에서의 관련 유 전자 발현억제에 기인한다고 판단되었다. 본 실험결과 상 백피 추출물은 전지방세포의 분화와 세포내 지질합성을 저해하고 비만과 관련 된 소화효소에 대한 저해활성을 나타 내었다. 이러한 결과를 기반으로 상백피의 비만 예방 소재 로서의 잠재적인 가능성을 확인하였다.