This study was carried out to confirm the effects of luteotrophin, human chorionic gonadotrophin (hCG), and an anti-luteolytic agent, flunixin meglumin (FM), on pregnancy rates in Hanwoo with in vitro produced (IVP) embryo transfers (ET), and to research the effects on the estrus cycle. Treatments included hCG and FM administration 3~10 minutes prior to ET. Also, pregnancy rates were compared with lidocane treatment and FM treatment prior to ET. The results are shown below. 30-day pregnancy rate was 76.7% in the hCG-treated group and 75.7% in the FM-treated group. Both rates were higher than the 70% rate for the control group. 42-day pregnancy rate was 76.7% in the FM-treated group. This was higher than 66.7% recorded for both the hCG-treated and control groups. The pregnancy rate of the hCG-treated group was high at Day 30 (76.7%) but low at Day 40 (66.7%), and there were no differences from the FM-treated and control groups. The recurrent estrus rate of infertile individuals at 2 weeks after ET was 36.4% in the hCG-treated group, under 71.4% in the FM-treated group and 80.0% in the control group. The non-pregnancy rate of individuals without recurrent estrus was 18.2% in the hCG-treated group, which was higher than the 0% rate in both the FM-treated and control groups. The pregnancy rates were higher in the FM-treated group than the Lidocane-treated group with 72.3% versus 67.5% in the heifers and 48.9% versus 43.6% in the cows. From the above results, the FM treatment proved more effective than the hCG treatment and no treatment whatsoever in increasing pregnancy rates after ET. In addition, hCG treatment was shown to be undesirable due to the deviations it caused in the reproductive physiology of the hCG-treated recipients. Therefore, in our study, the FM treatment resulted in a higher pregnancy rate than either lidocaine treatment or no-treatment in the trials of ET.

As a simple and economical method for in vitro produced embryos, we have used BSA instead of serum for the production and embryo transfer of Hanwoo in vitro fertilized (IVF) embryos and obtained the following results: 1) When using serum (FBS; fetal bovine serum) or BSA-containing culture media as the initial culture media for immature oocytes, it is regarded as inappropriate to add only BSA to the culture solutions from maturation of the immature oocytes to development stage culture, but serum still needs be added though there is no significant difference in the concentration, with a change from 5% to 10%. 2) The results of culturing IVF embryos after development (4 cell stage) in the Medium199 solutions containing BSA instead of serum (FBS) showed that 0.3% BSA concentration is not optimal and 0.5% or higher BSA concentration has no significant difference among 0.5%, 0.7%, 1% and 2% (p > 0.05). 3) The post-freezing survival ratio after development in 5% FBS-Medium199 showed that 1% BSA concentration of the culture solution is the most suitable in the BSA concentrations of 0.3% (51%), 0.5% (67%), 0.7% (69%), 1% (77%) and 2% (75%). 4) The pregnancy rates of the transplanted fresh(not frozen) blastocyst had no significant concentration dependency (p > 0.5), and the average pregnancy rate was 63.8%. 14% of overweight calves were found among the calves given birth to by the transfer of IVF blastocysts cultured in the serum-added culture solution, but none was found in the experimental groups in which BSA was added instead of serum.

초 록 본 실험은 Corynebacterium속 식물병원세균에 관한 연구의 일환으로서 Corynebacterium속 식물병원세균의 종 및 속으로서의 특성을 구명하기 위하여 행하여진 것으로서 균체구성단백의 정량은 Lang의 Messier 변법을 사용하였으며 균채 구성단백의 아미노산정량은 미생물 정량에 의하였다. Corynebacterium 속 식물병원세균은 타속의 병원세균에 비하여 다소 적은양의 균체 구성단백을 가지고 있었으며 균체구성단백의 아미노산, Cystein, Trypthophane, Histidine, Phenylalanine 및 Isoleucin에 있어서도 같은 결과를 나타내었다. 허나 이 현상은 종적 혹은 개체의 균주의 특성으로서 분류에는 관계를 갖지 않고 있음이 밝혀졌다

본 실험은 Corynebacterium속 식물병원세균에 관한 연구의 일환으로서 Corynebacterium속 식물병원세균의 종 및 속으로서의 특성을 구명함과 동시에 C. sepedonicum이 생성하는 Rumiflabin에 기초를 둔 ultraviolet method의 재검토를 위하여 행하여 진 것으로 vitamin B군의 정량은 미생물 정량에 의하였다. 대다수의 식물병원세균은 Thiamine (vitamin ), Nicotinic acid, Biotin 및 안식향산을 생장물질로서 사용하였다. Riboflavin은 corynebacterium속 식물 병원세균 (C. rathay 및 C. fascians는 제외)은 물론 타속의 식문병원세균도 생성하는 점으로 Corynebacterium속 식물병원 세균의 특성이 아니라는 점이 구명되었으며 sepedonicum에 의하여 발생되는 감자의 윤부병과 청고병과의 감별에 사용되어온 Ultra-violet light법은 재검토되어야 한다.