This study aimed to recover the ovarian function through in vitro culture of preantral follicles from aged mice. First, we isolated the preantral follicles from ovaries of sixty-seven-week old B6D2F1 mice with decreased fecundity to know how many follicles were present in them, which was 6 preantral follicles including 2 primary, 2 early secondary and late secondary follicles from 8 aged mice. It was confirmed that a few follicles (~2) were present in aged mice through histological analysis compared to adult mice as control. The 9 days of in vitro culture of preantal follicles showed in vitro growth and induced maturation after treatment with hCG (2.5 IU/mL) and EGF (5 ng/mL). Cumulus cells in the cumulus-oocyte complexes (COCs) were removed using hyaluronidase and oocytes at the germinal vesicle (GV) and GV breakdown (GVBD) were obtained from preantral follicle culture of aged mice in vitro. In conclusion, these observations demonstrated that there still were a few preantral follicles in the ovaries of 67 week-old mice, which we were able to culture in vitro and oocytes were obtained from them. This study proposed an in vitro culture system using preantral follicle as a therapeutic strategy for fertility preservation in humans for assisted reproductive medicine.

본 논문에서는 응답성이 우수한 반도체 스위칭 소자들을 이용하여 선로전압을 항상 피드백 받고 있다가, 아크나 스파크가 발생하면 전압파형의 순간적인 감쇠 또는 전압 왜형을 감지하여 전기를 차단하는 고속 고정밀의 전압센싱형 아크차단기를 개발하고자 한다. 또한 제안한 아크차단기는 전기사고 발생 시 상전압과 접지간의 강제적인 단락회로를 형성하여, 기존에 설치된 RCD를 누전 또는 과전류로 인식시켜 신속히 RCD를 차단시키는 신개념의 제어기법을 채택하였다.