본 연구는 농업유전자원센터에 보존되어있는 우리나라 및 불가리아 재래종 강낭콩 유전자원 130점을 대상으로 형태적특성을 조사하고, 155점에 대해 분자마커를 이용하여 우리나라와 불가리아 재래종 강낭콩 유전자원의 유전적 다양성을 분석하였으며 결과는 다음과 같다. 1. 개화일수는 최소 50일에서 최대 79일, 평균 54.5일이었으며 성숙일수는 최소 25일, 최대 64일, 평균 38.9일 이었고 생육일수는 최소 83일, 최대 123일, 평균 93.4일이었다. 불가리아 재래종 자원들이 한국 재래종 자원보다 개화일수는 3.1일, 성숙일수는 3.6일, 생육일수는 6.7일 늦은 것으로 조사되었다. 2. 공시자원의 100립중은 19~71g 사이에 분포하였고, 평균 100립중은 41.2 g이었으며, 우리나라 재래종 자원은 평균 100립중이 40.9 g, 불가리아 재래종 자원은 42.4 g 이었다. 3. 조사대상 자원의 질적형질 중 생육습성은 한국 자원은 직립형이 87.6%인 반면 불가리아 자원은 덩굴성이 60.0%였으며, 한국 자원의 화색은 보라색 줄무늬가 있는 백색이 80%로 가장 많았으나 불가리아 자원은 백색자원이 52%를 차지하였다. 종피색은 한국 자원은 적색이 61%, 불가리아 자원은 백색이48%를 차지하여 국가간 선호도와 용도에 따른 차이가 있을것으로 보였다. 4. 10개의 SSR 마커를 이용하여 다양성을 분석한 결과 PIC 0.6181을 얻었으며, 국가별 분석 결과 다양성은 gene diversity와 PIC로 볼 때 불가리아 자원이 다양성이 높은 것으로 조사되어 불가리아에서 도입한 강낭콩 유전자원이 강낭콩 수집단의 유전적 다양성 증대에 기여한 것으로 평가된다.

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. ‘Milky way’. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 μl PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

The objective of this study was to analyze the genetic diversity using SSR marker and investigate the fatty acid composition of perilla (P. frutescens var. frutescens) germplasm. Genetic diversity among 95 accessions, which consisted of 29 weedy types and 66 landrace accessions, was evaluated based on 12 SSR markers carrying 91 alleles. The mean values of observed (HO) and expected heterozygosities (HE) were 0.574 and 0.640, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into two distinct groups, which were the landrace, moderate and weedy type, genetic distance (GD) value was 0.609. The physicochemical traits about crude oil contents and fatty acid compositions were analyzed using GC. Among tested germplasm, the total average oil contents (%) showed a range from 28.57 to 49.67 %. Five fatty acids and their contents in the crude oils are as follows: α-linolenic acid (41.12%-51.81%), linoleic acid (15.38%-16.43%), oleic acid (18.93%-27.28%), stearic acid (2.56%-4.01%), and palmitic acid (7.38%-10.77%). The average oil content of wild types was lower than landrace, and the oil content of middle genotype accessions was higher than other germplasm, but no significant variation between landrace and wild types was shown. Nevertheless, IT117174, landrace of Korea, was highest in crude oil content (47.11%) and linolenic acid composition (64.58%) among the used germplasm. These traits of the selected accessions will be helped for new functional plant breeding in perilla crop.

Buckwheat (Fagopyrum esculentum Moench), one of the minor crops grown in Korea belonging to the Polygonaceae family, is an annual crop widely cultivated in Asia, Europe, and America and has a character of outcrossing and self-incompatibility. The objective of this study was to analyze the genetic variability, phylogenetic relationships and population structure of buckwheat landraces of Korea using SSR markers. Ten microsatellite markers have been detected from a total of 79 alleles among the 179 buckwheat accessions were collected from Korea. The number of allele per marker locus (NA) ranged from 2 (GB-FE-001, GB-FE-043 and GB-FE-055) to 31 (GB-FE-035) with an average of 7.9 alleles. GB-FE-035 was the most polymorphic with the highest PIC value 0.93. Major allele frequencies (MAF) for the 10 polymorphic loci varied from 0.12 to 0.97 with a mean allele frequency of 0.57. The expected heterozygosity (HE) values ranged from 0.05 to 0.94 with an average of 0.53. The observed heterozygosity (HO) ranged from 0.06 to 0.92 with an average of 0.42. The overall polymorphic information contents (PIC) values ranged from 0.05 to 0.93 with an average of 0.48. The landrace accessions of buckwheat used in the present study were not distinctly grouped according to geographic distribution. The study concludes that the results revealed genetic differentiation was low according to the geographic region because of outcrossing and self-incompatibility. We reported that our analyses on the genetic diversity of common buckwheat cultivars of Korea were performed by using of microsatellite markers.