1) 쇠파리의 누대 실내사육을 위한 온도는 약 가 좋으며, 이때 유충기간은 약 6.8일, 용기간은 5.3일, 산란전기간은 10.4일, 성충의 수명은 약 30일이었다. 2) 인공사육에 있어서 용화율은 우화율은 였으며 성비는 1 : 1이었다. 3) 용의 체중량은 약 14.5mg이었으며, Wheat bran medium 보다 Standard medium이 사육성적이 좋았다. 4) medium 125gr에 대한 난의 접종수는 약 310개가 가장 적합하였다. 5) rectangular cage를 사용할 경우, 성충의 resting place는 가 적합할 것으로 본다.

The present study examined the possibility of long term storage, by cryopreservation in liquid nitrogen, of the sperm of Filefish (Thamnaconus septentrionalis), and the changes in motility, survival rate and ultrastructure of the sperm after freezing and thawing. The sperm was collected by stripping and stored on ice until experiments. For selection of the immobilizing solution, diluted artificial seawater (ASW) of 20, 30 and 40% were tested. The sperm motility was significantly inhibited in 30% ASW, and restored entirely after 100% ASW was added again. Two cryoprotectants, dimethyl sulfoxide (SO) and glycerol, were added to 30% ASW to formulate the extenders at the concentrations between 5 to 20% by volume for freezing. The sperm was diluted at the ratio of 1 :6 with the extenders, inserted into 0.5ml plastic straws and frozen at a freezing rate of /min to after equilibration for 10 min at room temperature, followed by plunging into liquid nitrogen. The straws were thawed in a water bath for 15 sec. The highest post-thawed sperm motility and survival rate were obtained with 5% glycerol Afterward, the effect of different freezing rates was examined using 5% glycerol as a cryoprotectant, and the rate of /min to showed the best result Some ultrastructural changes of sperm, such as the detachment of plasmatic and nuclear membranes, destruction of mitochondria, were observed after cryopreservation. Morphological normality of the sperm in 5% glycerol frozen at the ratio of 1/min to was better than that of others.

The present study examined the possibility of cryopreservation of the D-shaped and umbo larvae of arkshell (Scapharca broughtonii), in terms of the survival rates after freezing and thawing. D-shaped and umbo larvae of arkshells were obtained from a shellfish farming on Yosu city. The average shell lengths were m and , respectively. Five cryoprotectants (CPAs), dimethyl sulfoxide (DMSO), glycerol, ethylene glycol (EG), propylene glycol (PG), and methanol, were tested at the concentrations of 1.5, 2.0 and 2.5 M. After larvae suspended in CPAs, cryoprotectants were loaded in 0.5 ml straws at a larval density of 50-100 larvae per straw, and epuilibrated for 10 and 20 minute at room temperature (), repectively. Straws were cooled at a rate of /min from to , held for 5 min at , and then cooled at /min to and equilibrated for 5 min followed by plunging in liquid nitrogen. After storage in liquid nitrogen for 1 day, straws were thawed in a water. As soon as straws were observed to melt, larvae were diluted with an equal volume of ASW and then washed twice with a large volume of ASW at an interval of 2 min to unload the CPAs. The results showed that after equilibration for 10 and 20 minute at room temperature, no larvae survived using methanol as CPAs, and it was observed that larval shells all open slightly, and larval flesh broke down and slopped over the shells. The highest survival rates (D-shaped larvae: 77.6%, umbo larvae: 59.3%) were obtained with 2M DMSO, and 1.5M glycerol yielded survival rates of 53.8% for D-shaped larvae and 37.5% for umbo larvae. The surviving D-shaped larvae showed active rotary motion and perfect membrane integrity and cytoplasmic normality, and the vigorous movement of veliger cilia was observed inside the closed shells. The breakdown of tissue occurred in the abnormal larvae, and the isolated cell often run out of shells.

호밀 적ㆍ만기 파종에서 종자성숙 및 파종량이 출아, 생육, 청예 건물수량 및 종실수량에 미치는 영향을 구명하고저 경북 경산군 영남대학교 농장에서 재래종(팔당호밀)을 공시하여 파종기 10월 28 일(적기) 및 11월 11일(만기)로 하고 파종기별로 출수후 45일 및 30일에 수확한 종자를 파종량을 달리하여 ( 1m2 당 300입, 450입, 600입, 750입, 900입) 시험을 실시하였다. 출아수 및 분얼수는 적기파종에서 출수후 30일과 45일 종자간에 차이가 없었고, 파종량을 증가할 때 출아수가 증가하고 분얼수는 감소하는 경향이 있었고, 만기파종에서는 출수후 45일 종자에서 30일 종자보다 출아수와 분얼수가 많았으며 파종량 증가에 따른 차이는 인정되지 않았다. 만파에서 출수후 30일 종자는 파종량 증가로서 적정주수 확보가 어렵다. 출수기는 적ㆍ만파에서 출수후 30일 종자가 2~3 일 늦었다. 청예수양은 만파에서 크게 감소하였고 적ㆍ만파모두 파종량을 높일 때 청예수량도 증대되어 적기파종에서 750립 / m2 ( 15kg/ 10a)의 파종량에서 최대가 되었고, 출수후 30일 종자는 만파에서 현저하게 낮았다. 수수 및 1000입중은 적기파종에서 만기파종보다, 그리고 만기파종에서는 출수후 45일 종자로서 파종량 750립/m2 ( 15kg/10a) 이상에서는 적기파종의 종실수량 수준까지 접근할 수 있었다. 출수후 30일 종자는 만기파종에서 파종량을 증가하여도 출아율이 낮고 청예 및 종실수량이 심하게 감소되어 종자로 이용하기는 불안정하다.