본 연구의 목적은 스포츠 팀구축프로그램 개발 시 요구사항을 분석하고, 팀구축프로그램의 모형과 활동을 구성하여 팀구축프로그램을 개발하는데 있다. 스포츠 팀구축프로그램 개발을 위하여 요구조사 분석단계에서 중고등학교 여자축구선수 64명의 청소년 여자축구선수와 프로그램 개발의 타당성과 신뢰성 제고를 위한 전문가 회의를 위해 축구전문가 3인과 스포츠심리학자 3인으로 구성된 전문가 6인이 참여하여, 총 70명을 연구참여자로 선정하였다. 연구 절차는 팀구축프로그램 요구조사 및 분석과 프로그램 모형 개발 및 내용 구성의 2단계로 구성하였다. 이상의 연구절차를 통하여 본 연구에서 얻어진 결과는 다음과 같다. 첫째, 팀구축프로그램에 요구되는 요인 탐색 결과 팀구축 강화요인은 의사소통, 팀내 대인관계, 팀목표로, 팀구축 저해요인은 팀내 대인관계와 의사소통으로 나타났다. 둘째, 요구분석 결과에 기초하여 팀구축프로그램의 목표는 팀내 대인관계와 의사소통으로 설정하였다. 셋째, 팀구축프로그램의 모형 및 내용구성은 팀구축 목표설정, 대인관계 변화, 의사소통 변화의 3단계 10주 활동으로 구성되었다. 본 연구에서 개발된 스포츠 팀구축프로그램은 향후 팀구축프로그램 관련 연구의 기초 정보를 제공하고 스포츠 현장에서 스포츠심리학자와 지도자에 의해 활용될 것이라고 생각된다.
We compared the long-term metabolic effects of equal amounts of carbohydrate from potato, rice and buckwheat on glycemic indices and blood lipids in healthy subjects. Nine healthy volunteers-2 men and 7 women were studied. All subjects ate diets based on the same-7-day rotating menu differing only in that the major source of carbohydrate (about 50% of daily total calories) came either from buckwheat, rice or potato. The study was conducted with a triple crossover design over three 7 day periods. On the morning of the 8th day, fasting blood was drawn from each subject to determine serum glucose, insulin, triglycerides, total and HDL-cholesterol. Subjects were then asked to eat breakfast with their respective carbohydrate within a 20 min period. Blood samples were drawn at 30, 60, 120 and 180 min after the start of breakfast to determine glucose and insulin levels. At 30 min the glucose response to the rice meal(7.15mmol/L) and potato meal(6.71mmol/L) were greater than the response to the buckwheat meal(5.855mmol/L) (P < 0.05). The mean area under the glucose response to the curve following the rice meal was greater than that following the buckwheat meal(P < 0.05). The insulin responses to the potato and rice meals at 30 and 60 min were greater than those to the buckwheat meal (P < 0.05). The mean area under the serum insulin response curve after the rice meal was greater than of buckwheat. Blood lipids, uric acid and glycosylated hemoglobin were not affected by the three meals. The study shows that the buckwheat meal has more beneficial effects on glycemic indices than either the rice meal or potato meal in healthy subjects.
Transgenic Petunia hybrida cv. Rosanpion was produced by Agrobactepium tumefaciens LBA4404 harboring a binary vector pBI 121 containing β-glucuronidase (gus) and neomycin phosphotransferase (nptII). For genetic transformation, leaf discs were precultured on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA (MNB) for 2 days and cocultured for 15 mins with A. tumefaciens. For selection of transformant, leaf discs were transferred to fresh MNB containing 50 mg/L kanamycin and 500 mg/L cefotaxime. Eighteen plants were regenerated and four were confirmed by PCR for detection of gus and nptII gene integrated into the nuclear genome of petunia ‘Rosanpion’. Using this transformation system, we expect that transgenic petunia ‘Rosanpion’ incorporating a useful gene can be produced.