Many higher fungi were collected at Mt.Sunun, Mt.Kirin, Samre-up, Whaam-sa and Mt.Unjang from June 1999 to June 2000. They were identified. As the resulting, Pluteus thomosonii, Mycena polyadelpha, Mycena latifolia, Omphalina barbularum, Hygrocybe trunda var.macrospora, Cortinarius iodes and C. glaucopus are newly to Korea. They were designed Korean common name by author.

Growth and yield of licorice were investigated under the different conditions of ridge height, planting density, and irrigation in order to establish its cultural practices for the domestic production with the aim to substitute the import. Seedlings were grown under low ridge(20cm) and high ridge(40cm) in low density plot(60×30cm) and high density plot(40×30cm), respectively. The low ridge cultivation of large seedlings increased plant height and root length under low density, and stem and root diameter under high density compared to the high ridge cultivation. In the high ridge cultivation, high density plot was 1.1 to 1.3 times in plant height, root length, stem and root diameter as high as low density one. Fresh and dry weight of plant and root in high ridge were 1.3 to 1.5 times as high as those in low one. The growth of small seedlings(4~10g) were generally poor compared to that of large seedlings. High density plot in low ridge showed the good growth characteristics including plant height, root length, stem and root diameter, and number of branch. High density plot was 1.4 to 1.6 times in fresh and dry weight of plant and root as high as low density plot. In the seasonal changes of growth under various irrigation regimes, the twice irrigation a day produced the more number of leaf than the other regimes since around 46 days after transplanting. The former irrigation resulted in 1.2 to 1.4 times in plant height as long as the other irrigations around 26 days after transplanting and then the difference was increased to 1.6 to 2.0 times around 64 days after transplanting. Under the twice irrigation a day, plant height, root length, stem diameter, root diameter, number of leaf, fresh plant weight, dry plant weight, fresh root weight, dry root weight were 1.6 to 2.0, 1.1, 1.2 to 1.6, 1.3 to 1.8, 1.9 to 2.7, 1.7 to 8.0, 1.6 to 2.8,2.0 to 3.0, 1.6 to 2.7 times as high as those under the other irrigation regimes, respectively.

Growth and yield of licorice were investigated under the different conditions of photoperiod and shading in order to establish its cultural practice for the domestic production with the aim to substitute the import. The photoperiod was adjusted to 8,10, and 12 h by shielding plants from the light with blackout curtain. Large seedlings(11-20g) appeared to be affected by photoperiod since around 65 days. Most of growth parameters, including plant height, number of leaf, fresh and dry weight of plant and root, were the highest in 12 h photoperiod among all the photoperiod levels, excepting stem diameter which was the highest in 10 h photoperiod(4.5mm). Each photoperiod was similar to each other in root length and diameter. Small seedlings(4-l0g) showed a similar trend to large seedlings. The results from field photoperiod experiment demonstrated that 12 h photoperiod was also the best among three photoperiod treatments in plant height, stem diameter, number of leaf, root length, fresh and dry weight of plant and root. The effect of shading was tested under the three levels of control (0%), half-shading (55%), and full shading (90%). Shading remarkably suppressed the growth and yield, compared to no-shading. Although plant height and root length were little affected by the shading, stem and root diameters were heavily reduced.

To improve the iron content of red pepper, we have transferred the entire coding sequence of the ferritin gene(Fpl) into Capsicum annuum (L. cv. Chungyang and Bukang) by Agrobacterium mediated transformation. Transformants were found to contain the Fp1 gene at up to three loci, increased distinct iron content changes. In transgenic plants, iron content was as much as 7-fold to 8-folds greater than that of their untransformed counterparts. Furthermore, the Rl progenies from transformant(A7, A8) co-segregated into a 15:1 ratio for both Kanamycin resistance and genotype of high iron.

Major characteristics of new Waesungri maize inbred line has multi-tiller and ears: five to six tillers and seven to eight ears per plant and flowering date of Waesungri was delayed about 18 and 24 days compared to Mo17 U.S line and IK4 Korean local lines, respectively. Number of ears, fresh and dry weight per plant were significantly different among all tested hybrids including Waesungri/Sinkihong hybrid under different planting times and densities. Especially, both fresh and dry weight of IK1/FR140//Waesungri F1hybrid were significantly higher at high planting density. In kernel weight per unit area, Wnesungri/Sinkihong hybrid was high at high density and IK1/FR140//Waesungri hybrid was high at low planting density. As results of analysis of variance, flowering date was shown a significantly different both planting times and varieties, while other characters including stem height were shown very variable in interactions with enviromental factors.

Transgenic Petunia hybrida cv. Rosanpion was produced by Agrobactepium tumefaciens LBA4404 harboring a binary vector pBI 121 containing β-glucuronidase (gus) and neomycin phosphotransferase (nptII). For genetic transformation, leaf discs were precultured on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA (MNB) for 2 days and cocultured for 15 mins with A. tumefaciens. For selection of transformant, leaf discs were transferred to fresh MNB containing 50 mg/L kanamycin and 500 mg/L cefotaxime. Eighteen plants were regenerated and four were confirmed by PCR for detection of gus and nptII gene integrated into the nuclear genome of petunia ‘Rosanpion’. Using this transformation system, we expect that transgenic petunia ‘Rosanpion’ incorporating a useful gene can be produced.

This study was conducted to investigate the occurrence of Soil borne wheat mosaic virus(SbWMV) in barley fields in Korea and to examine the host pathogenicity of SbWMV. By using the ELISA test, SbWMV was detected in the six regions : Suwon, Milyang, Jinju, Youngkwang, Iksan, and Chonju. SbWMV was isolated from the two strains, Albori strain from Jinju and Eunpamil strain from Milyang. SbWMV was collected from leaves showing mosaic, yellowing and necrosis stripes. SbWMV was inoculated mechanically on 1∼1.5 leaf stages with leaf-rubbing to identify the host pathogenicity of 36 Korean barley cultivars, a wheat cultivar, two rye cultivars, three Japanese barley cultivars and Chenopodium amaranticola. Viral sympoms of inoculated leaves appeared on moulted loaves about 4 to 6 weeks of inoculation. Baegdong and Tapgolbori, infected from Albori strain and Eunpamil strain infected from Samdobori showed much higher susceptibility than C. amaranticola and C. quinoa which showed ring spots and chlorotic spots respectively. Virus particles were observed by the electron microscope. They were rod-shapes, which are bipartite, of 142 nm or 281 nm in length with 20 nm diameter on infected leaves. Specific detection and identification of SbWMV was set up using the RT-PCR. PCR fragments of SbWMV(0.5kb) were obtained by using the designed primers for SbWMV RNA 2.

Seven genetic lines of Bupleurum falcatum L. from different geographical regions were analysed for saikosaponin contents and chromosomal numbers. The somatic chromosome numbers of B. falcatum originated from Euisong, Iri, Milyang, Sangnam, Taejon, and Youngchon were 2n=20 while Mishimasaiko showed 2n=26. However, chromosome features were different in plants grown in different geographical regions. Generally, Korean lines had higher saikosaponin contents than Mishimasaiko which is Japanese and Sangnam lines had highest saikosaponin contents compared to other tested lines.

The effect of carbenicillin on the dedifferentiation and the regeneration efficiency of plant tissues of horseradish(Armoracia rusticana) was evaluated, Inhibition effect for callus initiation was observed when leaf blade, root and petiole segments were grown on MS medium containing 500 mg/L to 2000 mg/L carbenicillin and 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The regeneration of horseradish shoots from leaf blade, root and petiole explants were decreased as the addition of carbenicillin increased from 1000 mg/L to 2000 mg/L in MS medium containing 0.5 mg/L of 6-benzylaminopurine (BAP) or kinetin. Especially, 500 mg/L carbenicillin treatment significantly inhibited shoot induction when leaf blade explants were grown on hormone-free MS medium. It was suggested that the toxic effects of combinations of carbenicillin and 2,4-D may be due to high auxin activity levels.