Total iron content and ferritin distribution have been determined in red pepper(Capsicum annuum L.) during development stage under conditions of iron nutritional status from hydroponic culture. Color of the leaves become chlorotic on iron deficient and high concentration. The plant height on each iron concentration had retarding effect at concentration lower than 25μM and greater than 12525μM. In normal green leaves. Total iron content was almost constant with a mean value of 2.5μmole of iron/mg of dry matter, except at 63day, for which it increases slightly to 4μmole. Howere, iron content of chlorotic plants grew on iron free medium was not almost detectable. Also in post chlorotic leaves(++Fe), iron content was evidently increase unitl 7days after transfer on liquid medium, but decreased from after 14days. Also, ferritin protein analysed total protein extracts prepared from leaves of different ages using antibodies raised against ferritin protein. Ferritin protein deereased progressively during the first week of germination and was not detectable in vegetative tissues. Ferritin protein in post chlorotic leaves wasevidently strongly cnhanced until 11days after transfer on liquid medium but decreased until the leves became chlorotic.

A cDNA Fragment encoding iron storage protrin generated by polymerase chain reaction(PCR) using highly conserved regions of ferritin related genes were used to sereen a red pepper cDNA library. cDNA clone was designated as Fp1. Fp1 clone contatines a 5' nontranslated region of 51dp containing stop conds. Down stream from 5' UTP. an open reading frame of 750bp was observed. followed by a 3' UTR of 272bp. The deduces amino acid sequence of red pepper protein(Fp1) showed 84%, 48% and 36% identity with soybean(SolC). human(HuL H) and horse spleen(HoS-L) ferritin mRNA accumulation in response to iron. Ferritin mRNA accumulation was transient and particularly abundant in leaves. reaching a maxmum at 12h. The level of ferritin mRNA in roots was affected to a lesser extent than in leaves.

To establish direct multipropagation through organogenesis from nodal explants of Anoectochilus formosanus Hayata, the nodes were cultured on LS medium containing various concentrations of 6-benzyladenine(BA). High plant regeneration and adventitious bud formation were obtained from supplemented with 4.0mg/l of BA. Plant height was promoted by adding 0.3% activated charcoal. Plantlet regeneration capacity from nodes was depended on nodal parts on the stem, upper position was the best comparing with intermediate and lower.

This paper describes the variations in eight agronomic traits in three unadapted local landraces and an inbred cultivar of corn. To compare the agronomic traits in field evaluation with molecular marker evaluation the genotypes of the plant introduction were also checked by 4 microsatellite-SSR loci. The variations of the eight agronomic traits were higher in the local landrades than in the inbred line. which was substantiated by the high genetic variation in the landrades with microsatellite-SSR loci. The level of genetic variation was also different between landraces. Since the genetic evaluation can be easily quantified by the analysis of microsatellite-SSR loci. the threshold level of genetic homogeneity in the population for parental lines in breeding program can be determined and the effort of maintaining the landrace population would be alleviated. As an example in our analysis. the entry from Whachon should not need the same number of selfing generations as the other two landraces to get the level of inbred state. Since this line showed lowest intra-genetic variation within the population.

Carotenoid compounds in embryos of wild-type(WT) and viviparous mutants of maize(Zea mays L.) were analyzed using high performance liquid ehromatography (HPLC) with a photodiode array detector. Zeaxanthin accumulates in WT embryos as the major carotenoid. Phytoene accumulates in vp2 and vp5. Phytofluene in w3 and ζ-carotene in the vp9 mutant embryos. This indicates that the vp2 and vp5 mutants impair phytoene desaturase from 15-cis-phytoene to 15-cis-phytofluene. The w3 mutant has neither an isomerase from 15-cis-phytofluene to all-trans-phytofuene nor phytofluene desaturase from phytofluene to ζ-carotene. The vp9 mutant does not have the ζ-carotene desaturase from ζ-carotene to lycopene. Our analysis shows that the terminal carotenoid. γ-carotene(β,ψ-carotene), accumulates in the vp7 mutant embryos. The ε-carotene(ε,ε-carotene), a product of δ-carotene(ε,ψ-carotene) in some plants, however, has not been found in maize embryos. The vp7 mutant impairs a cyclization step from γ-carotene to both β-carotene and α-carotene. We suggest that monocyclic γ-carotene is the sole precursor of both bicyclic β-carotene(β,β-carotene) and α-carotene(β,ε-carotene) in maize.

One of the most attractive short-term possibilities for increasing freezing tolerance of winter crops may be the application of chemicals. This research was conducted to determine the effect of two plant growth regulators. Terpal-C and Cerone on freezing tolerance and winter survival of canola. Three cultivars were planted on the michigan state University Agronimy Farm at East Lansing. MI. on Sept. 10. 1992 and 1993 Chemicals were applied to one-month-old plants when they reached the 5 leaf stage. Ion leakage tests for freezing tolerance were conducted before and after chemical treatment. Winter survival was evaluated by counting the plant standing in the fall and spring. Neither of the chemicals. Terpal-C. inhibited natural cold hardening.

The objective of this study was to determine how elevated CO2 and temperature affected early growth and competition between direct seeded rice (Oryza sativa) and a common paddy weed (Echinochloa glabrascens). By using temperature gradient chambers. Rice and E. glabrescens were grown for 5 weeks at ratios of 1:0. 3:1 and 0:1 at three temperatures (16.4℃, 19.8℃, and 22.2℃) and either in ambient (361ppm) or elevated (566ppm) CO2. For both species. elevated CO2 had no effect on mainstem leaf number while air temperature had a slight positive effect which was greater in E. glabrescens than rice. With elevated CO2 rice leaf area index and plant height increased alightly in all species combinations but no increases were observed for E. Glabuescens. For rice in all combinations. elevated CO2 tended to increase the rot and total biomass much more than any other growth parameters: the increases in root and total biomass resulting from elevated CO2 ranged from 16% to 40%. depending on air temperature. At the lowest temperature, the decrease in rice biomass in combination with E. glabrescens was significantly greater at elevated CO2 (18%) than ambient CO2 (3%). At the highest temperature, however, the decrease in rice biomass at elevated CO2 (22%) was less than that at ambient CO2 (36%). The competitive ability of rice as measured by the decrease in biomass when grown in combination with E. glabrescens depended strongly on root growth and/or allocation. These results suggest that at higher temperatures elevated CO2 could enhance the competitive ability of direct seeded rice during early growth. However, at lower temperatures. the competitive ability of E. glabrescens seems to be greater.

Laboratory experiments for the removal efficiency of heavy metals in land application of sludge, the accumulation and translocation of heavy metals in x plants after transplanting, and the responses of Minari growth with different ratio of land application of sludge were conducted to determine the potential ability of bioremediation with Minari plants. The removal rate and translocation of copper. zinc. lead. and cadmium in soil and plants were compared after transplanting the Minari plants to soil treated with different ratio of sludge. The removal efficiency of heavy metals in soil incorporated with sludge was different with application ratio, but increased with growing periods of Minari plants. The removal efficiency of Cu, Zn, Pb, and Cd ranged from 67 to 74% from 51% to 63%, from 37% to 71%. and from 15% to 25% after 45 days of transplanting. respectively. The amount removed the copper value. 65.9 mg/kg, observed to be highest in soil incorporated 3% sludge after 45 days. The translocation of Cu. Zn. Pb. and Cd from shoots to roots ranged from 18 to 53%, from 17 to 32%, from 14 to 49%, and from 23 to 38% over growing periods. respectively. In plant responses it appeared to be inhibited the plant growth in the treatment compared with the control at early stage of growth. However, the fresh weights of Minari plant increased from 12.5 to 62.5% in the sludge application after 45 days relative to the control. Therefore the Minari might play a useful role in bioremediation of Cu, Zn, Pb, and Cd in the land application of sludge.